深海细菌Pseudomonas sp. IOFA1中甲醛歧化酶FM11的纯化及性质研究

从印度洋深海沉积物中分离出一株能够高效降解甲醛的假单胞菌(Pseudomonas sp.IOFA1),该菌株可在40 min内完全降解100 mg/cm~3的甲醛.通过阴离子交换柱层析对菌株破碎液中的粗酶液进行纯化,得到一条具有甲醛降解活性的单一蛋白条带.经MALDI-TOF/TOF质谱鉴定,该蛋白为甲醛歧化酶蛋白(FM11).序列分析表明FM11与其它物种已知醛脱氢酶的氨基酸序列最高相似性为65%;结构域分析表明FM11含有2个保守的锌结合位点和一个NAD结合位点.酶学性质分析结果显示,FM11的最适反应温度为40℃,在25~50℃范围内能够保留95%以上的酶活力,尤其是在高于70℃的高温环境下仍保有约50%的活力,表现出广泛的温度适应性.FM11具有非常广泛的最适反应pH值范围(pH值为5~9),且在pH值4和pH值10时仍分别保留50%和80%左右的酶活力,表现出广泛的pH适应性.在pH值为5~7的条件下孵育1 h后,FM11仍保有97%以上的酶活,且在pH值为7~10条件下孵育1 h后,仍保留90%以上的酶活,表现出极好的酸性和碱性稳定性.Ca~(2+)、Mg~(2+)在低浓度时(0.5 mmol/dm~3)对FM11有较明显的激活作用.这些研究为我们进一步开发Pseudomonas sp.IOFA1甲醛歧化酶在降解甲醛方面的用途奠定了基础.     

基于高分辨率高度计沿轨数据吕宋海峡亚中尺度过程季节波动分析

亚中尺度过程是指海洋流场罗斯贝数接近于1,空间尺度量级1km、时间尺度量级1d的动力学过程.根据高分辨率的Jason-2卫星高度计沿轨数据,采用改进的方差归一化方法,结合一维功率谱斜率分析,对南海吕宋海峡海域亚中尺度过程进行季节性统计,结果表明:吕宋海峡亚中尺度过程季节高发月份特性与相应的海平面异常波动特性较为一致性,较大的月份主要出现在秋冬季节,夏季波动相对较弱.结合当前研究和实例观测,冬季黑潮入侵和两侧"波动高能区"涡流波动是亚中尺度高发的诱因.     

新疆乌鲁木齐东部早二叠世枕状玄武岩火山岩系的发现及大地构造意义

新疆乌鲁木齐东部野生动物园附近的下二叠统下部发育了一套以枕状玄武岩为代表的火山岩系。该玄武岩在公路上发育有两层,上部一层较厚,可达7～8m,下部一层2～3m。岩枕近圆形、肾状、枕状、条带状或蠕虫状等,多呈顶突底凹。岩枕长轴平行排列,长轴基本顺层面分布;有的岩枕中含大量海百合茎、珊瑚及腕足等生物化石。在TAS图中大部分样品位于玄武粗安岩,而在K_2O-SiO_2图上主要落在低K拉斑玄武岩区间。MnOTiO_2-P_2O_5图解显示以岛弧为主,常量元素的特征总体上更接近岛弧玄武岩。在Hf/3-Th-Ta图解上也以岛弧为主;在Zr/4-2Nb-Y图解显示以火山弧为主,常量元素的特点总体上更接近岛弧玄武岩。微量元素原始地幔标准化蛛网图表明为同源岩浆产物,具高度相似的演化过程,多种微量元素判别图揭示岛弧-弧后盆地环境;稀土总量明显较低,在稀土元素球粒陨石标准化图解上,其稀土分布曲线一致性较好,呈轻稀土富集右倾型,Eu为负异常,揭示了乌东玄武岩岩浆有一定分异。稀土元素配分曲线与弧后盆地玄武岩(BABB)具有很好的一致性。U-Pb和谐年龄为283±8Ma,结合地层及古生物资料推测乌东玄武岩喷发的时间为早二叠世早期。乌东枕状玄武岩-灰岩之下有一套(磨拉石)底砾岩,初定为石炭-二叠系的界限。底砾岩之下为下石炭统的中厚层灰岩,具有明显的喀斯特化,为不整合接触,揭示在两者之间发生了造山作用。通过对新疆乌东早二叠世早期的枕状玄武岩的地质特征、地球化学特征、形成环境和时代的研究,表明乌东一带早二叠世主要为一个岛弧和弧后盆地环境,进一步揭示了北天山北缘石炭-二叠世碰撞闭合造山之后又发生了松弛扩张形成了弧后盆地,海水再度大规模入侵。最终的闭合碰撞造山的时间最早可能在晚二叠世。由于乌东枕状玄武岩在喷出地表后受到了生物化石和陆源碎屑的污染,再加上侵入过程中地壳的污染,使其具有非常复杂的地球化学特点和多解性。乌东玄武岩的研究对于了解博格达山、甚至天山中段在晚古生代的构造沉积演化及造山作用具有重要意义,同时对准噶尔盆地、吐哈盆地及三塘湖盆地油气资源的形成与分布具有重要意义。     

