Variability of the reverse-flow concentration technique of measuring plankton respiration

Results from the reverse-flow concentration method used to determine community plankton respiration vary significantly because of metabolic inactivation or losses of a portion of the concentrated sample during the filtering process. These losses seem to be nearly constant at concentrations greater than 150X. The within sample coefficient of variation is 20–40% and is primarily a result of clumping and inadequate mixing before subsampling. A rapid processing of the samples is critical for best results.     

Properties of pressure fluctuations in an atmospheric boundary layer

From experimental data it is shown that there is a relationship between measured wind speed and associated micropressure levels. It is also shown that turbulent eddies associated with the wind vanish within distances of five to six times their size, and that these eddies exhibit normal dispersion. These results confirm, at the atmospheric scales, results previously observed only in model experiments.     

Particulate dispersion from sources within a forest

Particulate dispersion from sources within a 10- to 13-m tall pine forest was studied experimentally at Brookhaven National Laboratory using stained ragweed pollen and other tracers ranging from 14 to 58 m in size. Forty-seven continuous point source releases lasting from 22 to 55 min were made at heights from 1.75 to 14.0 m from locations having a long fetch through the forest. In most experiments, differently colored ragweed pollen were emitted simultaneously from three locations. In other tests, several particle types were released from a single point. The sampling network consisted of 119 rotoslide samplers at heights from 0.5 to 21.0 m at 57 positions within and at the edge of the forest. Deposition to the ground was sampled by greased microscope slides at each position. Meteorological measurements were taken in and near the forest.Data were classified by particle characteristics, source height and meteorological parameters. Concentration patterns were illustrated on scale diagrams of the sampling grid. Changes in centerline and crosswind integrated concentrations, plume width and height, mass flux, deposition and deposition velocity were studied as a function of distance, particle size and wind speed. Results were compared to those obtained from similar releases over open terrain.In the forest, vertical predominates over lateral dispersion and considerable interchange occurs through the canopy. Flow is channelled somewhat by vegetation density differences but is generally in the direction of the mean wind above the forest. No systematic turning of the wind with height was observed. Most particles are lost to the foliage rather than to the ground and large particles are lost more rapidly than smaller ones. Rate of change in mass flux is similar to that over open terrain and is greater with light than with stronger wind speeds.This research was carried out under the auspices of the New York State Museum and Science Service and the U.S. Atomic Energy Commission (now Energy Research and Development Administration) and was partially supported by Research Grant No. R-800677 from the Division of Meteorology, U.S. Environmental Protection Agency.     

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Seasonal distribution of rainfall in Australia analysed by Fourier methods

Summary A harmonic or Fourier analysis of mean monthly rainfall data is found useful in objectively describing and mapping the seasonality of rainfall within Australia. Over large areas the seasonal component is well described by the first and second harmonic terms, leaving as a residual variance only a small proportion of the observed total variance. Care must be taken in the interpretation of second harmonic contributions within those areas of tropical Australia under strong monsoonal influences. This is because pecularities in the form of the annual rainfall regime make the first harmonic term inadequate in itself to fully describe the annual trend. The technique is found particularly useful in defining the boundaries of areas of similar seasonality, and maps based upon the first and second harmonic amplitudes are shown to bear a close relationship with known physical controls.

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Zusammenfassung Zur objektiven Beschreibung der jahreszeitlichen Schwankung der Regenfälle in Australien und ihrer kartenmäßigen Darstellung wird mit Erfolg die harmonische Analyse der mittleren Monatsmengen des Niederschlags angewendet. Über weiten Gebieten wird die jahreszeitliche Schwankung durch die ersten beiden harmonischen Schwingungen gut dargestellt; die Reststreuung macht nur einen kleinen Bruchteil der beobachteten Gesamtstreuung aus. Vorsicht erscheint jedoch geboten bei der Interpretation des Anteils der zweiten Harmonischen in den Gebieten des tropischen Australien, die unter starkem Monsuneinfluß stehen. Hier machen Besonderheiten im Niederschlagsregime selbst die erste Harmonische ungenügend zur Beschreibung des Jahresganges. Die angewandte Methode ist besonders geeignet, die Grenzen zwischen Gebieten mit gleichartigem Jahreszeiteneinfluß festzulegen. Karten, die auf der ersten und zweiten harmonischen Schwingung beruhen, zeigen einen engen Zusammenhang mit bekannten physikalischen Faktoren.

