转“全鱼”溶菌酶基因大菱鲆的研究

用大西洋条鳕(Macrozoarces americanus)抗冻蛋白启动子opAFP promoter和牙鲆(Paralichthys olivaceus)c型溶菌酶基因构建“全鱼”溶菌酶基因元件opAFP—ly，首次应用电脉冲精子介导法将该基因片段导入到大菱鲆(Scophthalmus maximus)受精卵内，获得原代转基因大菱鲆。先后采用单因子方法和正交实验方法，重复实验确定了最适导入条件：脉冲电压为400V／cm，脉冲次数为5，脉冲宽度为25ms，外源DNA浓度为50mg/L。利用最适导入条件，实现基因元件大规模转移。采用PCR技术对1月龄的原代转基因大菱鲆仔鱼的染色体DNA进行分析，结果表明外源基因的整合率可达28％。     

层结流体中的非线性惯性重力内波

根据地球流体力学基本方程组,在密度垂直层结的情况下,引进行波坐标,研究非线性定形波在相平面上的几何拓扑结构。严格论证了不存在定形孤立波,并通过Ｈａｍｉｌｔｏｎ函数及其角作用变把行波系统化成最简形式,由此而得到非线性惯性重力内波的解析解。     

The algorithms of chlorophyll-a concentration for CMODIS

The algorithms of extracting chlorophyll-a(Chl-a) concentration have been established for Chinese moderate resolution imaging spectrometer(CMODIS) mounted on Shenzhou-3 spaceship launched on 25 March 2002.The CMODIS is an ocean color sensor with 30 visible channels and 4 infrared channels,much different from other ocean color satellites and needs new algorithms to process data.Three models of Chl-a concentration were established based on Chl-a data retrieved from sea-viewing wide field-of-view sensor(SeaWiFS),with the average relative errors of 26.6%,24%.0% and 33.5%,respectively.This practical and economic approach can be used for developing the algorithms of Chinese ocean color and temperature sensor(COCTS) on the satellite Haiyang-1 to derive the Chl-a concentration concentration distribution.The applicability of the algorithms was analyzed using some in situ measurements.Suspended sediment is the main factor influencing the accuracy of the spectral ratio algorithms of Chl-a concentration.The algorithms are suitable to using in the regions where suspended sediment concentrations(SSC) are less than 5 g/m3 under the condition of relative error of Chl-a concentration retrieval within 35%.High concentration of suspended sediment leads to the overestimate remote sensing retrieval of concentration of Chl-a,while low-middle SSCs lead to the low Chl-a concentration values using the spectral ratio algorithms.Since the accuracy of Chl-a concentration by the spectral ratio algorithms is limited to waters of Case 2,it is necessary to develop semi-analytical models to improve the performance of satellite ocean color remote sensing in turbid coastal waters.     

AEL—1型风向,风速仪

本文介绍并叙述了AEL-1型风向、风速仪的设计原理,仪器各部分的电路设计,工作过程及系统软件。该仪器适用于气象台站、工矿企业、海上浮标及科研单位等部门。     

Delaunay三角网增点生长构造法研究

论述了一种由计算机自动生成Delaunay三角网的增点生长构造法。该方法属于不规则三角网的动态构网法，它借鉴了静态构网法中递归生长算法的部分思想，并利用Lawson提出的最大最小(MAX—MIN)角度法则对三角网进行即时的局部优化，从而达到了快速、有效地构建Delaunay三角网的目的。该方法对快速生成海底DEM数据，进行海底仿真具有重要的作用。     

海洋作业渔船管理系统的设计与运行试验

对渔政管理指挥系统建设项日中的业务软件部分渔船管理系统的设计方案进行了介绍。根据我国渔洋作业渔船的实际管理模式，提出了渔船管理系统设计方案。系统通过网络化的业务处理和管理流程，规范渔船建造、审批、登记以及捕捞许可证发放等管理业务，实现渔船控制指标的统一分配，以控制捕捞业发展规模和保护我国的渔业资源。系统以Microsoft VB6．0，Delphi6．0开发，在Window98／2000／XP环境下运行。通过运行试验，对系统运行环境进行了评估，为渔政管理信息化的建设、系统升级提供了经验和技术依据。     

