Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

Detection of a high-confidence quasi-periodic oscillation in radio light curve of the high redshift FSRQ PKS J0805–0111

In this work,we have searched for quasi-periodic oscillations(QPOs)in the 15 GHz light curve of the FSRQ PKS J0805-0111 monitored by the Owens Valley Radio Observatory(OVRO)40 m telescope during the period from 2008 January 9 to 2019 May 9,using the weighted wavelet Z-transform(WWZ)and the Lomb-Scargle Periodogram(LSP)techniques.This is the first time to search for a periodic radio signal in the FSRQ PKS J0805-0111 by these two methods.Both methods consistently reveal a repeating signal with a periodicity of 3.38±0.8 yr(>99.7%confidence level).In order to determine the significance of the periods,the false alarm probability method was applied,and a large number of Monte Carlo simulations were performed.As possible explanations,we discuss a number of scenarios including the thermal instability of thin disks scenario,the spiral jet scenario and the binary supermassive black hole scenario.We expect that the binary black hole scenario,where the QPO is caused by the precession of binary black holes,is the most likely explanation.FSRQ PKS J0805-0111 thus could be a good binary black hole candidate.In the binary black hole scenario,the distance between the primary black hole and the secondary black hole is about 1.71×10¹⁶ cm.     

Attribution of trends in meteorological drought during 1960–2016 over the Loess Plateau,China

This study uses two forms of the Palmer Drought Severity Index(PDSI), namely the PDSI_TH(potential evapotranspiration estimated-by the Thornthwaite equation) and the PDSI_PM(potential evapotranspiration estimated by the FAO Penman-Monteith equation), to characterize the meteorological drought trends during 1960–2016 in the Loess Plateau(LP) and its four subregions. By designing a series of numerical experiments, we mainly investigated various climatic factors' contributions to the drought trends at annual, summer, and autumn time scales. Overall, the drying trend in the PDSI_TH is much larger than that in the PDSI_PM. The former is more sensitive to air temperature than precipitation, while the latter is the most sensitive to precipitation among all meteorological factors. Increasing temperature results in a decreasing trend(drying) in the PDSI_TH, which is further aggravated by decreasing precipitation, jointly leading to a relatively severe drying trend. For the PDSI_PM that considers more comprehensive climatic factors, the drying trend is partly counteracted by the declining wind speed and solar radiation. Therefore, the PDSI_PM ultimately shows a much smaller drying trend in the past decades.     

宁阳县蒋集镇水库土坝非稳定性探析

宁阳县蒋集镇地处汶河南岸,黄淮分水岭北侧,最大海拔高程361m.全镇区域面积12．7万km^2,其中山区、丘陵占2／3,岩石类型以花岗片麻岩为主。20世纪50-60年代兴建了117座水库塘坝,其中小（一）型水库2座,小（二）型水库15座,塘坝100座,总库容1700余万m^2,坝体以土坝为主。由于当时施工机械落后,技术有限,经费不足,造成工程质量较低,因而不少土坝产生渗流、流土、管涌、滑坡等现象。     

