Primary molecular basis of androgenic gland endocrine sex regulation revealed by transcriptome analysis in Eriocheir sinensis

In crustaceans, the male sexual dif ferentiation and maintenance are specially regulated by androgenic gland(AG). However, little is known about the genes involved in the regulation process.RNA-Seq was performed on AG with ejaculatory duct(AG_ED) and ejaculatory duct(ED) as control in Eriocheir sinensis, one of the most important economic and ?shery crabs with typically sex dimorphism. A total of 925 unigenes were identi?ed as dif ferentially expressed genes(DEGs) and the expression of nine genes randomly selected was con?rmed by qRT-PCR. 667 unigenes were up-regulated in AG_ED, being supposed to be AG preferential genes. Among them, the full length of i nsulin-like androgenic gland factor( IAG) cDNA named as Es-IAG was obtained as a logo gene of AG, which together with the genes i nsulin-like receptor( INR), and s ingle insulin binding domain protein( SIBD), might constitute the sex regulation pathway. Several sex related genes were identi?ed, and their function will have to be investigated. Also,the identi?cation of j uvenile hormone epoxide hydrolase 1( JHEH1), ecdysteroid 22-hydroxylase( DIB) and e cdysone receptor( ECR) preliminarily clari?ed the molecular regulation mechanism of eyestalk-AG-testis axis, which plays important roles in molting and reproduction. The results will enhance our understanding for the molecular basis of the AG involved in male sex regulation in crabs.     

粒子加速器中高精度丝线绝对位置测量技术研究

粒子加速器中采用振动线技术进行共架单元上多块磁铁的高精度预准直,需要将振动线代表的磁中心引出进行共架单元之间的相对准直。为了获取以振动线为代表的丝线高精度空间绝对位置,本文对采用近景摄影测量技术进行点、线位置求解的方法进行了研究。利用单相机多站位的方法进行拍摄,通过标定板提供的高精度控制点坐标,采用自标定光束法平差对相机进行标定,并提出将空间直线离散成空间2点进行直线整体精确求解。对标定板上由2点组成的模拟直线进行测量验证,并与标定板提供的理论值进行对比,结果显示空间直线位置获取精度优于5 μm。这表明了本文理论和方法的正确性,为进一步研究丝线高精度空间绝对位置测量打下了基础。     

秦岭地区NDVI与地形因子的相关性分析

秦岭山区地形因子是影响植被分布的重要因素。选取2001、2009和2017年MODIS陆地产品MOD13Q1数据和DEM数据,从DEM中提取地形因子,高程、坡度和坡向,与MODIS的NDVI数据结合,分析了地形对秦岭地区植被空间分布影响。研究结果表明:(1)NDVI随着高程的增大而逐渐增大,在高程1800 m左右时达到最大值,随后又随着高程的增大而减小;(2)NDVI在坡度0°～5°间逐渐增大,在5°～40°呈稳定趋势,从40°开始缓慢减小,60°达到乔木能够生长的坡面倾角临界值,当坡面倾角大于60°时植被指数开始快速减小;(3)受太阳辐射的影响,坡向在NW 270°～360°,SE 240°～270°之间的植被长势较好,其余坡向上长势一般。     

京津冀协同治理的回顾与前瞻

京津冀区域治理是伴随三地协作发展的进程,从政府层面的管制转向各利益主体协调治理,从自上而下的竞争合作转向上下结合的协同合作的一系列有效应对举措。经过30多年的努力,学界和政府部门已经形成了较为丰富的京津冀区域治理研究成果。通过梳理自1984年以来相关区域的研究,尝试从时空维度厘清京津冀区域治理的研究脉络及其变化,剖析目前京津冀区域治理所涉及的治理模式、协调体系、以及形成的发展策略,为改进和提高京津冀区域治理效果提供参考。京津冀协同治理过程是学术界与政府的互动融合过程,其中政府的作用居主导地位。未来将在治理主体、治理机制、治理效果评价和文化、医疗、养老等领域的协调发展方面得到深化和细化。     

基于DEM分形特征的坡度尺度变换模型

利用DEM提取坡度具有明显的尺度依赖性,探求DEM在不同尺度下表现出的规律关系,建立多尺度变换模型,以实现不同尺度间的转换是地形分析研究的热点和难点。本文阐释了DEM表面与地表粗糙度分形维数值的地学意义及内在关系,并利用分形对象的自相似性原理,建立了一种基于DEM分形特征的坡度尺度变换模型。选取四川丘陵地区某小流域为研究区,进行坡度尺度变换实验和误差分析,结果表明该模型能有效实现坡度尺度变换:在非平坦地区(坡度>1°)一般重采样方法变换得到的坡度误差为该方法的1.86倍;从信息熵理论分析,经该方法转换后的坡度信息得到了显著恢复。对于无1︰1万及以上精度地形数据的西南山区,利用该方法获取高精度坡度数据具有重要的理论价值和现实意义。     

