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Extensive genetic divergence among Diptychus maculatus populations in northwest China

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.     

Urban Metabolism Based on Emergy and Slack Based Model: A Case Study of Beijing,China

The key to studying urban sustainable development depends on quantifying stores, efficiencies of urban metabolisms and capturing urban metabolisms′ mechanisms. This paper builds up the metabolic emergy account and quantifies some important concepts of emergy stores. Emphasis is placed on the urban metabolic model based on the slack based model(SBM) method to measure urban metabolic efficiencies. Urban metabolic mechanisms are discussed by using the regression method. By integrating these models, this paper analyzes the urban metabolic development in Beijing from 2001 to 2010. We conclude that the metabolic emergy stores of Beijing increased significantly from 2001 to 2010, with the emergy imported accounting for most of the increase. The metabolic efficiencies in Beijing have improved since the 2008 Olympic Games. The population, economic growth, industrial structures, and environmental governance positively affect the overall urban metabolism, while the land expansion, urbanization and environmentally technical levels hinder the improving of urban metabolic efficiencies. The SBM metabolic method and the regression model based on the emergy analysis provide insights into the urban metabolic efficiencies and the mechanism. They can promote to integrate such concepts into their sustainability analyses and policy decisions.     

Nutrient effects of broodstocks on the larvae in Patinopecten yessoensis

Patinopecten yessoensis is a commercial valuable species. This study deals with the effect of nutrient effects of the broodstock(mainly ovaries) on the larvae. Concentrations of total carbohydrate, total protein and total lipid in the gonads of P. yessoensis from three Hatcheries(Hatchery 1, Hatchery 2, and Hatchery 3) were determined before and after spawning. The relationship between the nutrient concentration in ovaries before spawning(BC) and that of larvae(LC) was assessed as well as the change in nutrient levels in ovaries after spawning(DC). Results indicate that the BC of total carbohydrate(7.66%) and total lipid(14.48%) in ovaries were significantly higher than in testes(5.20%, 5.20% respectively), whereas the BC of total protein in the ovaries was lower(61.76%) than in the testes(81.67%). The different gonadal composition suggests the different nutrient demands between male and female broodstocks in breeding season. P atinopecten yessoensis gonads contained a higher proportion of lipids, in comparison to other bivalves, which might be a response to the low ambient water temperatures. Further analysis of fatty acids showed that the concentrations of n-3PUFA, EPA and DHA in larvae(LC) were positively correlated with BC and DC, indicating the significant nutrient influence of broodstocks on the larvae. As these fatty acids are important in metabolism, and have been demonstrated to be influential to the viability of the larvae, larval growth and the settlement, spat growth, and juvenile survival in many bivalves, they could possibly be used as indexes to evaluate, and predict condition of broodstocks and larvae.     

菏泽市气候干湿特征评估以及干湿风险区划研究

为研究34年来菏泽市气候干湿状况的变化,并对该市干旱灾害进行风险区划,给市、区(县)各级人民政府防御干旱灾害风险提供理论数据支撑,指导农、林业的生产和发展。主要利用菏泽市最近34年气象站基本资料,分析菏泽市的潜在蒸散量和湿润指数变化,同时利用地理区划软件Mapinfo对该地区的干湿风险灾害进行区划分析。结果表明:(1)20世纪80年代以来,菏泽市年均潜在蒸散量和降水量水平都有所增加,但降水量的增加程度远远低于潜在蒸散量,于是湿润指数一直呈现下降趋势,年均降幅约为1.05%;(2)年均潜在蒸散量的增加主要由于气温上升和湿度下降两个因素影响,其中湿度下降对其产生的影响最大,两者正相关系数分别为0.312和0.94;(3)从时间轴上看,每年10月到次年6月为菏泽市干旱灾害多发期,尤其是每年的12月到次年4月,月平均湿润指数<0.5,为旱灾易发期;(4)目前,鲁西南8县1区中,单县的湿润程度最好,曹县、定陶、牡丹区等次之,鄄城县的湿润程度最差。     

