瘤背石磺体腔细胞分类、免疫相关酶特性及初步转录组学分析

瘤背石磺(Onchidium struma)广泛分布于潮间带和潮下带,是海洋生物向陆地生物辐射进化的重要代表,也是环境监测的指示生物.为丰富瘤背石磺的基础免疫学资料,本文研究了瘤背石磺体腔细胞的类别、细胞密度和体腔液免疫相关酶活性,并通过RNA-Seq测序获得体腔细胞转录本,进行生物信息学分析.结果表明,瘤背石磺主要有...     

黄河口及邻近海域鱼卵、仔稚鱼群落结构及多样性的年间变化

为了解黄河口及邻近海域鱼卵、仔稚鱼的群落结构动态,分别于2014年、2015年、2017年和2018年的6月利用大型浮游生物网对黄河口及邻近海域进行4个航次的表层水平拖网调查。根据鱼卵、仔稚鱼的种类组成、数量分布等群落结构特征,运用生物多样性指数、聚类分析和皮尔逊相关性矩阵分析等方法对该海域鱼卵、仔稚鱼的资源分布进行了综合研究。结果表明,4年调查共采获鱼卵3 829粒,仔稚鱼234尾,隶属于7目14科15属。鱼卵的主要优势种为鳀（Engraulis japonicus）和短吻红舌鳎（Cynoglossus joyeri）,仔稚鱼的主要优势种为斑鰶（Konosirus punctatus）和虾虎鱼科未定种Gobiidae sp.。生物多样性各指数均以2015年最高,2018年最低。利用R语言对鱼卵、仔稚鱼年间种类组成相似性进行聚类,将4个调查年份划分为3组,各分组之间主要的分歧种均为鳀。生物多样性各指数与环境因子之间皮尔逊相关性分析表明,盐度与鱼卵、仔稚鱼生物多样性的关系较为密切。分析认为,黄河口及邻近海域鱼卵、仔稚鱼群落分布和多样性的年间变化特征体现了鱼类对环境变异的响应。     

A new method for platform design based on parametric technology

A new method for platform design, named parametric platform design method (PPDM), is presented in this paper in order to improve the mobile platform design efficiency. In this method, an integrated parametric system that fully considers the characteristics of the platform is developed, which significantly optimizes the design process with the top–bottom design concept. In the parametric system, the main geometric dimensions are taken as parameters, while the topology between the structure members, the function requirements and the safety requirements are converted into geometric constraints. By geometric constraint solving (GCS), a set of parameters that satisfy all the given constraints are determined, and then the design scheme is obtained by several core algorithms, such as parametric tank subdivision, 3D stability calculation, parametric structure design, FEM preprocessing, etc. The parametric method greatly increases the changeability and the reusability of the platform model, and concurrent design is well supported. As the model is driven by parameters, PPDM is an excellent method for optimization design. As a result, PPDM has incomparable advantages on the design efficiency over traditional methods.     

A method to Estimate Dynamic Responses of VLFS Based on Multi-Floating-Module Model Connected by Elastic Hinges

China Ocean Engineering - Based on the elastic foundation beam theory and the multi-floating-module hydrodynamic theory, a novel method is proposed to estimate the dynamic responses of VLFS (Very...     

平湖油气田低渗透储层测井评价研究

平湖油气田P11储层开发是平湖油气田增储提产的重要目标,也是当前平湖油气田开发面临的难题之一。由于埋藏深,储层物性条件差,试油自然产能低下,储层流体性质复杂,气油比低,凝析油含量高,密度大,储层温度高,压力系数高,给测井解释和评价带来很大困难,对开发方案的实施提出了挑战。根据岩心分析资料,从成岩作用、沉积作用等方面对平湖组放鹤亭P11储层低渗原因进行了分析;对P11储层的测井响应特征进行了总结,对P11储层＂四性＂关系、孔隙度结构进行剖析,对储层孔隙度、渗透率、饱和度等参数进行了研究,得到一套适合P11储层测井解释评价的方法,为P11储层的开发提供参数依据和测井解释服务。     

