天线相位中心偏差变化及改正模型对精密单点定位精度的影响

利用精密单点定位程序对IGS站的实测数据进行计算,结果表明:平面方向,天线相位中心偏差和变化对精密单点定位精度影响较小;高程方向,天线相位中心偏差可造成厘米级的影响,天线相位中心变化的影响约5 mm;相比相对天线相位中心改正模型,使用绝对相位改正模型具有更多优点,尤其用于高精度GPS授时其精度明显提高.     

Iron Regulation of Wetland Vegetation Performance Through Synchronous Effects on Phosphorus Acquisition Efficiency

Iron-rich groundwater flowing into wetlands is a worldwide environmental pollution phenomenon that is closely associated with the stability of wetland ecosystems. Combined with high phosphorus(P) loading from agricultural runoff, the prediction of the evolution of wetland vegetation affected by compound contamination is particularly urgent. We tested the effects of anaerobic iron-rich groundwater discharge in a freshwater marsh by simulating the effect of three levels of eutrophic water on native plants(Glyceria spiculosa(Fr. Schmidt.) Rosh.). The management of wetland vegetation with 1–20 mg/L Fe input is an efficient method to promote the growth of plants, which showed an optimum response under a 0.10 mg/L P surface water environment. Iron-rich groundwater strongly affects the changes in ecological niches of some wetland plant species and the dominant species. In addition, when the P concentration in a natural body of water is too high, the governance effect of eutrophication might not be as expected. Under iron-rich groundwater conditions, the δ~(13)C values of organs were more depleted, which can partially explain the differences in δ~(13)C in the soil profile. Conversely, the carbon isotope composition of soil organic carbon is indicative of past changes in vegetation. The results of our experiments confirm that iron-rich groundwater discharge has the potential to affect vegetation composition through toxicity modification in eutrophic environments.     

栅格数据地学可视化分析环境的构建分析

以遥感数据、数字高程数据等为代表的栅格数据获取技术的进步,以及栅格数据本身适合地学模拟的特点,使得栅格数据应用越来越广泛.当前以定量计算为主的方法难以有效支撑栅格数据分析任务,将可视化引入,充分利用人机协同优势,形成栅格数据地学可视化分析环境是一个较好的解决途径.但是,栅格数据大数据量的特征会引起属性空间可视化时的遮挡...     

Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD₅₀ of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.     

Adhesion mechanisms of Vibrio fluvialis to skin mucus of Epinephelus awoara

Vibrio fluvialis incubated in trypticase soy broth (TSB) showed stronger adhesion to the skin mucus of Epinephelus awoara than V. fluvialis grown on trypticase soy agar (TSA), and this bacterial adhesion was assessed in terms of saturation kinetics. Treating bacteria with antibody against O-antigens resulted in significantly reduced bacterial adhesion. In the early growth stage, the adhering bacteria numbers increased with incubation time, peaked at 24 h, and then dropped sharply. Prior heat treatment of the mucus at various temperatures resulted in different effects on bacterial adhesion. No remarkable effect on bacterial adhesion was detected after treating mucus with protease K and trypsin, whereas periodic acid treatment significantly enhanced bacterial adhesion. Extracts of V. fluvialis surface structures at high concentrations significantly inhibited bacterial adhesion, while relatively low concentrations enhanced adhesion. V. fluvialis had strong affinity for a high molecular weight mucus component. These results indicate that V. fluvialis adhered strongly to E. awoara mucus by specific adhesion and that this adhesion was influenced by many factors, including bacterial conditions, mucus, and the environment.     

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.     

Statistical analysis of intensity variations in tropical cyclones in the East China Sea passing over the Kuroshio

Journal of Oceanology and Limnology - Intensity variations of the SE-NW-oriented tropical cyclones (TC) in the East China Sea (ECS) passing over the Kuroshio are studied using multi-year...     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Phase characteristic analysis of continuous depth air-gun source wavelet

Air guns are important sources for marine seismic exploration. Far-field wavelet of air gun arrays, as a necessary parameter for pre-stack processing and source models, plays an important role during marine seismic data processing and interpretation. When an air gun fires, it generates a series of air bubbles. Similar to onshore seismic exploration, the water forms a plastic fluid near the bubble; the farther the air gun is located from the measurement, the more steady and more accurately represented the wavelet will be. In practice, hydrophones should be placed more than 100 m from the air gun; however, traditional seismic cables cannot meet this requirement. On the other hand, vertical cables provide a viable solution to this problem. This study uses a vertical cable to receive wavelets from 38 air guns and data are collected offshore Southeast Qiong, where the water depth is over 1000 m. In this study, the wavelets measured using this technique coincide very well with the simulated wavelets and can therefore represent the real shape of the wavelets. This experiment fills a technology gap in China.     

THE URBAN FUNCTION OF SUZHOU ON BASIS OF THE SHANGHAI-SUZHOU RELATIONSHIP

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