车辆路径问题:从时间地理学的视角

车辆路径问题具有典型的时空分布特征,受到众多时空约束条件的制约。在车辆路径规划中,综合考虑时间和空间因素是非常必要的。本文从时间地理学这一全新的视角来研究车辆路径问题,提出一套完整的时间地理学分析框架,阐述了时间地理学的基本概念,提出了车辆路径问题中的时空约束、时空路径、时空棱柱、时空可达性、时空距离等概念,并给出了图示或定量化的度量方法。论文提出的时空距离度量方法综合考虑了顾客在空间位置和时间窗口2个方面的特征,可更科学地判定顾客之间的"邻近性"。论文通过设计一种求解大规模软时间窗车辆路径问题的算法,证明了时空距离的价值,并展望了时间地理学在求解动态车辆路径规划问题、移动设施路径规划问题等方面的应用。本文的贡献在于,通过时间地理学所提供的一系列概念和方法,实现了在统一的框架下同时考虑车辆路径问题(VRP)的时间和空间特征的构想,挖掘了传统时间地理学理论在车辆路径领域中的应用潜力,这将有利于更快或者更好地求解VRP问题。     

西沙海域天然气水合物地震资料高密度双谱速度分析技术探讨

基于南海西沙海域天然气水合物二维高分辨率多道地震数据资料,使用Geovation地震资料处理系统的高密度双谱速度分析方法进行处理。在叠前时间偏移处理后的道集基础上,进行高密度双谱非双曲线型NMO自动拾取,最终获得解决各向异性问题后的叠前时间偏移剖面。分析结果表明,高密度双谱速度分析技术实现了速度逐道逐点的自动拾取,提高了时间方向的分辨能力、空间方向的分析密度,可以充分挖掘水合物地震数据的潜力。高密度双谱速度分析方法在提高处理质量的同时,可以帮助判定水合物的富集层位,研究似海底反射层附近的详细速度结构,为西沙海域水合物地震资料综合解释提供依据。     

20世纪南极菲尔德斯半岛毛皮海豹食谱比例变化的同位素混合模型比较分析

稳定同位素比值分析已成功用于示踪动物的食物来源研究,近年来已发展了多个同位素混合模型用于计算混合物中多来源物质的组成比例。本文以过去百年来南极菲尔德斯半岛南极毛皮海豹(Arctocephalus gazella)毛发的C、N同位素数据为基础,运用同位素混合模型中的欧几里得距离法、线性模型和贝叶斯模型进行计算、分析和比较,得到毛皮海豹食谱中南极磷虾(Euphausia superb)、南极电灯鱼(Electrona antarctica)和尼氏裸灯鱼(Gymnoscopelus nicholsi)所占比例随时间的变化趋势。各模型计算得出的结果可分为两组,分别是欧几里得距离法公式(1)和(2)的结果,表现为近百年来毛皮海豹食谱中Euphausia superb比例与其毛发稳定N同位素比值呈同步上升的趋势;第二组为欧几里得距离法公式(3)、线性模型和贝叶斯模型的结果,Euphausia superb比例与毛皮海豹毛稳定N同位素比值呈反相关关系。综合对比研究区域气候、海冰环境变化背景和已有的南极磷虾种群密度调查资料分析,第二组模型结果与实际情况较为相符,可用于讨论食物比例的时间变化趋势。近百年来,菲尔德斯半岛毛皮海豹食谱中Euphausia superb的比例显著下降,很可能是由于区域气候快速变暖和海冰的消退造成的。本文的计算结果指示西南极半岛气候变暖已显著影响到区域海洋食物链变化。     

新型多立柱式Spar平台涡激运动特性研究

通过数值模拟和模型试验方法对一新型的Spar平台——多立柱式Spar平台的涡激运动特性进行研究。平台硬舱为四根圆柱方形阵列布置加方形中心井的形式,各柱间会产生复杂的相互干扰,不同于常规的单立柱Spar平台或半潜式平台,因此对其开展相应的涡激运动研究具有重要意义。研究主要关注的是不同来流角、不同流速下平台的涡激运动特性。通过涡激运动时的横荡运动、水平面内运动轨迹、首摇运动和水动力系数及绕流流场的分析,可得:在折合速度约为6~8范围内,平台横荡运动存在明显的“锁定”现象(0°来流角无侧板时“锁定”范围约为Ur=7~10)。在0°来流角下较高流速时,减涡侧板对涡激运动有明显的抑制效果。平台的运动轨迹近似与平台硬舱截面的对角线平行(0°来流角除外),这不同于常规的单立柱Spar平台。此外,试验中还观察到较明显的首摇运动。涡激运动时各柱间绕流存在复杂的相互干扰,而在柱后形成共同的涡结构。