Modelling phytoplankton succession on the Bering Sea shelf: role of climate influences and trophic interactions in generating Emiliania huxleyi blooms 1997–2000

Several years of continuous physical and biological anomalies have been affecting the Bering Sea shelf ecosystem starting from 1997. Such anomalies reached their peak in a striking visual phenomenon: the first appearance in the area of bright waters caused by massive blooms of the coccolithophore Emiliania huxleyi (E. huxleyi). This study is intended to provide an insight into the mechanisms of phytoplankton succession in the south-eastern part of the shelf during such years and addresses the causes of E. huxleyi success by means of a 2-layer ecosystem model, field data and satellite-derived information. A number of potential hypotheses are delineated based on observations conducted in the area and on previous knowledge of E. huxleyi general ecology. Some of these hypotheses are then considered as causative factors and explored with the model. The unusual climatic conditions of 1997 resulted most notably in a particularly shallow mixed layer depth and high sea surface temperature (about 4 °C above climatological mean). Despite the fact that the model could not reproduce for E. huxleyi a clear non-bloom to bloom transition (pre- vs. post-1997), several tests suggest that this species was favoured by the shallow mixed layer depth in conjunction with a lack of photoinhibition. A top-down control by microzooplankton selectively grazing phytoplankton other than E. huxleyi appears to be responsible for the long persistence of the blooms. Interestingly, observations reveal that the high N:P ratio hypothesis, regarded as crucial in the formation of blooms of this species in previous studies, does not hold on the Bering Sea shelf.     

Application of wavelet transforms to the decoupling of free-roll decay data

This study presents the results of the decoupling of a free-roll decay test by wavelet transforms. A free-roll decay test was performed to determine the coefficients of damping terms in equations of motion. No motion of the model was restricted during the experiment. A slight yaw motion was found while the model was in the free-roll decay motion. It was necessary to extract a pure roll motion from the experimental data. Wavelet transforms were applied to the signals to extract the pure roll motion. The results were compared to those found through the Fourier transform. Discrete wavelet transforms were able to efficiently decouple the test signals, while the continuous wavelet transform and the Fourier transform could not.     

Elucidating the dynamics and mixing agents of a shallow fjord through age tracer modelling

The mixing agents and their role in the dynamics of a shallow fjord are elucidated through an Eulerian implementation of artificial tracers in a three-dimensional hydrodynamic model. The time scales of vertical mixing in this shallow estuary are short, and the artificial tracers are utilized in order to reveal information not detectable in the temperature or salinity fields. The fjord's response to external forcing is investigated through a series of model experiments in which we quantify vertical mixing, transport time scales of fresh water runoff and estuarine circulation in relation to external forcing.Using age tracers released at surface and bottom, we quantify the time scales of downward mixing of surface water and upward mixing of bottom water. Wind is shown to be the major agent for vertical mixing at nearly all depth levels in the fjord, whereas the tide or external sea level forcing is a minor agent and only occasionally more important just close to the bottom. The time scale of vertical mixing of surface water to the bottom or ventilation time scale of bottom water is estimated to be in the range 0.7 h to 9.0 days, with an average age of 2.7 days for the year 2004.The fjord receives fresh water from two streams entering the innermost part of the fjord, and the distribution and age of this water are studied using both ageing and conservative tracers. The salinity variations outside this fjord are large, and in contrast to the salinity, the artificial tracers provide a straight forward analysis of river water content. The ageing tracer is used to estimate transport time scales of river water (i.e. the time elapsed since the water left the river mouth). In May 2004, the typical age of river water leaving the fjord mouth is 5 days. As the major vertical mixing agent is wind, it controls the estuarine circulation and export of river water. When the wind stress is set to zero, the vertical mixing is reduced and the vertical salinity stratification is increased, and the river water can be effectively exported out of the fjord.We also analyse the river tracer fields and salinity field in relation to along estuary winds in order to detect signs of wind-induced straining of the along estuary density gradient. We find that events of down estuary winds are primarily associated with a reduced along estuary salinity gradient due to increased surface salinity in the innermost part of the fjord, and with an overall decrease in vertical stratification and river water content at the surface. Thus, our results show no apparent signs of wind-induced straining in this shallow fjord but instead they indicate increased levels of vertical mixing or upwelling during down estuary wind events.     

Effect of native and invasive cordgrass on Macoma petalum density, growth, and isotopic signatures

Ecosystem engineers can influence community and ecosystem dynamics by controlling resources, modifying the flow of energy or biomass, or changing physical characteristics of the habitat. Invasive hybrid cordgrass (Spartina alterniflora × Spartina foliosa) is an ecosystem engineer in salt marshes in San Francisco Bay, California, U.S.A. that raises intertidal elevations and may be either increasing C₄ plant carbon input into food webs or tying up carbon in a form that is not usable by consumers. A manipulative experiment compared abundance, growth, and stable isotope (δ¹³C and δ¹⁵N) composition of the clam Macoma petalum (=M. balthica) among native marsh, hybrid Spartina, and mudflats in central San Francisco Bay. We found higher densities (individuals m⁻²) of M. petalum on mudflats compared to either native or hybrid Spartina (p < 0.001). Macoma petalum shell growth was significantly greater in mudflats than in either vegetation type in 2002 (p = 0.005) but not 2003. Differences in shell growth between native and hybrid Spartina were not significant. Stable isotope results showed differences between habitats in δ¹³C but not δ¹⁵N. Carbon signatures of M. petalum placed in Spartina were much more depleted than the isotopic signature of Spartina. Neither native nor hybrid Spartina appears to be a significant carbon source for M. petalum in San Francisco Bay, and we found no evidence that hybrid Spartina contributes carbon to M. petalum beyond what is provided by S. foliosa, despite the hybrid's much greater biomass. Our results show that loss of mudflat habitat, rather than increased input of C₄ carbon, is the greatest effect of the invasion of hybrid Spartina on M. petalum.     

