Isolation and characterization of bioactive fungi from shark <Emphasis Type="Italic">Carcharodon carcharias</Emphasis>’ gill with biopharmaceutical prospects

Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark (Carcharodon carcharias). A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated significant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit (ITS-LSU) rDNA-sequence BLAST comparison. Four genera, Penicillium, Aspergillus, Mucor, and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profiles. These results detail the broad range of bioactive fungi found in a shark’s gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the first study characterizing shark gill fungi and their bioactivity.     

Returning home after Superstorm Sandy: phases in the return-entry process

Natural Hazards - Despite current advances in research related to return-entry process following disasters, the need to understand this process from the perspective of the returnees remains. This...     

River channel evolution and tsunami impacts recorded in local sedimentary archives – the ‘Fiume Morto’ at Ostia Antica (Tiber River,Italy)

Ostia, the ancient port of Rome at the mouth of the river Tiber (Italy), flourished until the 2nd Century ad , although massive siltation had already caused the abandonment of its lagoonal harbour in the 1st Century bc . In search of an alternative harbour site, geomorphological and geoarchaeological research was carried out in the ‘Fiume Morto’, an abandoned Tiber meander to the immediate north of the town. To reconstruct its complex development and evaluate the suitability of the river channel as a fluvial harbour, a transect perpendicular to the former river channel's flow axis was systematically explored by a new methodological approach combining electrical resistivity tomography, direct push-electrical conductivity sensing and vibracores. Together with microfossil, mineralogical and geochemical analyses of sediment samples and 50 radiocarbon dates, in-depth stratigraphic data support a detailed reconstruction of different Tiber channel generations. Results reveal a meander development closely related to distinct ‘cut and fill’ dynamics. Inactive river channels were repeatedly filled with lagoonal sediments, abruptly followed by phases of intense incision. Yet, the overall Fiume Morto channel structure remained stable and almost in situ over centuries, showing nearly no lateral changes since the southward shift of the Tiber River mouth in the first millennium bc . In the 1st Century bc , channel conditions favoured navigation and transportation of goods with large ships. Open-water conditions, suitable for anchoring and landing activities, dominate from the 1st Century ad until 1557 ad , when the Fiume Morto meander was finally cut off and silted up within a short time. Within the fluvial deposits, sediments most likely related to tsunami inundations are preserved. These events occurred sometime before the 2nd Century bc , in the early 1st Century ad and in the 17th Century ad or later. Results are consistent with traces of tsunami influence found in Ostia's western lagoonal harbour.     

Tracing tsunami signatures of the ad 551 and ad 1303 tsunamis at the Gulf of Kyparissia (Peloponnese,Greece) using direct push in situ sensing techniques combined with geophysical studies

The western Peloponnese was repeatedly hit by major tsunami impacts during historical times as reported by historical accounts and recorded in earthquake and tsunami catalogues. Geological signatures of past tsunami impacts have also been found in many coastal geological archives. During the past years, abundant geomorphological and sedimentary evidence of repeated Holocene tsunami landfall was found between Cape Katakolo and the city of Kyparissia. Moreover, neotectonic studies revealed strong crust uplift along regional faults with amounts of uplift between 13 m and 30 m since the mid-Holocene. This study focuses on the potential of direct push in situ sensing techniques to detect tsunami sediments along the Gulf of Kyparissia. Direct push measurements were conducted on the landward shores of the Kaiafa Lagoon and the former Mouria Lagoon from which sedimentary and microfaunal evidence for tsunami landfall are already known. Direct push methods helped to decipher in situ high-resolution stratigraphic records of allochthonous sand sheets that are used to document different kinds of sedimentological and geomorphological characteristics of high-energy inundation, such as abrupt increases in grain size, integration of muddy rip-up clasts and fining upward sequences which are representative of different tsunami inundation pulses. These investigations were completed by sediment coring as a base for local calibration of geophysical direct push parameters. Surface-based electrical resistivity tomography and seismic data with highly resolved vertical direct push datasets and sediment core data were all coupled in order to improve the quality of the geophysical models. Details of this methodological approach, new in palaeotsunami research, are presented and discussed, especially with respect to the question of how the obtained results may help to facilitate tracing tsunami signatures in the sedimentary record and deciphering geomorphological characteristics of past tsunami inundation. Using direct push techniques and based on sedimentary data, sedimentary signatures of two young tsunami impacts that hit the Kaiafa Lagoon were detected. Radiocarbon age control allowed the identification of these tsunami layers as candidates for the ad 551 and ad 1303 earthquake and tsunami events. For these events, there is reliable historical data on major damage on infrastructure in western Greece and on the Peloponnese. At the former Mouria Lagoon, corroborating tsunami traces were found; however, in this case it is difficult to decide whether these signatures were caused by the ad 551 or the ad 1303 event.     

