Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea

Heat shock proteins 70 (hsp70) are known to be induced by a great variety of chemical stressors. The effects of different environmental contaminants, which were identified in sediments of the North Sea and the Baltic Sea as main contaminants or as contaminants of special toxicological interest, on the extent of expression of hsp70 in a permanent cell line Epithelioma papulosum cyprini (EPC) were investigated. Concentrations of the single contaminants leading to elevated hsp70 levels were compared to the respective concentrations of these contaminants encountered in the sediment extracts thus allowing an assessment of their contribution to the toxicity of these sediments. Analysis of hsp70 was performed using western blotting techniques with subsequent comparative quantification by densitometry. Eleven of thirteen contaminants have shown a dose-dependent increase in hsp70 levels. An expression of hsp70 was observed in most cases between 0.1 and 10 microg/mL test solution. Some of the investigated stressors led to extremely high hsp70 contents even at low concentrations. However, in most cases contaminant concentrations in sediments were too low to cause an effect. For this reason, effects of the tested sediments are attributed to other contaminants or rather to mixtures of stressors.     

Inter-basin movements of Mediterranean sperm whales provide insight into their population structure and conservation

The sperm whale is one of the very few deep diving mammal species in the Mediterranean Sea. Following a rare mass stranding of male sperm whales in the Adriatic Sea in December 2009, photo-identification methods were used in order to investigate previous sightings of the stranded whales in the region. Fluke photos of the stranded whales were compared with those of 153 and 128 free-ranging individuals photographed in the western and eastern Mediterranean basins, respectively. Three out of the seven stranded whales had been previously photo-identified and some of them more than once. To reach the stranding place, two of these re-identified whales performed long-range inter-basin movements of about 1600-2100 km (in a straight line) either through the Strait of Sicily or the Strait of Messina. In addition, comparisons among all whales photographed in the two Mediterranean basins revealed that one more individual first photographed in the western basin (1991) was re-identified 13 years later in the eastern basin (2004). These three cases provide the first conclusive evidence of inter-basin movement of sperm whales in the Mediterranean Sea. Inter-basin gene flow is important for the survival of the small and endangered Mediterranean sperm whale population. Mitigating the disturbance created by human activities in the straits area is crucial for its conservation.     

Integration of biochemical, histochemical and toxicogenomic indices for the assessment of health status of mussels from the Tamar Estuary, U.K

The aim of this study was to examine whether a combination of biochemical, histopathological and toxicogenomic data could be used as a valuable tool for the assessment of biological risk associated with pollutants within the Tamar River and Estuary, S.W. England, U.K. Accordingly, biochemical and histopathological biomarkers (protein carbonyls, lipofuscin, neutral lipids, lysosomal stability [N-acetyl-β-hexosaminidase and neutral red], lysosomal volume, ferric reducing antioxidant power [FRAP] and malonaldehyde [MDA]) and gene expression profiles were assessed in 5 sites from the Tamar River and Estuary (Neal Point, Town Quay, Wilcove, Cremyll Ferry and Whitsand; and a reference site, Trebarwith Strand, N. Cornwall). PAHs were measured in mussel tissue and sediment and metals were measured in mussel tissue only. Data from the biomarkers was integrated into a Mussel Expert System (MES) model to produce a simple assessment of mussel stress. Clear gradients of mussel toxicity were identified by the biomarkers (with the exception of neutral lipids) with the highest impacted animals found furthest up the Tamar, whilst the MES was unable to identify a gradient of effect. Gene expression profiles also indicated a gradient of stress with the greatest number of significantly up- or down- regulated genes found at the uppermost 2 sites. The MES did, however, determine that mussels from all sites, except the reference site, were highly stressed; a conclusion that could not be inferred from the biomarker data alone. It is concluded that the MES is a valuable tool that permits integration and interpretation of complex sets of biomarker data by identifying the biological meaning of biomarker changes.     