茯苓酸调节AMPK/GSK-3β/Nrf2信号通路对子宫内膜异位症大鼠内膜基质细胞铁死亡的影响

目的：探索茯苓酸调节腺苷酸活化蛋白激酶（AMPK）/糖原合成酶激酶3β（GSK-3β）/核因子E2相关因子2（Nrf2）信号通路对子宫内膜异位症（EM）大鼠基质细胞铁死亡的影响。方法：将51只SD大鼠于腹腔内异位移植子宫内膜组织构建EM大鼠模型,并将其随机分为模型组、茯苓酸低剂量（3.5 mg/kg）组、茯苓酸高剂量（7.0 mg/kg）组、茯苓酸高剂量（7.0 mg/kg）+盐酸多索啡（0.2 mg/kg）组,每组各12只,另取12只SD大鼠只开腹不移植,设为假手术组,药物分组处理后测定各组大鼠异位子宫内膜体积、异位子宫内膜组织铁死亡指标［谷胱甘肽（GSH）、丙二醛（MDA）水平、铁含量］、血清及异位子宫内膜组织炎症因子［肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）］水平、异位子宫内膜组织铁死亡相关蛋白［长链脂酰辅酶A合成酶4（ACSL4）、环加氧酶2（PTGS2）］与AMPK/GSK-3β/Nrf2通路相关蛋白表达。原代培养EM大鼠子宫内膜基质细胞,并将其随机分为对照组、茯苓酸低浓度（40 mg/L）组、茯苓酸高浓度（80 mg/L）组、茯苓酸高浓度（80 mg/L）+盐酸多索啡（10 μmol/L）组,以药物分组处理后,以CCK-8法、流式细胞术分别测定各组细胞增殖及凋亡;以试剂盒检测各组细胞铁死亡指标GSH及MDA水平、铁含量;以免疫印迹实验检测各组细胞铁死亡相关蛋白及AMPK/GSK-3β/Nrf2通路相关蛋白表达。结果：与假手术组相比,模型组大鼠异位子宫内膜体积、MDA水平及铁含量、血清及异位子宫内膜组织TNF-α及IL-6水平、ACSL4及PTGS2蛋白表达升高（P<0.05）,GSH水平、p-AMPK/AMPK、p-GSK-3β/GSK-3β及Nrf2蛋白表达降低（P<0.05）;与模型组相比,茯苓酸低、高剂量组大鼠异位子宫内膜体积、MDA水平及铁含量、血清及异位子宫内膜组织TNF-α及IL-6水平、ACSL4及PTGS2蛋白表达降低（P<0.05）,GSH水平、p-AMPK/AMPK、p-GSK-3β/GSK-3β及Nrf2蛋白表达升高（P<0.05）,且高剂量茯苓酸作用更强;盐酸多索啡可减弱高剂量茯苓酸对EM模型大鼠的作用。与对照组相比,茯苓酸低、高浓度组大鼠子宫内膜基质细胞活力、MDA水平及铁含量、ACSL4及PTGS2蛋白表达降低（P<0.05）,凋亡率、GSH水平、p-AMPK/AMPK、p-GSK-3β/GSK-3β及Nrf2蛋白表达升高（P<0.05）,且高浓度茯苓酸作用更强;盐酸多索啡可减弱高剂量茯苓酸对EM大鼠内膜基质细胞的作用。结论：茯苓酸可减轻EM大鼠炎症及铁死亡,抑制其子宫内膜基质细胞增殖及异位内膜生长,其作用机制可能是通过激活AMPK/GSK-3β/Nrf2信号通路来实现的。     

锶矿X

研究了找锶矿的     

闽西北铅锌矿成矿特征及控矿条件浅析

闽北地区属华南褶皱系的一部分,其中铅、锌为区内优势矿种,广泛分布于前震旦纪变质岩系中,尤其以前震旦纪龙北溪组的黑云斜长变粒岩、云母片岩、石英片岩中最为多见。其成矿特征可分为热液型铅锌矿床、充填交代型铅锌矿床及沉积变质热液叠加型铅锌矿床。其形成受地层、岩浆、构造（以断裂构造为主）控制较为明显,前震旦系变质岩系中的绿片岩、压扭性断裂及前震旦系变质岩系与岩体的接触带及其附近应是本区铅锌矿床找矿的主要方向。     

水库流域体积降水量预报方法研究

根据广东飞来峡水库流域汇流特征,将全流域划分为5个支流,并采用求积仪分别计算各支流面积;然后用加权平均法计算各支流的面雨量,并将各支流面雨量对相应的支流面积进行积分,计算各支流体积降雨量;利用洪水过程飞来峡水库入库流量、出库流量和水库流域蒸发量对体积降水量预报结果进行验证,求出误差订正系数.结果表明:利用加权平均法计算的水库流域体积降水量,经过误差订正后,预报准确率可控制在90%左右,基本满足预报业务服务要求.     

四川拉拉铁氧化物铜金矿床（IOCG）形成的矿相学证据

四川拉拉铜矿是我国西南重要的大型铜矿,并且共（伴）生丰富的金—钼—钴—稀土—铁可供综合利用。系统的矿相学研究表明,矿石矿物主要为热液磁铁矿、黄铜矿、黄铁矿、白铁矿和辉钼矿;矿床围岩蚀变种类较多,主要有黑云母化、硅化、碳酸盐化、钠长石化、钾长石化、磷灰石化、阳起石化、萤石化等;矿石结构包括自形- 半自形- 它形晶粒结构、交代残余结构、包含结构和枝状结构,矿石构造包括浸染状构造、条带状构造、脉状- 网脉状构造和角砾状构造;载铜矿物主要为黄铜矿,钴主要赋存于黄铁矿和白铁矿中,钼赋存于辉钼矿中,金主要以自然金或含银自然金形式赋存于黄铜矿、黄铁矿和叶碲铋矿中;磁铁矿中钛含量低,矿石中硫含量低,罕见铅锌硫化物矿物。因此,拉拉铜矿应属于典型的铁氧化物铜金矿床。     

郑州市大雾气候特点及一次个例分析

为了了解近20 a郑州市大雾的时空变化特征,根据1980-2005年郑州市大雾观测记录,统计分析了这一时期郑州市大雾的变化特点及产生大雾时的气象要素特征;并利用河南省地面和探空观测资料以及NCEP1°×1°再分析资料,从要素场、大气稳定度等方面,对2007年12月25-27日郑州市大雾形成原因进行了分析.结果表明:郑州市年雾日数呈下降趋势,每月都可以出现大雾,但12月最多,6月最少.降水后较大的相对湿度、稳定的大气层结以及近地层较小风速等是大雾形成的必要条件.