15N示踪-离子质谱法测定新生产力的研究

本文提出了~(15)N示踪-离子质谱法实测新生产力的方法,并于1991年11月—1992年5月结合胶州湾现场调查的实际样品,对示踪剂量、培养时间、样品贮存、消解剂量和时间、铵离子的扩散吸收,以及方法的空白值、检出限、精密度和准确度等进行了实验研究。结果表明,该法具有较高的精密度和良好的准确度,与经典的气体质谱法相比,还具有进样量少以及可同时分析~(15)N丰度、总氮浓度和水中铵离子浓度等特点,对于含氮量极低、铵氮再生迅速的海洋环境中新生产力的研究,具有特殊的意义。     

On the long-term joint distribution of characteristic wave height and period and its application

By analysing the scatter diagrams of characteristic the wave height H and the period T on the basis of instrumental data from various ocean wave stations, we found that the conditional expectation and standard deviation of wave period for a given wave height can be better predicted by using the equations of normal linear regression rather than by those based on the log- normal law. The latter was implied in Ochi' s bivariate log-normal model(Ochi. 1978) for the long-term joint distribution of H and T. With the expectation and standard deviation predicted by the normal linear regression equations and applying proper types of distribution, we have obtained the conditional distribution of T for given H. Then combining this conditional P(T / H) with long-term marginal distribution of the wave height P(H) we establish a new parameterized model for the long-term joint distribution P(H,T). As an example of the application of the new model we give a method for estimating wave period associated with an extreme w     

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

潮汐分析和预报的准调和分潮方法 Ⅱ.短期观测的分析

目前,调和方法仍然是潮汐的分析和预报中的一种主要的基本方法,其关键在于求得尽可能准确的调和常数。在湖汐分析中,经常遇到的是对于中、长期(半个月以上)记录的处理,对于这样的资料,已有许多良好的分析方法可以采用。但是,有时我们也会遇到需要处理的短期观测资料,特别对于潮流,由于对它进行观测比水位观测要困难,常常不能获得较长期的记录,而需要从短期记录中分析出调和常数来。处理短期记录的方法也有不少,其中较为常用的是英国海军部的几种方法。这些方法只能处理一次或二次周日观测记录,当观测天数较多时,只能分组进行计算得到几组调和常数,然后再求平均值由于各组的天文条件的差別,调和常数的准确度常常有相当大的差异,而计算平均值时却没有加权,这就影响了结果的可靠性。本文建议的方法将可以直接分析任意多次周日观测记录,因而简化了计算并能得到更准确的结果。     

文昌鱼哈氏窝匀浆使蟾蜍足细胞释精的超微结构研究

本文报道了文昌鱼哈氏窝匀浆刺激蟾蜍足细胞释放精子过程所发生的超微结构变化,进一步证实这种匀浆含有类似脊椎动物促黄体生成素活性物质。     

Studies on ultrastructure of oocyte in process of maturing in pen shell

The present paper deals with the ultrastructural changes of oocyte in the process of maturing in pen shell Atrinapectinata Linne. When it develops from the antecedent growth period to the accelerating growth period the annulate lamellae emerge, the RER better develop, the yolk granules with single and double layer membrane and are visible. In the well-developed mitochondria there is yolk stored. The Golgi vacuoles are brewing yolk granules. At the mature period the annulate lamellae and the Golgi bodies disappear, and there are only a few RER and mitochondria with poor cristae in the cytoplasm. All these might be well used for judging the maturity of the ootids in the process of the artificial propagation.     

Study of zooplankton ecology in Zhejiang coastal upwelling system-Zooplankton biomass and abundance of major groups

The studied area is a shallow water area that belongs to the East China Sea continent shelf. The distribution of zooplankton biomass is higher inshore than offshore. The maximum abundance is in the inshore edge of the centre of upwelling, which is the superposed area of the salinity front and temperature front, due to the fact that the mixture of three different waters has brought about a concentration of nutrients. The herbivorous Euphausia, Copepoda and Tunicata are the major groups of the abundant area. Tunicata possess the possibility to compete against other kinds of herbivorous zooplankton. This means that there is negative correlation between the distribution of Tunicata and that of Copepoda and Euphausia. The positions of maximum areas of zooplankton biomass, phytoplankton individual and the concentration of phosphates and chlorophyll-a overlap one another. In the centre of upwelling, zooplankton can not adapt itself to the environment of lower temperature and less oxygen even with rich nutrient     

海带的一个自然突变型

在多细胞藻的遗传学研究中,被发现的突变型很少[1].绿藻中有一些突变型,其中较多的是某种石莼(Ulva mutabilis)[2].三浦昭雄等在紫菜中发现突变型.在褐藻中未见有突变型的报道.这里所报道的是海带(Laminaria japonica)的一个突变型.     

台湾海峡上升流区底栖生物数量动态的初步研究

不同季节底梄生物数量的动态由其组成种的种群特点所决定。在一些生命周期短(一年左右)、生长速度快(新生个体当年可以长成)的种处于优势的情况下,生物数量的季节变化就比较显著;而生命周期长、其种群由多个年龄组的个体构成的种占优势的情况下，一年之中种群数量就相对稳定。另一方面,底栖生物数量的急剧改变又决定于经济种的捕食活动和优势种的生物学过程。台湾海峡系经济鱼、虾类的集中分布区,尤以上升流区范围内的集群最密集。为了便于深入研究上升流与经济鱼、虾类资源的关系,本文着重探讨上升流范围内底栖生物数量的动态特点。     

春季东海不同水域的表层叶绿素含量

利用１９９４年春季在东海及台湾海峡首次获得的表层水中绿素含量大面走航连续观测资料，结合温盐分布及以往的有关东海及台湾海峡的海流和上升流的结论，分析了春季东海表层叶绿素含量在不同水域的分布特征。结果表明，叶绿素含量分布与水文结构关系密切。叶绿素含量值随不同海流流域而变，但在各海流流域内基本不变，黑潮表层水的叶绿素含量最低，其次是台湾暖流表层水，长江冲淡水与江浙沿岸流域的叶绿素含量较高；黑潮西侧弱流剧