热球菌HJ21高温α-淀粉酶基因克隆、表达及性质研究

通过简并引物扩增热球菌(Thermococcus siculi)HJ21高温α-淀粉酶保守区域之间的序列,再利用Site-finding技术获得两端未知序列.构建了在N端添加了His6标签的表达载体pEt-28a-His6-THJA后转化E.coli,在IPTG诱导下表达.进一步纯化后SDS-PAGE电泳检测达到电泳纯...     

文昌鱼精子发生的核孔复合体冷冻蚀刻电镜观察

1994-1996年每年3月、7月在厦门同安琼头海域采集雄性文昌鱼,采用冷冻蚀刻的常规技术,研究文昌鱼精子发生核孔复合体的变化规律。结果表明,在核孔复合体结构中很可能存在着作为运输物质的中央颗粒。各种生殖细胞中的核孔复合体的大小和密度不同,平均值约为:精原细胞（60±1.20）nm,（2.9±0.04）孔／μm2;初级精母细胞（76±1.80）nm,（4.8±0.05）孔／μm2;次级精母细胞（72±1.30）nm,（4.6±0.03）孔／μm2;精细胞（97±1.40）nm,（5.4±0.06）孔／μm2。这与细胞的生理功能有关。核孔复合体的分布具有明显的极性特点,主要集中在靠初级精母细胞的高尔基区一侧和靠精细胞的前项体一侧的核膜上,表明了细胞核局部的功能差异。     

Terrestrial photogrammetric techniques applied to the control of a parabolic dune in the Liencres dune system,Cantabria (Spain)

The spatial–temporal variations of a dune system can be determined by using diverse ‘geomatic’ methodologies: geodesy, global positioning system (GPS) and photogrammetry. In the case of the Liencres dune system, a study will be carried out using the ‘close‐range’ photogrammetry technique and the topography technique (total station and GPS). In order to determine the dynamic of the dune system it is necessary to repeat the process of study after a specific interval of time. For this reason, three dimensional data should be available in two different time periods, between which the displacement of the object of analysis (the front portion of the dune) will be significant enough to evaluate its magnitude. This work analyses the viability of photogrammetry for the determination of the spatial–temporal changes of a coastal parabolic dune. Two factors have been analysed: first, the comparison of the photogrammetric results with the results obtained from topographic methods (total station and GPS), and second, the quantification of the displacement of the dune system. The analysis of the correspondence between the movement of different parts of the dune and the influence of the intensity and direction of the prevailing wind in the area is also desired. The dune advanced 12·15 ± 0·06 m (an average of 8·5 m/yr), and the partial implications for the dynamic of human modified processes on the natural park have been established. Copyright © 2008 John Wiley & Sons, Ltd.     

新疆柯坪新生代推覆岩席的走向连接与成因分析

柯坪断隆新生代推覆构造是南天山南缘冲断系统的一部分,断隆上多排逆冲岩席被数条横向断裂复杂化,致使不同地段岩席排数不等,各排岩席在走向上的对应关系不明,形成了十分奇特的山弯构造.逆冲岩席的变形特点是南天山新生代造山运动的重要表征.笔者通过卫星影像分析和野外路线地质调查,对横向断裂切割各排逆冲岩席之切点部位的构造特征及岩席内部中小型构造所显示的成因信息进行了详细分析,据此,对复杂岩席在走向上进行了连接,对其数量进行了识别.在此基础上,提出了复杂岩席成因的斜冲褶皱模式,即在北西向应力场持续作用下,岩席向南东斜冲,并在其水平位移分量作用下,沿走向发生褶皱和斜冲错断:横向断裂是多排岩席褶皱翼部扭性断层的发展和连通.     

Hydrostratigraphic framework and hydrogeological behaviour of the Mancha Oriental System (SE Spain)

The Mancha Oriental System (MOS) has a surface area of 7,260 km², making it one of the largest carbonate aquifer systems in Spain. The system sustains about 1,000 km² of irrigated crops and supplies groundwater to 275,000 inhabitants. The economic transformation brought about by the development of extensive irrigated cropland has led to a water-balance disequilibrium of about 75 million m³/year. This input–output deficit has negative consequences in the quantity and quality of the available resources, in the river–aquifer relationship, and in the associated ecosystems as well. To understand the hydrogeological behaviour of the system, it is necessary to design a conceptual model. Further, the conceptualisation of a groundwater flow system is a requirement of the European Water Framework Directive for the characterisation of groundwater bodies. The robustness of the conceptual model depends heavily on the user capability of representing the real system. In this work, a multidisciplinary approach has been used to represent the three-dimensional geological framework and the groundwater flow conceptualisation of the MOS. Data management and three-dimensional visualisation have been carried out by means of geographical information system (GIS) tools and software for contouring and three-dimensional surface mapping.     

