Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

青藏高原隆升的非线性动态有限元仿真研究

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。     

ECOLOGICAL SERIES OF SOIL ANIMALS IN DARLIDAI MOUNTAIN

The ecological series of soil animals under the broad-leaved and pine mixed forest in Darlidai Mountainwas studied. Seven sample plots were selected according to different altitude gradients, which belong to different vegeta-tion types. By investigating and analyzing soil animals in every sample plot it is found that there are 45 groups and 1956individuals, which axe involved in 3 phylums, 7 classes, 16 orders, respectively. The altitude is a key factor which af-fects ecological series of soil animals. Both the groups and individuals of soil animals increase with altitude increasingunder certain conditions, which contrastes with ordinary cases, resulting from special micro-climate in studied area. Thegroups and individuls of soil animals are the most under the broad-leaved and pine forest on the top of the mountain, andthe least under Picea-Abies forest in the foot of the mountain.     

大同—阳高Ms6.1地震前兆综合加权信息熵的变化

利用“地震预报计算机专家系统”的思想对大同 -阳高 Ms6 .1地震前每一前兆异常事件进行综合评估 ,以每一异常的最可能发震时间来计算发震概率 ,利用地震前兆综合加权信息熵研究了系统熵值与地震的关系。对华北地区的地震前兆综合加权信息熵研究表明 ,在大同 -阳高 Ms6 .1地震前 ,信息熵出现了明显的减熵有序变化     

对青藏东北缘现今块体划分、运动及变形的初步研究

利用2维非连续变形分析方法（DDA），以位移代替围压作为边界约束力，研究青藏东北缘现今块体划分及其运动变形。根据该地区地质构造及地震活动，以GPS点测量位移作为模拟结果约束点，得出了较合理的块体划分模型和随时间演化的主应变分布图，并把应变高值区与近几年来发生的5级以上地震作对比，得出了研究区内地震危险性可能较大的区域。另外，对模拟的甘青块体与阿拉善块体的边缘带断裂左旋运动做了大概计算。     

抽油机悬点投影测量仪

油田抽油机的抽油杆幌动幅度过大，是引起抽油杆断裂的一个重要因素，检测这种幌动幅度是防止抽油杆断裂的一种有效手段。设计了一种基于面阵CCD和普通半导体激光器（LD）测量这种幌动的悬点投影测量方法，通过数字卷积滤波，达到了范围为0－40mm，误差＜0.2mm的技术检测指标。     

Influence of the Choice of Core-Envelope Transition Point on the Binary Merger of Two Main-sequence Components

We have studied the influence of different choices of core-envelope transition point on the final merger of contact binaries with two main-sequence components. A binary of 1.00 + 0.90M⊙ with an initial orbital period of 0.35d is examined. The mass fraction of the primary mixed with the matter of the secondary, qmix, determined by the chosen core-envelope transition point, ranges from 0.04 to 1.00 in our analysis. If as qmix< 0.8, none of the     

Bacterial productivity in the Prydz Bay and its adjacent waters,Antarctic

1　IntroductionBacteriaandtheiractivitiesplayanimportantroleintheelementalbiogeochemicalcyclesandenergytransformingintheocean (Zhenetal.1 997) .DortchandPackard(1 989) proposedthatfoodwebsintheeutrophicwatersaredominatedbythebiomassofprimaryproducerswhilefoodwebsintheoligotrophicwatersaredominatedbythebiomassofmicrobes.Heterotrophicbacteriahadbeenshowntoplayanimportantroleinthedecompositionoflarge ,rapidlysinkingorganicparticleswithinandbelowtheeuphot iczone ,andfurthertoaffecttheelementaldyn…     

观驾山—黑岛一带信息量法找金研究

本文以成矿地质模型为依据，对观驾山—黑山岛一带的化探、物探、遥感及地质等信息变量进行了详细的分析，选取了１０个信息变量２４个标志状态；在此基础上，运用信息量法对研究区内４５８个单元的信息量值进行计算，并由此确定了研究范围内的成矿远景区。事实表明，研究区信息量成矿预测是非常有效的     

Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.