Two-layer quasi-geostrophic model of contour dynamics for a round basin

A quasi-geostrophic contour dynamics model permitting one to study flows induced by a system of vortex patches in a two-layer ocean with round shore boundaries in the presence of specified background flows caused by the bottom relief, the β effect, and sources and sinks at the boundaries is proposed. The principal relations of the model are presented and the algorithm of its numerical realization is described. Some experimental results of the study of the evolution of unstable two-layer vortices are demonstrated.     

Primary production and fluxes of organic carbon to the seabed in the Russian Arctic seas as a response to the recent warming

The primary production and fluxes of organic matter to the seabed and their variations were estimated in the Norwegian, Greenland, Barents, Kara, Laptev, East Siberian, and Chukchi seas in 2003–2008 on the basis of satellite and field data. When counting the open water area with the assumptions made for the assessment of the primary production in the regions hidden under clouds, the reliable trends of its variability (increasing) were revealed only in the Greenland, Barents, and Kara seas.     

Composition and secondary alterations of microfossils in sediments of the Ashadze-1 hydrothermal field (tropical Mid-Atlantic Ridge)

The first thorough analysis of microfossils from ore-bearing sediments of the Ashadze-1 hydrothermal field in the Mid-Atlantic Ridge sampled during cruise 26 of the R/V Professor Logachev in 2005 revealed the substantial influence of hydrothermal processes on the preservation of planktonic calcareous organisms, as well as on the preservation and composition of the benthic foraminifers. From the lateral and vertical distribution patterns and the secondary alterations of the microfossils, it is inferred that the main phase of the hydrothermal mineralization occurred in the Holocene. Heavy metals (Cu, Co, Cr, and Ag) were accumulated by foraminiferal tests and in their enveloping Fe-Mn crusts. The distribution of authigenic minerals replacing foraminiferal tests demonstrates local zoning related to the hydrothermal activity. There are three mineral-geochemical zones defined: the sulfide zone, the zone with an elevated Mg content, and zone of Fe-Mn crusts.     

InSAR detection of residual settlement of an ocean reclamation engineering project: a case study of Hong Kong International Airport

Man-made land or islands that are reclaimed from the sea are suitable for building airports, harbors, and industry parks for material transportation because of their broad air and land spaces. However, the reclaimed foundation settlement process is of public concern, including the continuous impact of ocean processes on its stability. The majority of the buildings and facilities of Hong Kong International Airport (HKIA) are built on a reclaimed foundation. The reclaimed foundation has been in residual settlement since completion of the filling project in 1994. In this study, we use persistent scatterer interferometry (PSI) and ENVISAT (European Satellite) advanced synthetic aperture radar (ASAR) data to detect the residual settlement rates from 19 April 2006 to 9 January 2008. We use ground truth data to develop empirical correction models for correcting systematic biases in the ASAR PSI-detected settlement rates. The corrected data follow the Lorentz distribution well, implying that the residual settlement process is dominated by two modes or categories of settlement rates. The first category represents a relatively stable state and the second category represents a continuous settlement state. A ground settlement rate map of HKIA shows that an area of the Passenger Terminal Building and an area of the Southern Runway are two relatively stable areas. There are two major continuous settlement areas. One covers the airport Midfield. Another is along the coastline, implying that attention should be paid to impacts of ocean processes on the stability of airport foundations.     

A review of the equations used to predict the velocity distribution within a ship’s propeller jet

Predicting the velocity within the ship’s propeller jet is the initial step to investigate the scouring made by the propeller jet. Albertson et al. (1950) suggested the investigation of a submerged jet can be undertaken through observation of the plain water jet from an orifice. The plain water jet investigation of Albertson et al. (1950) was based on the axial momentum theory. This has been the basis of all subsequent work with propeller jets. In reality, the velocity characteristic of a ship’s propeller jet is more complicated than a plain water jet. Fuehrer and Römisch (1977), Blaauw and van de Kaa (1978), Berger et al. (1981), Verhey (1983) and Hamill (1987) have carried out investigations using physical model. Current paper reviews the state-of-art of the equations used to predict the time-averaged axial, tangential and radial components of velocity within the zone of flow establishment and the zone of established flow of a ship’s propeller jet.