Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain, genetic study on variation was conducted to its parents and offsprings of F6, F7,Fs, and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes, 297 loci ranging from 200 to 3 000 bp were obtained in the average of 9,9 loci for each primer, This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.321 2-0.476 7, and the maximum was between F7 and Fs (0.476 7). Identity analysis showed that F6 generation was more close to the female parent (0.659 3), and F7 generation was more close to the male parent (0.5788). To the sporophytes study in 24 selected primers for RAPD amplification, 191 loci ranging from 230-2 800 bp were obtained, in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.223 9),female parent (0.107 2), F6 (0.216 4), F7 (0.228 6), F8 (0.229 6) and F9 (0.317 2). The nearest genetic distance was 0.083 5 (Fs, F9). Total heterozygosity (HT) ofF6, F7, F8 and F9 generations was 0.318 6, the average heterozygosity (Hs) for F6,F7, F8 and F9 generations was 0.248 0, and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selecting Laminaria. 相似文献
河流阶地作为河谷中常见地貌, 其堆积物特征对气候变化过程研究具有重要的意义.通过对循化黄河Ⅲ级阶地剖面沉积特征、粒度、磁化率、孢粉以及光释光测年的研究, 初步厘定循化黄河Ⅲ级阶地形成时代为75 ka.循化盆地晚更新世气候演化可以大致划分为6个阶段: 120~114 ka, 气候暖湿; 114~105 ka, 气候较为干冷; 105~98 ka, 气候较暖湿; 98~85 ka, 气候转为温凉; 85~75 ka, 气候暖湿; 75~63 ka, 气候干冷.这6个阶段分别与MIS5e-4段相对应. 相似文献
A 3-D coupled ice sheet model, GLIMMER model is introduced, and an idealized ice sheet experiment under the EISMINT-1 criterion of moving boundary condition is presented. The results of the experiment reveal that for a steady-state ice sheet profile the characteristic curves describe the process of evolution which are accordant with theoretical estimates. By solving the coupled thermodynamics equations of ice sheet, one may find the characteristic curves which derived from the conservation of the mass, energy and momentum to the ice flow profile. At the same time, an agreement, approximate to the GLIMMER case and the confirmed theoretical results, is found. Present study is explorihg work to introduce and discuss the handicaps of EISMINT criterion and GLIMMER, and prospect a few directions of the GLIMMER model. 相似文献