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51.
鳗弧菌(Vibrio anguillarum)M3菌株生长条件及其对蛋白酶产量的影响 总被引:2,自引:5,他引:2
采用体外测定细菌浓度、胞外产物(ECP)蛋白含量和蛋白酶活力的方法,进行了鳗弧菌M3菌株在2216E培养基中的培养条件研究。结果表明,该菌株用固体培养基培养至24h左右,可得到较高的菌体浓度、ECP蛋白含量和蛋白酶活力。采用响应面分析方法设计实验,用SAS统计软件分析数据,得到NaCl浓度、pH值和温度对菌体生长及蛋白酶产量影响的回归模型。在2216E培养基的基础上,添加不同氮源、碳源物质以及不同浓度蛋白胨进行生长研究。结果表明,胰大豆蛋白胨能促进菌体生长及ECP蛋白分泌;NH4Cl与酪蛋白水解物可抑制蛋白酶的产生;牙鲆肌肉匀浆对菌体、ECP蛋白产量和蛋白酶产生有不同程度的促进作用;培养基中蛋白胨浓度为4%时菌体量与ECP蛋白含量达最高值,在蛋白胨浓度为2%时蛋白酶分泌量已稳定;1%的葡萄糖、蔗糖、甘油均能显著地提高菌体及ECP蛋白产量,却抑制了蛋白酶的产生。 相似文献
52.
将大气和海洋中f-平面上中尺度地形的扰动问题统一起来作为一个地球物理流体力学问题,应用涡度、能量和经圈动量守恒条件,将原来高度非线性方程变成一个二阶椭园型非线性方程,用数值模拟方法分析了大气中的过山运动和海洋中的沿岸上升流,给出了与观测接近的模拟结果以及运动过程对内部物理参数和外界条件的敏感性分析。所提方法可供进一步的动力学分析和数值研究参考。 相似文献
53.
南海南部晚中新世的放射虫及其环境探讨 总被引:2,自引:0,他引:2
南海是东亚古季风产物的主要沉积盆地,保存着比陆地更加完整和连续的沉积记录。详细分析了ODP1143号钻井晚中新世的放射虫化石群,主要根据标志种Diartus petterssoni,D.hughesi和Stichocorys delmontensis等的分布特征,分别建立了南海南部晚中新世的RN6,RN7和RN8等3个放射虫化石带,并讨论了其地层年龄。探讨了以地层中放射虫的丰度变化特征等在南海南部作为东亚古夏季风活动替代性指标的可能性,初步说明东亚古夏季风可能早于8.7Ma B.P.出现,约在8.24Ma B.P.强化达到高峰,认为与印度季风的出现几乎同步或略早。 相似文献
54.
Seasonal and annual variations of marine sinking particulate flux during 1993~1996 in the central South China Sea 总被引:1,自引:0,他引:1
CHEN Ronghu WIESNER M G ZHENG Yulong CHENG Xinrong JIN Haiyan ZHAO Qingying ZHENG Lianfu 《海洋学报(英文版)》2007,26(3):33-43
A total of 67 samples from the upper and lower sediment traps in the central South China Sea were analyzed, which were collected during 1993~1996. It is indicated that the distribution of stable isotope values, surface primary productivity, fluxes of total particulate matter, carbonate, biogenic opal, organic carbon, planktonic foraminiferal species and their total amount exhibit obviously seasonal and annual fluctuations. High values of the fluxes occurred in the prevailing periods of the northeastern and southwestern monsoons, and the low values occurred during the periods between the two monsoons. The fluxes of some planktonic foraminiferal species (Globigerinoides sacculifer, G. ruber, Globigerinita glutinata, Neogloboquadrina dutertrei) and their percentages also exhibit two prominent peaks during the prevailing periods of the northeastern and southwestern monsoons respectively, while those of Globigerina bulloides, Globorotalia menardii and Pulleniatina obliquiloculata only exhibit one peak in the prevailing periods of the northeastern monsoon. In addition, fluxes and percentages of Globigerinoides sacculifer and Globorotalia menardii as well as the fluxes of carbonate and total amount of planktonic foraminifera decrease gradually from 1993 to 1996, and those of Globigerina bulloides, Globigerinita glutinata and biogenic opal increase gradually from 1993 to 1996. The fluxes of carbonate and organic carbon in the upper trap are higher than those in the lower one. The study indicates that the seasonal and annual variations of the sediment fluxes and planktonic foraminiferal species are mainly controlled by the changes of surface primary productivity and hydrological conditions related to the East Asian monsoon. The lower carbonate and organic carbon fluxes in the lower trap are related to the dissolution. 相似文献
55.