江西牛形坝-柳木坑金银铅锌多金属矿床铋矿物类型及指示意义

江西银坑矿田的牛形坝-柳木坑矿床是赣南于都-赣县矿集区典型的金银铅锌多金属矿床。根据野外调研、系统矿相学研究和电子探针成分分析,表明铋在牛形坝-柳木坑矿床中是一种重要的伴生矿化金属元素,呈独立矿物或呈微细矿物包体分布在早期硫化物内部或边缘。铋矿物种类丰富,主要包括块硫铋银矿、硫铋银矿、板硫铋铜铅矿、针硫铋铅矿、硫铜铋矿、库辉铋铜铅矿、辉碲铋矿等。与同一矿集区石英脉型盘古山钨铋矿床相比,两矿床铋矿物种类均出现Pb-Ag-Bi-S、Te-Bi-S元素组合,赋存形式和含量有所不同,Cu-Pb-Bi-S元素组合仅在牛形坝-柳木坑矿床出现。矿床属于Au-Bi-(低Te)-S体系,铋盐矿物形成于中低温、中高密度、低盐度的成矿环境。从岩浆中分馏出来的成矿流体发生减压沸腾迫使大量蒸汽相逸散,硫逸度增加,铋主要以硫络合物形式运移,随着黄铁矿等硫化物沉淀,硫被大量消耗形成金矿物和碲化物(碲银矿)。成矿流体演化过程中由于温度和硫逸度进一步降低导致在成矿后期沉淀出大量铋硫盐矿物。成矿过程中的Bi促进金的迁移富集。铋矿物在两矿床中的赋存特征、化学成分、形成环境以及沉淀机制等表现出差异,说明铋具有成矿选择性,起到对成矿物质示踪的作用。     

内蒙古北山额勒根乌兰乌拉一带土壤测量地球化学特征及找矿方向

研究区位于内蒙古北山北带,成矿地质条件优越,1∶20万水系沉积物测量异常明显,且分布有额勒根乌兰乌拉斑岩型钼(铜)矿。以1∶5万土壤地球化学测量成果为依据,以地质认识为基础,研究了区内元素地球化学数据特征、地球化学场特征及综合异常特征。认为区内主成矿元素为Mo、Cu、Au,主要的控矿层位为咸水湖组火山岩段,成矿有利侵入体为石炭纪花岗闪长岩。划分出5种综合异常类型,其中与斑岩钼(铜)矿系统有关的综合异常和与奥陶系建造有关的综合异常是今后解剖找矿的重点。     

New Zircon U‐Pb Age of Granodiorite in Chifeng at the Northern Margin of North China Craton and Constraints on Plate Tectonic Evolution

正Objective The North China Craton(NCC)is one of the oldest cratons in the world.The accretionary belt at its northern margin has been the focus of scholars both at home and abroad(Zhu Junbing and Ren Jishun,2017).In recent years,a series of Late Paleozoic–Mesozoic intrusions trending E–W have been discovered within the northern margin of the NCC,forming a magmatic belt.The study     

