Landslides - Landslide-induced tsunamis are common natural hazards that potentially affect the safety and stability of waterfront structures. A numerical tank was constructed using FLOW-3D to... 相似文献
Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
Urban agglomeration is caused by the continuous acceleration of the urbanization process in China. Studying the expansion of construction land can not only know the changes and development of urban agglomeration in time, but also obtain the great significance of the future management. In this study, taking Changsha-Zhuzhou-Xiangtan (Chang-Zhu-Tan) urban agglomeration in Hunan province as a study area, Landsat images from 1995 to 2014 and Autologistic-CLUE-S model simulation data were used. Moreover, several factors including gravity center, direction, distance and landscape index were considered in the analysis of the expansion. The results revealed that the construction area increased by 132.18%, from 372.28 km2 in 1995 to 864.37 km2 in 2014. And it might even reach 1327.23 km2 in 2023. Before 2014, three cities had their own respective and discrete development directions. However, because of the integration policy implementation in 2008, the Chang-Zhu-Tan began to gather, the gravity center moved southward after 2014, and the distance between cities decreased, which was in line with the development plan of urban expansion. The research methods and results were relatively reliable, and these results could provide some reference for the future land use planning and spatial allocation in the urbanization process of Chang-Zhu-Tan urban agglomeration.