南海4种珊瑚附生可培养真菌多样性分析

本研究利用从南海采集的4种珊瑚分离附生真菌,并通过菌株的分类鉴定及系统发育分析获得珊瑚附生可培养真菌的多样性信息.选用酵母麦芽糖琼脂培养基(YM)、马铃薯葡萄糖琼脂培养基(PDA)、马丁氏培养基(Martin)、察氏琼脂培养基(CDA)和孟加拉红培养基(RBM)等5种培养基,采用平板涂布法培养分离附生真菌,基于ITS-rRNA基因序列对真菌菌株进行鉴定和系统发育分析.从4种珊瑚上共分离获得10个属的120株真菌,分别是:青霉属(Penicillum)56株、曲霉属(Aspergillus)25株、枝顶孢属(Acremonium)2株、枝孢属(Cladosporium)5株、毕赤酵母属(Meyerozyma)14株、隔孢伏革属(Peniophora)2株、拟茎点霉属(Phomopsis)1株、裂褶菌属(Schizophyllum)1株、梗孢酵母属(Sterigmatomyces)13株、篮状菌属(Talaromyces)1株.通过对不同珊瑚品种分离获得的附生真菌的比较,发现小叶鹿角珊瑚(Acropora microphthalma)和花鹿角珊瑚(Acropora floride)上分离到的菌株多样性最高,包含6属10种.进一步的分析发现不同珊瑚样品上真菌种类分布有较大差异,隔孢伏革属和裂褶菌属真菌均分离自小叶鹿角珊瑚,梗孢酵母属和篮状菌属均分离自角孔珊瑚(Goniopora sp.),扁脑珊瑚(Platygyra sp.)上则分离到拟茎点霉属.同时还发现不同培养基分离真菌的能力不同,YM培养基分离的菌株最多,CDA培养基分离真菌的多样性最多.以上的研究结果表明,南海珊瑚上存在大量且丰富的真菌资源,可以为后续生物活性物质的开发利用提供良好的菌种资源.     

基于LANDSAT 8卫星热红外影像反演珠江口海表温度

海水表面温度是研究气候变化的重要参数,具有巨大的研究意义.在近海海域进行研究时对分辨率具有较高的要求.在珠江口区域,利用LANDSAT 8影像热红外波段,结合美国中等分辨率成像光谱仪(MODIS)的近红外水汽二级产品MOD05,运用Jiménez-Mu1oz提出的单通道法,优化了部分参数,建立海表温度的反演流程.为了确定可行性,将与LANDSAT 8影像同一日期的MODIS海表温度产品MOD28作为实验的对照组,利用实测数据对比验证2组数据的质量.结果表明,反演结果时空分布规律与MOD28趋近一致,均呈现为夏季西高东低而冬季西低东高,全年温度变化接近准正弦曲线,并且反演结果精度更优,具有良好的应用前景.     

一种高Q值单频水声信号放大提取电路的设计

针对水声信号所处环境复杂、背景噪声源多的特点,提出了一种基于JFET的超低噪音放大电路,该电路选用两片低噪音的场效应管2SK170作为第一级放大器件,经过CR高通滤波连接到同相放大电路,整体电路增益可达70dB;为进一步提升接收信号信噪比,结合双T滤波器特点优化设计出一种高Q值选频电路;通过Multisim14仿真表明,设计的电路在1 Hz处的电压噪声密度仅为2.4 nV/Hz^0.5,滤波器Q值可达25.6,结合实际电路测试结果表明,设计的电路能够满足水声测量要求。     

Retrieval of atmospheric mass densities in lower thermosphere below 200 km from precise orbit of re-entry object CZ-3B R/B by analytical and numerical methods

Taking the re-entry object CZ-3B R/B (COSPAR identifier 2012-018D, NORAD catalog number 38253) as an example, retrieval of atmospheric mass densities in lower thermosphere below 200 km from its rebuilt precise orbit is studied in this paper. Two methodologies, i.e. analytical and numerical methods, are adopted in the retrieval. Basic principles of these two methodologies are briefly introduced. Based on the short-arc sparse observational data accumulated in the high accuracy re-entry prediction, orbit determinations of re-entry object CZ-3B R/B are performed sectionally, and then its precise orbit is rebuilt. According to the orbit theory, the variation of orbital semi-major axis of re-entry object CZ-3B R/B induced by atmospheric drag perturbation only is derived from the rebuilt precise orbit. In the derivation of secular change of the orbital semi-major axis of re-entry object CZ-3B R/B induced by atmospheric drag perturbation only, the time-span is set as one minute tentatively. And then retrieval results of atmospheric mass densities in lower thermosphere below 200 km by analytical and numerical methods are presented, as well as their bias deviations from the calculated results of the NRLMSISE-00 empirical model of the atmosphere. Setting bias deviation bands, the corresponding ‘confidence coefficients’ of the retrieved atmospheric mass densities with respect to the model values are given. Average bias deviations of the retrieved atmospheric mass densities by analytical and numerical methods from the model values are also calculated respectively. On the whole, the retrieved atmospheric mass densities by numerical method approach to the model values more closely; the differences between the retrieved results and the model values are relatively smaller at the peaks of atmospheric mass densities than the other places.     