56.
以p-硝基苯酚-β-D-氨基葡萄糖苷(pNP-NAG)为底物,研究产物类似物:p-羧基苯酚和苯酚对青蟹N-乙酰-β-D-氨基葡萄糖苷酶(NAGase,Ec3.2.1.52)活力的影响.结果表明:p-羧基苯酚和苯酚对该酶有抑制的作用,IC50分别为20.0和100.0mmol/dm^3.p-羧基苯酚和苯酚对酶的抑制均表现为竞争性类型,其抑制常数K1分别为5.03和25.67mmol/dm^3.结合产物N-乙酰-β-D-氨基葡萄糖(NAG)抑制作用表现为反竞争性类型,判断NAGase水解pNP-NAG反应为有序双双反应(Ordered Bi Bi). 相似文献
57.
More and more researches show that neither the critical downward acceleration nor the critical slope of water waves is a universal constant. On the contrary, they vary with particular wave conditions. This fact moders the models either for the probability of wave breaking B or for the whitecap coverage W based on these criteria difficult to apply. In this paper and the one which follows we seek to develop models for the prediction of both B and W based on the kinematical criterion. First, several joint probabihstic distribution functions (PDFs) of wave characteristics are derived, based on which the breaking properties B and W are estimated. The estimation is made on the assumption that a wave breaks ff the horizontal velocity of water particles at its crest exceeds the local wave celerity, and whitecapping occurs in regions of fluid where water particles travel faster than the waves. The consequent B and W depend on wave spectral moments of orders 0 to 4.Then the JONSWAP spectrum is used to represent the fetch-limited sea waves in deep water, so as to relate the probahility of wave breaking and the whitecap coverage with wind parameters. To this end, the time-averaging technique proposed by Glazman (1986) is applied to the estimation of the spectral moments involved, and furthermore, the theoretical models are compared with available observations collected from published literature. From the comparison, the averaging time scale is determined. The final models show that the probability of wave breaking as well as the whitecap coverage depends on the dimensionless fetch. The agreement between these models and the database is reasonable. 相似文献
58.
CHEN Yan KE Caihuan ZHOU Shiqiang LI FuxueDepartment of Oceanography Key Laboratory for Marine Environmental Science of the Ministry of Education Xiamen University Xiamen China 《海洋学报(英文版)》2004,(3)
The development of embryos and larvae of Babylonia formosae habei living along the southeast coast of China is observed under laboratory conditions. The egg masses are laid by females on hard substrate at night and each capsule contains 100-500 eggs. Each egg is 250-280 mm in diameter. The first two cleavages of the embryo are meridional and equal, and a polar lobe is produced. Larval kidney, which only consists of a single cell, appears during the gastrula stage on each side of the embryo. The right tentacle develops prior to the left one. At 25-27℃, an intracapsulate veliger stage is reached about 4.5 d after deposition. The larvae hatch on the fifth day as swimming veligers with a shell length of 360 -500 mm. The newly hatched larva can ingest suspended algal cells from the water column and remains in the pelagic stage for 8-10 d. The newly settled juveniles are 900-1 200 mm in shell length. 相似文献
59.
辽河盆地大民屯凹陷流体压力特征 总被引:1,自引:0,他引:1
大民屯凹陷是辽河断陷内4个下第三系凹陷之一。在综合利用钻井、试井及地震等资料的基础上,系统研究并论述了大民屯凹陷流体压力特征。基于57口井的声波测井资料,凹陷内泥岩压力特征可区分为正常压力、异常压力或强超压等类型;根据152口井391个点的压力测试数据,凹陷内产油层段的压力梯度多接近于1;利用公式法模拟计算了47条地震剖面的流体压力、剩余压力及压力系数的分布特征,凹陷内剖面压力系统自上而下一般由正常压力、弱超压和强超压3部分组成。此外,还根据流体压力演化的基本原理及钻井、岩性与试井等实际资料,模拟恢复了大民屯凹陷的压力演化史,其可划分为超压原始积累、超压部分释放及超压再积聚3个阶段。总体上,大民屯凹陷的超压强度低于渤海湾盆地其他地区的超压强度。 相似文献
60.