基于MALDI-TOF MS快速鉴定弧菌的数据库的扩展和应用

近年来由海洋细菌导致的感染越来越常见,弧菌是海洋细菌中最常见的细菌之一。目前,对弧菌缺乏快速有效的鉴定。基质辅助激光解吸电离飞行时间质谱仪（Matrix-assisted laser desorption ionization–time of flight mass spectrometry,MALDI-TOF MS）已成为快速鉴别细菌强有力的工具。然而,它的准确性取决于数据库中的菌株数量。本研究中,我们利用MALDI-TOF MS对弧菌数据库进行了扩展和应用。我们将74株弧菌纳入了新的数据库,构建了基于RpoB序列的进化树（邻接法和最大似然比法）,并针对扩展后数据库和扩展前数据库进行了聚类分析。我们利用30株弧菌,对扩展后数据库进行了评价,并进行了主成分分析。本研究中,我们创建了一个新的数据库,促进了弧菌的快速准确鉴定。MSP(main spectrum profile)和PCA（Principal Component Analysis）分析为追踪弧菌感染的来源和发生率提供了理论支持。此外,特征峰和差异峰的发现对弧菌的鉴定也有帮助。     

南海西部风驱离岸急流次中尺度锋面的动力学分析

本文利用卫星观测资料和500 m分辨率数值模拟结果,结合理论分析,对南海西部夏季风场驱动的离岸急流海域次中尺度锋面及其不稳定对背景流场的动力学影响进行了研究。卫星观测和模拟结果表明,南海西部（WSCS）存在侧向尺度为O（1-10）km的次中尺度锋面,在地转和非地转运动的共同作用下,次中尺度密度锋面具有一阶Rossby（Ro）和Richardson（Ri）数。锋面诊断结果显示,沿锋面急流方向的风场强迫引起了显著的跨锋面Ekman净输送,有效地在跨锋面方向将表层冷水平流输送至暖水侧,导致海表浮力损失。减弱的垂向层结和增强的水平浮力梯度使得锋面海域出现负Ertel位涡（PV）,表明该密度锋面易受次中尺度对称不稳定（SI）的影响。次中尺度锋面不稳定引起的跨锋面次级环流能够显著增强垂向速度,其最大值可达100 m·d^-1。能量评估结果表明,次中尺度湍流的两个主要能量源,即地转剪切项（GSP）和垂向浮力通量（BFLUX）在锋面海域显著增强表明在沿锋面急流方向的风场强迫作用下,大尺度地转流的地转剪切动能和锋面有效位能能有效地通过锋面不稳定向次中尺度过程传递。因此,次中尺度锋面及其不稳定有助于增强局地垂向交换和正向串级地转能量,可以为夏季WSCS高叶绿素浓度的相干结构和锋面地转能量的正向传递提供新的动力解释。     

神光Ⅲ原型单端驱动黑腔等离子体聚心时间研究

在神光Ⅲ原型装置上,利用8束激光单端驱动半腔靶实验研究了黑腔等离子体聚心时间.通过改变腔底结构建立了4种黑腔辐射源,比较了等离子体径向的聚心时间与黑腔辐射温度的变化关系.径向聚心时间和辐射温度分别由X射线分幅相机和软X射线能谱仪给出.研究发现,4种辐射源的等离子体径向聚心时间与辐射温度之间存在正比关系,聚心时间随辐射温度升高而延后.     

Crossover from a pseudogap state to a superconducting state

This paper deduces that the particular electronic structure of cuprate superconductors confines Cooper pairs to be first formed in the antinodal region which is far from the Fermi surface, and these pairs are incoherent and result in the pseudogap state. With the change of doping or temperature, some pairs are formed in the nodal region which locates the Fermi surface, and these pairs are coherent and lead to superconductivity. Thus the coexistence of the pseudogap and the superconducting gap is explained when the two kinds of gaps are not all on the Fermi surface. It also shows that the symmetry of the pseudogap and the superconducting gap are determined by the electronic structure, and non-s wave symmetry gap favours the high-temperature superconductivity. Why the high-temperature superconductivity occurs in the metal region near the Mott metal-insulator transition is also explained.     