同种罗非鱼(Tilapia)不同地区选育群体的遗传多样性分析

采用微卫星标记分析技术,选用36对微卫星引物对罗非鱼原始群体和引种群体进行遗传多样性分析,原始群体为中国水产科学研究院淡水渔业研究中心保种的奥利亚罗非鱼("夏奥1号",ZA)、埃及尼罗罗非鱼("99品系",ZN),引种群体为广西水产研究所从淡水渔业研究中心引进培育的奥利亚罗非鱼群体(GA)和埃及尼罗罗非鱼群体(GN)。结果显示19对引物具备多态性,比率为52.8%,ZA与GA两个群体的平均观测杂合度、平均期望杂合度、平均多态信息含量分别为0.10、0.15、0.12,遗传相似性系数为0.9867,平均近交系数(FST)与基因流(Nm)分别为0.0379、6.3408;而ZN与GN两个群体平均观测杂合度、平均期望杂合度、平均多态信息含量分别为0.37、0.49、0.43,遗传相似性系数为0.7696,平均近交系数(FST)与基因流(Nm)分别为0.1146、1.9309。分析结果表明:(1)两个奥利亚群体中ZA与GA遗传多样性小,群体间遗传距离小,相似度较高,选育出的奥利亚群体遗传结构相对稳定;(2)两个尼罗群体中ZN与GN遗传多样性适中,群体间存在一定的遗传距离,相似度低,ZN与GN两个埃及尼罗群体具备进一步选育出新品系的潜力;(3)基因座UNH896、UNH995、UNH999可应用为鉴别奥利亚群体与埃及尼罗群体的特异性标记。     

非均一相互作用能对超薄膜生长影响的Monte Carlo模拟研究

利用Monte Carlo (MC)模拟技术研究了非均一的吸附原子与基底相互作用能在一定的生长条件下对超薄膜生长过程的影响.非均一相互作用能是由基底表面原子在垂直和水平方向上实际位置与理想晶格原子位置的偏差所造成.本文用高斯分布来表示这种非均一相互作用能.模拟结果表明:非均一相互作用能对超薄膜的生长过程及薄膜的形貌有显著的影响.这种影响同时受到生长条件的限制,在中等温度时相互作用能的非均一性对岛的个数、平均大小的影响最显著;温度的增加在一定程度上可抵御相互作用能的非均一性对薄膜生长的影响.     

增强型AlGaN/GaN高电子迁移率晶体管高温退火研究

深入研究了两种增强型AlGaN/GaN高电子迁移率晶体管(HEMT)高温退火前后的直流特性变化.槽栅增强型AlGaN/GaN HEMT在500 ℃ N₂中退火5 min后,阈值电压由0.12 V正向移动到0.57 V,器件Schottky反向栅漏电流减小一个数量级.F注入增强型AlGaN/GaN HEMT在 400 ℃ N₂中退火2 min后,器件阈值电压由0.23 V负向移动到-0.69 V,栅泄漏电流明显增大.槽栅增强型器件退火过程中Schottky有效势垒     

极端事件再现时间长程相关性与群发性研究

利用百分位阈值方法定义极端事件,从极端事件再现时间的角度,研究了极端事件发生时间间隔的长程相关性.发现若原时间序列具有长程相关性,则它的极端事件再现时间序列也具有长程相关性;计算表明两者的标度指数α相当接近,这一特性与随机产生的再现时间序列有着本质的差别,再现时间序列的长程相关性是由原序列的长程相关性决定的.具有长程相关性的时间序列再现时间的概率分布明显不同于随机序列,其小值再现时间的概率较大,反映出极端事件的群发现象.本文根据这一特征定义了再现时间的群发性指数,发现时间序列的长程相关性是导     

Sub-seasonal variability of Luzon Strait Transport in a high resolution global model

The Luzon Strait is the main impact pathway of the Kuroshio on the circulation in South China Sea (SCS). Based on the analysis of the 1997–2007 altimeter data and 2005–2006 output data from a high resolution global HYCOM model, the total Luzon Strait Transport (LST) has remarkable subseasonal oscillations with a typical period of 90 to 120 days, and an average value of 1.9 Sv into SCS. Further spectrum analysis shows that the temporal variability of the LST at different depth is remarkable different. In the upper layer (0–300 m), westward inflow has significant seasonal and subseasonal variability. In the bottom layer (below 1 200 m), eastward outflow exhibits remarkable seasonal variability, while subseasonal variability is also clear. In the intermediate layer, the westward inflow is slightly bigger than the eastward outflow, and both of them have obvious seasonal and subseasonal variability. Because the seasonal variation of westward inflow and eastward outflow is opposite, the total transport of intermediate layer exhibits significant 50–150 days variation, without obvious seasonal signals. The westward Rossby waves with a period of 90 to 120 days in the Western Pacific have very clear correlationship with the Luzon Strait Transport, this indicates that the interaction between these westward Rossby waves and Kuroshio might be the possible mechanism of the subseasonal variation of the LST.     

Energy and first law of thermodynamics for Born—Infeld—anti-de-Sitter black hole

We calculate the local energy and the energy density of the Reisner--Norstrõm--anti-de-Sitter black hole, study the first law of thermodynamics and show the Smarr formula for the Born--Infeld--anti-de-Sitter black hole. Applying the first law of thermodynamics to the black hole region, we analyse the three energy exchange processes between the black hole region and the outer and the inner regions.