GPS卫星轨道精度对定位结果的影响

卫星轨道误差是影响定位精度的重要误差源,在轨道精度、表示方式和实时性等方面对广播星历、超快速星历、快速星历和精密星历进行了比较分析,得出广播星历与精密星历所提供的卫星轨道相差较大,超快速星历居中,快速星历轨道与精密轨道接近,在测量设备的高精度比对鉴定中,满足不同试验需求对卫星星历的选择提供了依据。     

Trophic ecology of Mustelus schmitti (Springer, 1939) in a nursery area of northern Patagonia

Mustelus schmitti is an endangered endemic shark of the southwest Atlantic, and an important economical resource in Argentina, Brazil and Uruguay. The objective of this study was to describe the trophic ecology of M. schmitti in Anegada Bay, its feeding strategy and diet composition, along with the possible dietary shifts, due to season, sex, ontogeny and the different geographical features of the bay. Our results show that M. schmitti is a carnivorous opportunistic predator, feeding on a variety of benthic invertebrates. The diet presented seasonal and ontogenetic variations, while no differences in diet composition were observed between sexes or the different sampling sites. This species behave as a generalize feeder, with a wide trophic spectrum and a diverse diet.     

Chronological Study of Coal-seam Water and its Implication on Gas Production in the South Qinshui Basin

The knowledge of the residence time of formation water is fundamental to understanding the subsurface flow and hydrological setting. To better identify the origin and evolution of coal seam water and its impact on gas storage and production, this study collected coalbed methane co-produced water in the southeast Qinshui Basin and detected chemical and isotopic compositions, especially 36Cl and 129I concentrations. The calculated tracer ages of 129I (5.2–50.6 Ma) and 36Cl (0.13–0.76 Ma) are significantly younger than the age of coal-bearing formation (Pennsylvanian - Cisuralian), indicating freshwater recharge after coal deposition. The model that utilises 129I/I and 36Cl/Cl ratios to constrain the timing of recharge and the proportion of recharge water reveals that over 60% of pre-anthropogenic meteoric water entered coal seams since 10 Ma and mixed with residue initial deposition water, corresponding to the basin inversion in Cenozoic. The spatial distribution of major ion concentrations reveals the primary recharge pathway for meteoric water from coal outcrops at the eastern margin to the basin center. This study demonstrates the occurrence of higher gas production rates from wells that accept water recharge in recent times and suggests the possible potential of the non-stagnant zones for high gas production.     

Effect of the Coefficient on the Performance of A Two-Layer Boussinesq-Type Model

The coefficients embodied in a Boussinesq-type model are very important since they are determined to optimize the linear and nonlinear properties.In most conventional Boussinesq-type models,these coefficients are assigned the specific values.As for the multi-layer Boussinesq-type models with the inclusion of the vertical velocity,however,the effect of the different values of these coefficients on linear and nonlinear performances has never been investigated yet.The present study focuses on a two-layer Boussinesq-type model with the highest spatial derivatives being 2 and theoretically and numerically examines the effect of the coefficient on model performance.Theoretical analysis show that different values for(0.13≤α≤0.25)do not have great effects on the high accuracy of the linear shoaling,linear phase celerity and even third-order nonlinearity for water depth range of 0相似文献   

Methods and procedures to determine the outer limits of the continental shelf beyond 200 nautical miles

This paper establishes techniques and methods to determine a variety of boundaries associated with 200 nautical miles beyond the continental shelf. The methods, based on topography, slope and second-deriv- ative profile integrated analysis, are now able to identify automatically the foot of the continental slope （FOS）. By analyzing the sedimentary profile, the points of 1% sediment thickness are recognized. Through the intersection, cut, deletion and mergence calculation of the extrapolated data set of fLxed-point series, the method succeeds in generating automatically the extrapolated boundaries, including the FOS＋60 M line, the 350 M line, and the 2500 m＋100 M line. In addition, based on the automatic analysis of the topographic profile, it can be applied to determine rapidly the points of maximum water depth. Taking the northern Okinawa Trough （OT） as an example, these methods are used to calculate and examine the boundaries in- cluded in the Submission by the People＇s Republic of China Concerning the Outer Limits of the Continental Shelf beyond 200 Nautical miles in Part of the East China Sea （ECS）; the boundaries thus derived have a solid scientific and rational basis.