基于立体环形邻域的GMRF模型及其在遥感影像纹理识别中的应用

对传统的GMRF模型进行了扩展,建立了基于立体环形邻域的GMRF模型,并设计了分步模型参数求解方法。这种新的模型不仅全面考虑了多光谱高分辨率影像中各波段内像元之间的空间相关性,而且还顾及了波段间像元的相关性。与传统的GMRF模型相比,基于立体环形邻域的GMRF模型提取的纹理信息更为丰富。采用Forrest彩色纹理图像和QuickBird卫星遥感影像进行了实验验证,实验结果表明本文提出的基于立体环形邻域GM-RF模型的纹理识别算法具有较强的普遍适用性,对不同情况的纹理影像(即空间相关性主要存在于波段间还是波段内)进行识别均能获得较好的分类效果。     

基于JSCORS的江苏省现代大地坐标框架的建设与维护

本文结合江苏省现代大地坐标框架的建设和JSCORS的运行维护,通过对江苏省原大地坐标框架的情况分析,介绍了江苏大地坐标框架的建设方案.结合区域稳定基点组的确定、框架维护和复测机制的建立、框架维护系统的建设等内容,提出江苏省大地坐标框架维护的解决方案.     

基于小波变换的沿海滩涂LIDAR影像降噪研究

LIDAR 数据的精度受到多种因素影响,选择合适的模型优化LIDAR影像,分离LIDAR观测数据与噪声,可有效提高LIDAR数据精度.本文研究基于小波变换的LIDAR影像降噪模型,并将小渡降噪与各种常规方法进行对比分析,选择客观评价指标比较各方法的降噪效果,认为小波变换法更适合LIDAR影像降噪,能保留更多的有效信息.     

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Fine-grained Pleistocene deepwater turbidite channel system on the slope of Qiongdongnan Basin, northern South China Sea

Recently, as oil exploitation has become focused on deepwater slope areas, more multi-channel high resolution 2D and 3D seismic data were acquired in the deepwater part of the Qiongdongnan Basin, northern South China Sea. Based on 3D seismic data and coherence time slice, RMS and 3D visualization, a series of deepwater channels were recognized on the slope that probably developed in the late Quaternary period. These channels trend SW–NE to W–E and show bifurcations, levees, meander loops and avulsions. High Amplitude Reflections (HARs), typical for channel–levee complexes, are of only minor importance and were observed in one of the channel systems. Most of the detected channels are characterized by low-amplitude reflections, and so are different from the typical coarse-grained turbidite channels that had been discovered worldwide. The absence of well data in the study area made it difficult to determine the age and lithology of these channels. Using a neighboring drill hole and published data about such depositional systems worldwide, the lithology of these channels is likely to be dominated by mudstones with interbedded thin sandstones. These channels are formed by turbidity currents originated from the little scale mountain river of mid-Vietnam in SW direction and were probably accompanied by a relative sea level drop in the last glacial age. These channels discovered on the northern South China Sea slope are likely to be fine-grained, mud-dominant and low N:G deposits in a deepwater paleogeographic setting.     

The variations in optical properties of CDOM throughout an algal bloom event

For studies in bio-optical oceanography, visible light properties by classes of dissolved organic matter should be characterized. The regional adjustment of model parameters is one approach which is being widely used to refine bio-optical models. In the present study, buoy and laboratory data were acquired, and during the 15-day observation period an algal bloom event occurred. The absorption coefficient of CDOM at 443 nm, a_g(443), changed in the range of 0.09–0.35 m⁻¹ and 0.1–0.34 m⁻¹ for two depths, 0.32 m and 2.3 m., respectively, throughout the entire period. CDOM absorption was larger for bloom conditions than for non-bloom conditions. In addition, the fraction of CDOM in total absorption was higher during the bloom event than that of non-bloom conditions. The spectral slope of CDOM absorption, S_g, regressed over 400–500 nm, ranged from 0.015 to 0.0185 nm⁻¹ with an average of 0.0166 nm⁻¹. CDOM fluorescence intensity (Fcdom) was obtained using an internal Raman standard and varied over the range of 467.44–1538.23 in relative units. Variations in Fcdom showed a similar pattern to that of CDOM absorption. A robust non-linear relationship between Fcdom and CDOM absorption was found, with a correlation coefficient of 0.893, throughout the whole observation. An attempt to describe S_g with absorption showed a promising consequence that can be described with a negative correlation during the bloom, however, without any distinguishable tendency for non-bloom time span. A two-band ratio algorithm was also carried out for retrieving CDOM absorption. The variations in optical properties of CDOM may be related to the complicated environment in the estuarine waters, which may result from different sources of CDOM.