震区植被恢复初期土壤理化性质与土壤呼吸间的关系

为了解地震灾区不同恢复方式下土壤理化性质和土壤呼吸间的关系,选取2种典型气候区(干旱河谷气候区与亚热带季风气候区)为研究区,设置人工恢复、未受损、自然恢复3种恢复方式的固定样地,定期测定土壤呼吸与土壤理化性质(有机碳、全氮、全磷、速效氮、有效磷、容重、孔隙度、电导率和pH值),并分析各因子间的相关关系。结果表明:气候类型显著影响土壤全氮、容重、孔隙度、电导率以及土壤呼吸,表现为干旱河谷气候区显著优于亚热带季风气候区;恢复方式显著影响土壤呼吸速率、有机碳、全氮、全磷、速效氮、有效磷含量、C:P、N:P、容重、孔隙度和电导率,基本表现为未受损样地优于人工恢复样地优于自然恢复样地;二者交互作用显著影响土壤呼吸、土壤物理性质和除全磷、有效磷外主要土壤养分的流失。两种气候区土壤呼吸的影响因子不同,干旱河谷气候区主要为土壤有机质、全氮、全磷、速效氮、pH值、容重和孔隙度,亚热带季风气候区主要为土壤有机质、全氮、速效氮、容重和孔隙度。本研究为评价和优化西南地区灾后恢复治理措施提供一定科学依据,对其生态安全维护和生态屏障建设提供帮助。     

条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。     

一种顾及有色噪声的四星座GNSS动态导航滤波算法

经典Kalman滤波要求噪声是高斯白噪声,而动态GNSS定位的观测误差和状态预测误差往往是有色噪声。本文提出一种简便的有色噪声函数模型拟合滤波算法,采用前面历元的观测残差和状态残差建立有色噪声模型,削弱有色噪声对动态导航解算的影响。采用四星座GNSS接收机观测数据进行动态导航实验,结果表明,顾及有色噪声的动态导航滤波算法比未顾及有色噪声的经典Kalman滤波算法定位精度更高,三维位置精度提升9%以上。     

Effect of carbon and nitrogen ratio control on Artemia growth,water quality,biofloc microbial diversity under high salinity and zero-water exchange culture condition

Biofloc technology has been applied successfully in the intensive aquaculture of several fish and shrimp species.The growth of heterotrophic microorganisms can be stimulated through adding extra carbon,which reduces the nitrogen level in the water and provides microbial protein to the animals.However,most of the studies and practical applications have been conducted in freshwater and marine environment.This paper focused on brine shrimp Artemia that lives in high salinity environment together with other halophilic or halotolerant microorganisms.The effect of carbon supplementation on Artemia growth,water quality,and microbial diversity of bioflocs was studied in the closed culture condition without any water exchange.The salinity of the culture medium was 100.A 24-d culture trial was conducted through supplementing sucrose at carbon/nitrogen(C/N)ratio of 5,15,and 30(Su5,Sul5,and Su30),respectively.The culture without adding sucrose was used as a control.Artemia was fed formulated feed at a feeding ration of 60% recommended feeding level.The results showed that sucrose supplementation at higher C/N ratio(15 and 30)significantly improved the Artemia survival,growth and water quality(P0.05).Addition of sucrose at C/N ratio of 15 and 30 significantly increased biofloc volume(BFV)(P0.05).The Illumina MiSeq sequencing analysis showed that supplementing carbon at C/N ratio of 15 had a better total bacterial diversity and richness,and shaped the microbial composition at genera level.This study should provide information for studying the mechanism of biofloc technology and its application in high salinity culture conditions.     

Impacts of Coastal Reclamation on Natural Wetlands in Large River Deltas in China

Little information is available on the impacts of coastal reclamation on wetland loss in large-river deltas at a regional scale.Using remote sensing data of coastal wetland and reclamation in four deltas in China from 1978 to 2014, we tracked their continuous area changes in four periods: 1978–1990, 1990–2000, 2000–2008, and 2008–2014. The areal relation between wetland loss and reclamation was quantified and used to identify coastal reclamation mode intensity coupled with another three indicators: reclamation rate,accretion rate and land-use intensity of coastal reclamation. The results showed that coastal reclamation driven by economic development reduced, or even reverse the original growth of delta which was determined by the offset between wetland acceleration rate and wetland loss rate. Generally, the area of reclamation showed a positive linear correlation with the area of wetland loss. The findings imply that human activities should control reclamation rate and intensity to alleviate total wetland loss and maintain wetland ’net gain’.Inappropriate coastal reclamation modes can magnify total wetland loss;therefore, coastal reclamation with a slow increment rate and low impervious surface percent is of great importance for sustainable development in future coastal management.