Diurnal variation of nitric oxide emission flux from a mangrove wetland in Zhangjiang River Estuary,China

The diurnal variation of nitric oxide (NO) emission fluxes from a Kandelia obovata and Avicennia marina mangrove wetland were studied in the Zhangjiang River Estuary Mangrove National Nature Reserve using a dynamic chamber-based technique and a chemiluminescent analyzer. Results from field experiments show that NO emission from K. obovata and A. marina sampling sites reached maximal values of 1.07 ng N m⁻² s⁻¹ and 1.23 ng N m⁻² s⁻¹, respectively after the night tide. Meanwhile NO emission maintained at a steady lower level in daytime for both wetland sites. In laboratory experiments, NO emission from the mangrove wetland soil samples treated with simulated tides in the darkness exhibited higher values than those in the light, therefore it seems that tides and darkness could increase NO emission from mangrove wetlands, while intensive light, high temperature, and dryness in the daytime decreased NO emission. Compared with K. obovata soil samples, the diurnal average NO emission rate of the A. marina site was significantly higher, which was closely related to relatively higher diurnal average CO₂ emission rate, soil available nitrogen content and soil net nitrification rate of the A. marina site. Moreover, soil samples of the A. marina site were more responsive to simulated tides and the addition of nitrogen than those of the K. obovata site.     

锦州湾表层海水微塑料分布特征

海洋微塑料是全球关注的新兴环境问题,海湾由于特殊的地理环境特征,成为微塑料分布研究的热点区域。本研究以锦州湾为研究海域,于2017年10月布设了11个点位开展表层海水微塑料样品采集,在实验室采用湿式氧化法开展样品前处理,应用傅立叶变换显微红外光谱仪分析鉴定微塑料成分。研究结果表明,锦州湾表层水体微塑料平均丰度为(0.93±0.59)个/m³,微塑料数量占全部塑料样品的96.2%。微塑料的主要成分为聚丙烯和聚乙烯,分别占55.0%和23.5%;线状和片状塑料的比例最高,分别占41.7%和26.2%;白色、蓝色和半透明微塑料分别占35.1%、26.0%和21.4%。受水动力条件和陆域河流输入等影响,锦州湾表层水体中微塑料的空间分布整体呈现北部偏高,向南部递减的趋势。     

TOPEX Constrained Solution of Mean Sea Surface by Joint Processing of TOPEX,ERS-1 and GEOSAT Altimetric Measurements

We present an improved crossover adjustment procedure to determine mean sea surface height using TOPEX, 35-day repeat phase ERS-1, Geosat, and 168-day repeat phase ERS-1 satellite altimeter data. The mean sea surface frame defined by the TOPEX data is imposed as certain constraints in our crossover adjustment procedure rather than held fixed as in some other procedures. The new procedure is discussed in detail. Equations are developed to incorporate the a priori information of Topex data as well as other satellite altimeter data. The numerical computation result shows that the rms crossover discrepancies are reduced by an order of 1 cm when the Topex data is not fixed. Furthermore, the computed mean sea surface is less noisy and more realistic than that computed by the traditional procedure.     

牙鲆幼鱼越冬饲养期环境因子研究

本文重点研究牙鲆鱼越冬饲养期间所需的适宜温度和盐度条件,以及牙鲆对酸碱度的耐受力。研究结果表明温度是影响牙鲆在越冬期标准代谢的主要环境因子。７～１４．５℃是越冬期牙鲆能量代谢变动最低的温度区间,也是最适宜的温度范围。盐度也是影响牙鲆越冬期的环境因子,在７～１４．５℃的温度条件下,其盐度阈值下限为２０;４０仍属适宜盐度。牙鲆的耐酸力比耐碱性强,９６ｈ和４８ｈ致死的ｐＨ值分别为４．４５和４．２０,当ｐＨ值为９．３０时,牙鲆１６ｈ死亡。