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Résumé Afin de décrire objectivement les variations saisonnières des précipitations en Australie, on se sert avantageusement de l'analyse harmonique des sommes mensuelles moyennes. Cette méthode est également utilisée pour la représentation cartographique des dites variations. Les amplitudes saisonnières sont bien rendues par les deux premières harmoniques et cela pour de grandes étendues. L'amplitude qui reste ne représente qu'une faible fraction de l'amplitude totale observée. Il faut cependant procéder avec prudence lors de l'interprétation de la part revenant à la seconde harmonique dans les contrées tropicales de l'Australie, contrées qui subissent fortement l'influence de la mousson. Dans ce cas précis, les particularités du régime des précipitations ne permettent même pas d'utiliser la première harmonique pour décrire l'évolution des pluies au cours de l'année. La méthode proposée est surtout intéressante pour délimiter les régions soumises aux mêmes régimes. Des cartes basées sur la première et la seconde harmonique montrent un rapport étroit entre les précipitations et certains facteurs physiques connus.

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With 9 Figures     

Area and volume computation of longitude–latitude grids and three‐dimensional meshes

Longitude–latitude grids are commonly used for surface analyses and data storage in GIS. For volumetric analyses, three‐dimensional meshes perpendicularly raised above or below the gridded surface are applied. Since grids and meshes are defined with geographic coordinates, they are not equal area or volume due to convergence of the meridians and radii. This article compiles and presents known geodetic considerations and relevant formulae needed for longitude–latitude grid and mesh analyses in GIS. The effect of neglecting these considerations is demonstrated on area and volume calculations of ecological marine units.     

An atlas of ground UV spectra of selected stars

We present a spectral atlas of 4 B and A stars containing spectra in a poorly studied spectral range of 305–452 nm. The atlas is based on high resolution (R=60 000) spectra obtained with the 6 meter telescope (SAO, Russia) combined with the NES-spectrograph. The procedure of spectral lines identification and compilation of the atlas is discussed in detail. Using the spectral data we thoroughly investigated the velocity field in expanding atmospheres and envelopes of hot evolved stars β Ori, α Cyg and supergiant KS Per with the extreme hydrogen deficiency. The complete atlas and list of the identified spectral lines will be available via the astronomical database SIMBAD.     

Electrochemical detection of harmful algae and other microbial contaminants in coastal waters using hand-held biosensors

Standard methods to identify microbial contaminants in the environment are slow, laborious, and can require specialized expertise. This study investigated electrochemical detection of microbial contaminants using commercially available, hand-held instruments. Electrochemical assays were developed for a red tide dinoflagellate (Karenia brevis), fecal-indicating bacteria (Enterococcus spp.), markers indicative of human sources of fecal pollution (human cluster Bacteroides and the esp gene of Enterococcus faecium), bacterial pathogens (Escherichia coli 0157:H7, Salmonella spp., Campylobacter jejuni, Staphylococcus aureus), and a viral pathogen (adenovirus). For K. brevis, two assay formats (Rapid PCR-Detect and Hybrid PCR-Detect) were tested and both provided detection limits of 10 genome equivalents for DNA isolated from K. brevis culture and amplified by PCR. Sensitivity with coastal water samples was sufficient to detect K. brevis that was "present" (相似文献   

High- and low-affinity binding sites for Cd on the bacterial cell walls of Bacillus subtilis and Shewanella oneidensis