Nonlinear Static Finite Element Stress Analysis of Pipe-in-Pipe Risers

Owing to the complexity of the pipe-in-pipe (PIP) riser system in structure, load and restraint, many problems arise in the structural analysis of the system. This paper presents a new method for nonlinear static finite element stress analysis of the PIP riser system. The finite element (FE) model of the PIP riser system is built via software AutoPIPE 6.1. According to the specialties of a variety of components in the PIP riser system, different elements are used so as to model the system accurately. Allowing for the complication in modeling the effects of seabed restraint, a technique based on the bilinear spring concept is developed to calculate the soil properties. Then, based on a pipeline project, the entire procedure of stress analysis is discussed in detail, including creation of an FE model, processing of input data and analysis of results. A wide range of loading schemes is investigated to ascertain that the stresses remain within the acceptable range of the pipe material strength. Finally, the effects of the location of flanges, the thermal expansion of submarine pipelines and the seabed restraint on stress distribution in the riser and expansion loop are studied, which are valuable for pipeline designers.     

往复流中双向导向叶冲动透平模型性能试验研究

研制了 30 0双向自调导叶透平试验机组 ,安装在 10 0 0活塞式往复流透平试验台上 ,对两种叶轮方案和 6种不同喷咀、扩压器进行了不同活塞行程、不同周期和不同恒定输出电压下性能试验。试验结果表明在喷咀出口角α1=2 1 5°、扩压器入口角α2 =6 5 5°和α1=2 4°、α2 =6 3°组合最佳 ,最高效率达 5 3 3% ,而且在宽广流量系数范围内 ,特别是大流量系数范围内都有较高效率。大大优于常用对称翼透平。将上下游导叶固定 ,变成双向固定导叶透平。用No 1叶轮和 6种导叶出口角组合进行试验。表明最佳导叶出口角为 2 4°～ 30°,最高效率仍达 38 5 % ,比对称翼透平仍高许多     

南海北部海区上层水体平均声速场的变化

对所收集到的南海北部海区的温度、盐度和深度历史资料进行了声速的计算、统计与分析,结果表明该海区上层水体的平均声速场有较明显的年际变化:表层声速的平面分布显示出沿岸水与外海水强弱交替变化的特征,声速等值线的走向几乎与岸线平行,等声速线的值自近岸向外海增加,大陆架外缘海区声速的水平梯度较大;下层声速的分布以环流和水体共同作用的形式出现,50m层声速平面分布的趋势除冬季与表层稍为相似外,其余季节与表层有明显的差别,春、夏季节50m层声速自西向东增加,而秋季与其表层分布相反,自近岸向外海减小;声速的垂直分布受海水升温与降温的影响显著,春、夏季表层海水升温,海表声速最大,声速自海表随深度的增加而减小;秋、冬季表层附近水层降温,声速稍偏低,普遍出现正梯度现象,最大声速移至表层以下的水层,这个深度随季节的改变而改变,随海区的不同而不同.该海区的平均声速有年波动现象,表层波幅最大,随深度的增加波幅变小,至100m水层其波动的位相几乎与表层相反.     

Chemistry of marine resources-ⅩⅨ. The molecule design and synthesis of crow-link type alkyd resin and its enrichment behaviour for potassium ion in sea water

This paper first presents the molecule design and synthesis of crown-link type alkyd resin,and its enrichment behaviour for potassium ion in low concentration solution and sea water.On the basis of some theory concepts of Host-Guest chemistry developed in recent years,we have designed and synthesized a new polymer enrichment agent of crow-link resin for potassiumion,i.e.,polymacrocyclic ether bislactone of diethylene glycolpyromyllitic acid(briefly called crown-link type alkyd resin or PBL resin).The yield was about 97.4%,almost quantitatively.