Preliminary attempt to characterize the rotation of seafloor in the Pito Deep area of the Easter Microplate using a submersible magnetometer

Several mechanisms have been proposed to account for the rotation of the nearly north-south abyssal hill fabric formed on the East Pacific Rise north of the Easter Microplate to the nearly east-west trends in the northern microplate interior. Proposed mechanisms include rigid microplate rotation, transform fault – parallel shear, and bookshelf faulting during the transfer of lithosphere from the Nazca Plate to the microplate. We used a submersible magnetometer on a NAUTILE dive program to measure the magnetic vector rotation of a pillow basalt and dike spur near Pito Deep, the present location of the tip of the propagating rift system that created the microplate. Our results, although too limited to draw strong conclusions from, suggest clockwise rotations of the seafloor magnetic vectors inconsistent with the transform-parallel shear model, and larger than can be explained solely by rigid microplate rotation.     

Spatial and temporal variability of microplankton and detritus, and their export to the shelf sediments in the upwelling area off Concepción, Chile (∼36°S), during 2002-2005

Seasonal variations in diversity and biomass of diatoms, tintinnids, and dinoflagellates and the contribution of microplankton and faecal material to the vertical flux of particulates were investigated at one time series station T (station 18) between 2002 and 2005 and at a grid of stations during November 2004 in the coastal and oceanic area off Concepción (36°S), Chile. The variations were analysed in relation to water column temperature, dissolved oxygen, nutrient concentration, offshore Ekman transport, and chlorophyll-a concentration. Abundance was estimated as cell numbers per litre and biomass in terms of biovolume and carbon units.A sharp decrease with depth was observed in the abundance of both phytoplankton and microzooplankton during the whole annual cycle; over 70% of their abundance was concentrated in the upper 10 m of the water column. Also, a clear seasonality in microplankton distribution was observed at station T, with maxima for diatoms, tintinnids, and dinoflagellates every summer (centred on January) from 2002 to 2005.On the grid of stations, the maximum integrated (0-50 m) micro-phytoplankton abundances (>1 × 10⁹ cells m⁻²) occurred at the coastal stations, an area directly influenced by upwelling. A similar spatial distribution was observed for the integrated (0-200 m) faecal carbon (with values up to 632 mg C m⁻²). Tintinnids were distributed in all the first 300 miles from the coast and dinoflagellates were more abundant in oceanic waters.At station T, the average POC export production (below 50 m depth) was 16.6% (SD = 17%; range 2-67%; n = 16). The biological-mediated fluxes of carbon between the upper productive layer and the sediments of the continental shelf off Concepción depend upon key groups of phytoplankton (Thalassiosira spp., Chaetoceros spp.) and zooplankton (euphausiids) through the export of either cells or faecal material, respectively.     

用纤维素乙酸平板电泳测定中国卤虫的同功酶变异(英文)

本文报导了应用纤维素乙酸平板电泳方法,测定采于中国山东省东方红盐场卤虫(Artemia)的同功酶。经测定,8种同功酶其中有6种是多态的,即:苹果酸脱氢酶(MDH)、异柠檬酸酶(IDH)、谷氨酸草醋酸转移酶(GOT)、葡糖-6-磷酸盐脱氢酶(D6PHD)、6-磷酸萄糖脱氢酶(6PGDH)和磷酸葡糖异构酶(PGI)。本文所提供的研究方法,不仅适用于基础研究,而且在水产养殖业上有助于对卤虫的筛选和确证。     

Complexation of mercury by dissolved organic matter in surface waters of Galveston Bay, Texas

The chemical speciation of dissolved mercury in surface waters of Galveston Bay was determined using the concentrations of mercury-complexing ligands and conditional stability constants of mercury-ligand complexes. Two classes of natural ligands associated with dissolved organic matter were determined by a competitive ligand exchange-solvent solvent extraction (CLE-SSE) method: a strong class (L_s), ranging from 19 to 93 pM with an average conditional stability constant (K_HgLs) of 10²⁸, and a weak class (L_w) ranging from 1.4 to 9.8 nM with an average K_HgLs of 10²³. The range of conditional stability constants between mercury and natural ligands suggested that sulfides and thiolates are important binding sites for dissolved mercury in estuarine waters. A positive correlation between the estuarine distribution of dissolved glutathione and that of mercury-complexing ligands supported this suggestion. Thermodynamic equilibrium modeling using stability constants for HgL, HgCl_x, Hg(OH)_x, and HgCl(OH) and concentrations of each ligand demonstrated that almost all of the dissolved mercury (> 99%) in Galveston Bay was complexed by natural ligands associated with dissolved organic matter. The importance of low concentrations of high-affinity ligands that may originate in the biological system (i.e., glutathione and phytochelatin) suggests that the greater portion of bulk dissolved organic matter may not be important for mercury complexation in estuarine surface waters.