Petrological and Os Isotopic Characteristics of Zedong Peridotites in the Eastern Yarlung–Zangbo Suture in Tibet

The Zedong ophiolites in the eastern Yarlung–Zangbo suture zone of Tibet represent a mantle slice of more than 45 km~2. This massif consists mainly of mantle peridotites, with lesser gabbros, diabases and volcanic rocks. The mantle peridotites are mostly harzburgite, lherzolite; a few dike-like bodies of dunite are also present. Mineral structures show that the peridotites experienced plastic deformation and partial melting. Olivine(Fo89.7–91.2), orthopyroxene(En_(88–92)), clinopyroxene(En_(45–49) Wo_(47–51) Fs_(2–4)) and spinel [Mg~#=100×Mg/(Mg+Fe)]=49.1–70.7; Cr~#=(100×Cr/(Cr+Al)=18.8–76.5] are the major minerals. The degree of partial melting of mantle peridotites is 10%–40%, indicating that the Zedong mantle peridotites may experience a multi–stage process. The peridotites are characterized by depleted major element compositions and low REE content(0.08–0.62 ppm). Their "spoon–shaped" primitive–mantle normalized REE patterns with(La/Sm)_N being 0.50–6.00 indicate that the Zedong ultramafic rocks belong to depleted residual mantle rocks. The PGE content of Zedong peridotites(18.19–50.74 ppb) is similar with primary mantle with Pd/Ir being 0.54–0.60 and Pt/Pd being 1.09–1.66. The Zedong peridotites have variable, unradiogenic Os isotopic compositions with ~(187)Os/~(188)Os=0.1228 to 0.1282. A corollary to this interpretation is that the convecting upper mantle is heterogeneous in Os isotopes. All data of the Zedong peridotites suggest that they formed originally at a mid-ocean ridge(MOR) and were later modified in supra–subduction zone(SSZ) environment.     

New Ar‐Ar Isotopic Ages of the Southern Tianshan Mountains,Kyrgyzstan and their Geological Implications

正Objective The Late Paleozoic Southern Tianshan Ocean is usually considered to be the last-closed ocean in the Tianshan Orogeny.However,there is still no consensus if this is indeed the case.Blueschist,eclogite and ophiolite are present in the Atbashi Range,Kyrgyzstan,which are believed to be the relics of the Southern Tianshan Ocean.New data obtained through Ar-Ar isotopic analysis in this     

Mixing of Enriched Lithospheric Mantle-Derived and Crustal Magmas: Evidence from the Habo Cenozoic Porphyry in Western Yunnan

New zircon U–Pb ages, whole-rock geochemistry and zircon Hf isotopes from the Habo porphyry Western Yunnan, China, were determined to provide constraints on the timing of uplift of the Eastern Tibetan Plateau. The intrusive rocks consist of shoshonitic porphyry (syenite porphyry and monzonite porphyry). Zircon laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) U–Pb dating indicates coeval emplacement ages of ~35 Ma. The porphyries have alkaline affinities, enrichment in large ion lithophile elements (LILEs) and light rare earth elements (LREEs) (e.g., Rb, Th, U, Pb), with depletion of high field strength elements (HFSEs) (e.g., Nb, Ti, Ta) and weak Eu anomalies. They display uniform Lu–Hf isotopic compositions with negative zircon εHf(t) values ranging from -3.9 to -0.6. The chemical characteristics of the syenite porphyries indicated that they most likely originated from the lower crust, with mantle-derived material involved in their generation. Geochemically, the monzonite porphyries are similar to the syenite porphyries; however, the lower MgO contents suggest that they were produced by different degrees of partial melting of the same lower crust source. Combined with the geochemical and isotopic data in this paper, imply that the alkali-rich porphyries of the Habo polymetallic deposit were derived from the partial melting of lower crust, enriched by mantle magma, formed in a conversion stage from stress extrusion (a strike-slip shear process) to local stress relaxation (a strike-slip pull-apart process) at the Ailaoshao tectonic zone.