Dimensional analysis of yielding and pounding structures for records without distinct pulses

The seismic response of two fundamental mechanical configurations of earthquake engineering, the elastic–plastic system and the pounding oscillator, is revisited with the aid of dimensional analysis. Starting from the previous work of the authors which focused on pulse-type excitations, the paper offers an alternative, yet physically motivated, way to present the response of yielding and pounding structures under excitations with arbitrary time history. It is shown, that when the appropriate time and length scales are adopted, dimensional analysis can be implemented and remarkable order emerges in the response. Regardless of the acceleration level and frequency content of the excitation, all response spectra become self-similar and when expressed in dimensionless terms, resulting from dimensional analysis, follow a single master curve. The study proposes such scales together with the associated selection criteria among the available in literature strong ground motion parameters and shows that the proposed approach reduces drastically the scatter in the response.     

A distribution law for relative humidity in the upper troposphere and lower stratosphere derived from three years of MOZAIC measurements

Data from three years of MOZAIC measurements made it possible to determine a distribution law for the relative humidity in the upper troposphere and lower stratosphere. Data amounting to 13.5% of the total were obtained in regions with ice supersaturation. Troposphere and stratosphere are distinguished by an ozone concentration of 130 ppbv as threshold. The probability of measuring a certain amount of ice supersaturation in the troposphere decreases exponentially with the degree of ice supersaturation. The probability of measuring a certain relative humidity in the stratosphere (both with respect to water and ice) decreases exponentially with the relative humidity. A stochastic model that naturally leads to the exponential distribution is provided. Mean supersaturation in the troposphere is about 15%, whereas ice nucleation requires 30% supersaturation on the average. This explains the frequency of regions in which aircraft induce persistent contrails but which are otherwise free of clouds. Ice supersaturated regions are 3-4 K colder and contain more than 50% more vapour than other regions in the upper troposphere. The stratospheric air masses sampled are dry, as expected, having mean relative humidity over water of 12% and over ice of 23%, respectively. However, 2% of the stratospheric data indicate ice supersaturation. As the MOZAIC measurements have been obtained on commercial flights mainly between Europe and North America, the data do not provide a complete global picture, but the exponential character of the distribution laws found is probably valid globally. Since water vapour is the most important greenhouse gas and since it might enhance the anthropogenic greenhouse effects via positive feedback mechanisms, it is important to represent its distribution correctly in climate models. The discovery of the distribution law of the relative humidity makes possible simple tests to show whether the hydrological cycle in climate models is represented in an adequate way or not.     

PARTICIPATORY LEARNING AND TRAINING FOR DEVELOPMENT

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Using evidence of non-linear induced polarization for detecting extended ore mineralizations

The discrimination between electrolytic and electronic conductors is highly relevant to geological modelling as it allows conclusions to be drawn about the formation and mineral composition of rocks. The induced polarization (IP) method, which compares the electric current injected into the ground with the corresponding earth potential differences can be used for this purpose.
  This paper describes a new method based on the theory that non-linear electrochemical processes on the surface of electronic conductors are responsible for non-linear IP (NLIP) phenomena. This results in multiples of the fundamental frequency being observed in the telluric voltage spectra when a monochromatic current signal is fed into the ground. The non-linearity of the current–voltage characteristic is most effectively described by a spectral method.
  A laboratory experiment was carried out, using an electrolytic trough with a small graphite cylinder serving as an electronic conductor, which clearly demonstrated the validity of the method. A field experiment was undertaken at a borehole of approximately 450  m depth, located in the transition zone of the Tepla-Barrandium and Moldanubicum in East Bavaria. A sinusoidal current was injected into the ground using a logging tool at depths varying between 150 and 450  m. The corresponding potential differences were simultaneously observed along a profile on the surface. Field and laboratory results show a striking similarity. It can be concluded that an extensive electronic conductor—probably graphite—is steeply dipping southwards meeting the borehole at approximately 310  m depth.