Bulk Cd adsorption isotherm experiments, thermodynamic equilibrium modeling, and Cd K edge EXAFS were used to constrain the mechanisms of proton and Cd adsorption to bacterial cells of the commonly occurring Gram-positive and Gram-negative bacteria, Bacillus subtilis and Shewanella oneidensis, respectively. Potentiometric titrations were used to characterize the functional group reactivity of the S. oneidensis cells, and we model the titration data using the same type of non-electrostatic surface complexation approach as was applied to titrations of B. subtilis suspensions by Fein et al. (2005). Similar to the results for B. subtilis, the S. oneidensis cells exhibit buffering behavior from approximately pH 3-9 that requires the presence of four distinct sites, with pK_a values of 3.3 ± 0.2, 4.8 ± 0.2, 6.7 ± 0.4, and 9.4 ± 0.5, and site concentrations of 8.9(±2.6) × 10⁻⁵, 1.3(±0.2) × 10⁻⁴, 5.9(±3.3) × 10⁻⁵, and 1.1(±0.6) × 10⁻⁴ moles/g bacteria (wet mass), respectively. The bulk Cd isotherm adsorption data for both species, conducted at pH 5.9 as a function of Cd concentration at a fixed biomass concentration, were best modeled by reactions with a Cd:site stoichiometry of 1:1. EXAFS data were collected for both bacterial species as a function of Cd concentration at pH 5.9 and 10 g/L bacteria. The EXAFS results show that the same types of binding sites are responsible for Cd sorption to both bacterial species at all Cd loadings tested (1-200 ppm). Carboxyl sites are responsible for the binding at intermediate Cd loadings. Phosphoryl ligands are more important than carboxyl ligands for Cd binding at high Cd loadings. For the lowest Cd loadings studied here, a sulfhydryl site was found to dominate the bound Cd budgets for both species, in addition to the carboxyl and phosphoryl sites that dominate the higher loadings. The EXAFS results suggest that both Gram-positive and Gram-negative bacterial cell walls have a low concentration of very high-affinity sulfhydryl sites which become masked by the more abundant carboxyl and phosphoryl sites at higher metal:bacteria ratios. This study demonstrates that metal loading plays a vital role in determining the important metal-binding reactions that occur on bacterial cell walls, and that high affinity, low-density sites can be revealed by spectroscopy of biomass samples. Such sites may control the fate and transport of metals in realistic geologic settings, where metal concentrations are low.     

Utilization of a multicriteria least cost path model in an aquatic environment

A critical component of maintaining biodiversity in fragmented habitats is maintaining connectivity among the usable fragments. Least cost path (LCP) analysis is a tool that can be used for predicting the ability of an organism to move from one habitat patch to another, based on geographical features of the landscape and life history traits of the organism. While this analysis has been utilized for terrestrial habitats, it is rarely applied to aquatic environments. Aquatic hypoxic conditions occur when dissolved oxygen falls below 2 mg/L. These conditions can create barriers in the water column that can either force fish to leave a habitat, or avoid that habitat altogether. Using the lower St. Johns River (LSJR) estuary in Florida, USA, as a study system, the ability of an adult silver perch, Bairdiella chrysoura, to escape a large-scale hypoxic event was modeled using a multicriteria LCP approach. Criteria-specific cost grids were constructed based upon current speed, risk of predation, and whether oxygen levels in the habitat area were normoxic (>5.5 mg/L), or hypoxic (<2.0–1.5 mg/L) as a function of water depth for the LSJR. The criteria cost grids were combined using relative weighting to produce the multicriteria cost grid used to implement the LCP analysis. Three origin and destination point locations within the LSJR study area were selected for modeling whether or not a silver perch would be able to escape a hypoxic zone. Since the LCP model will always determine a LCP from the specified origin point location, ecologically relevant swimming capacities for silver perch under normoxic and hypoxic conditions were then applied to assess the model, and to determine whether the fish would be able to reach areas unimpacted by hypoxia. The LCP model and the swimming capacity results for this study predict that under normoxic conditions, fish movement was unimpeded. During the rapidly developing hypoxic event that was modeled, the results from the LCP model indicate that the fish could move outside the hypoxic zone, but when swimming capacities were applied to the model, the silver perch could not escape. Ecologically, the results of this study suggest that silver perch would experience high mortality under a rapidly developing hypoxic event. Additionally, the results of this study indicate that a LCP model can be applied to an aquatic habitat, as long as the cost grids incorporate relevant abiotic and biotic factors.