甲壳动物线粒体DNA的研究

对于甲壳动物的分子生物学研究特别是其中与进化相关的研究工作目前主要围绕着线粒体基因序列开展。在近20年的生物线粒体序列研究过程中线粒体全序列的数量经历了一个飞速发展的过程，但甲壳动物中仅有8个物种的线粒体全序列完成测序。通过对NCBI(National Center for Biotechnology Infomation)的GenBank中数据的分析我们发现线粒体基因序列的数量占全部序列数的近一半。甲壳动物线粒体序列的研究主要围绕着12s rRNA、16s rRNA和COI进行，国内的相关研究处于起步阶段。     

Fluxes and sources of suspended organic matter in an estuarine turbidity maximum region during low discharge conditions

Water column concentrations of total suspended solids (TSS), particulate organic carbon (POC) and particulate nitrogen (PN) were measured at three different depths in four different locations bracketing the estuarine turbidity maximum (ETM) along the main channel of a temperate riverine estuary (Winyah Bay, South Carolina, USA). Measurements were carried out over full tidal cycle (over 24 h). Salinity, temperature, current magnitude and direction were also monitored at the same time throughout the water column. Tidally averaged net fluxes of salt, TSS, POC and PN were calculated by combining the current measurements with the concentration data. Under the extreme low river discharge conditions that characterized the study period, net landward fluxes of salt were measured in the lower part of the study area, suggesting that the landward transport through the main channel of the estuary was probably balanced by export out through the sides. In contrast, the net fluxes of salt in the upper reaches of the study area were near zero, indicating a closed salt balance in this part of the estuary. In contrast to salt, the net fluxes of TSS, POC and PN in the deeper parts of the water column were consistently landward at all four sites in Winyah Bay indicating the non-conservative behavior of particulate components and their active transport up the estuary in the region around the ETM.The carbon contents (%POC), carbon:nitrogen ratios (org[C:N]a) and stable carbon isotopic compositions (δ¹³C_POC) of the suspended particles varied significantly with depth, location and tidal stage. Tidally averaged compositions showed a significant increase up the estuary in the %POC and org[C:N]a values of suspended particles consistent with the preferential landward transport of carbon-rich particles with higher vascular plant debris content. The combination of tidal resuspension and flood-dominated flow appeared to be responsible for the hydrodynamic sorting of particles along the estuary that resulted in denser, organic-poor particles being transported landward less efficiently. The elemental and isotopic compositions indicated that vascular C₃ plants and estuarine algae were the major sources of the particulate organic matter of all the samples, without any significant contributions from salt marsh C₄ vegetation (Spartina alterniflora) and/or marine phytoplankton.     

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

太湖表面定振波的数值计算和最大熵谱分析

利用水动力学方程对太湖表面的定振波进行计算，算得定振波周期约为４５２ｍｉｎ，另外，利用１９９２年８月２９－３１日在太湖西山观测到的水位资料，采用最大熵谱法，分析太湖表面的定振波，得周期值约为４５０ｍｉｎ。计算和分析的周期值基本吻合，取熵谱分析结果得太湖表面的单节点定振波周期值为４５０ｍｉｎ。     

Nonlinear tidal waves in a kind of estuary with gradually varying cross-section

-Nonlinear tidal waves in a kind of estuary are studied in the paper using one-dimensional nonlinear hydrody-namic equations with friction. The estuary has exponentially varying width B=B0 e-bx and uniform depth h. The one-dimensional hydrodynamic equations are solved by perturbation method. It was found that our solution included two special cases, Pelisenpeki's solution and Airy's solution. The former can be got by letting b=0 in our solutions, and the latter by setting 6 = 0 and f= 0 (f is linear frictional coefficient). In terms of the second-order solution, the physical mechanism of nonlinear tidal waves in estuaries with gradually varying cross-section is explored. It is shown that, under the assumption of linear friction coefficient, shallow water constituent waves consist of two parts, one is produced by shallow water nonlinear effect outside the estuary, the other is generated by shallow water nonlinear effect inside estuary. In addition, the physical mechanism of the residual tidal current and     

浅海桶形基础平台抗拔力与抗倾稳定分析

平台在海上受风、浪、流、冰等水平载荷作用产生倾斜 ,而土壤粘接力、摩擦力和土抗力是平台的抗拔和抗倾力 ,桶基平台应满足抗拔、抗倾稳定的要求 ,以保证平台的整体稳定性。讨论了桶基平台受水平力作用时 ,桶形基础的破坏模式及其计算方法 ,阐述了抗倾稳定计算方法     

Numerical Study on Instantaneous Discharge of Unsorted Particle Cloud in Cross Flow

The mixing characteristics of particles such as dredged sediment of variable size discharged into cross flow are studied by a 3D numerical model, which is developed to model the particle-fluid two-phase flow. The Eulerian method with the modified k-ε parameterization of turbulence for the fluid phase is used to solve fluid phase, while a Lagrangian method for the solid phase (particles), both the processes are coupled through the momentum sources. In the model the wake turbulence induced by particles has been included as additional source term in the k-ε model; and the variable drift velocities of the particles are treated efficiently by the Lagrangian method in which the particles are tracked explicitly and the diffusion process is approximated by a random walk model. The hydrodynamic behavior of dumping a cloud of particles is governed by the total buoyancy of the cloud, the drag force on each particle and the velocity of cross-flow. The computed results show a roughly linear relationship between the displacement of the frontal position and the longitudinal width of the particle cloud. The particle size in the cloud and the velocity of cross flow dominate the flow behavior. The computed results are compared with the results of laboratory experiments and satisfactory agreement is obtained.     

One new species of free-living marine nematodes (Enoplida,Anticomidae, Cephalanticoma) from the Huanghai Sea

One new species of free-living marine nematodes Cephalanticoma filicaudata sp. nov. from the Huanghai Sea, China is described and illustrated. Cephalanticoma filicaudata sp. nov. is characterized by well developed cephalic capsule, three teeth at the anterior of oesophagus, excretory pore opens posterior to cervical setae, only two cervical setae per file, body length longer than 5 200 μm, tail long and no ventral-lateral seta between the supplement and the cloacal opening. Types are deposited in the College of Marine Life Sciences, Ocean University of China.     

大黄鱼病原弧菌拮抗放线菌的筛选与人工诱变

从厦门集美海滩底泥分离得到38株放线菌，用琼脂块法测定它们对大黄鱼病原弧菌-溶藻弧菌和副溶血弧菌的拮抗效果，选择两株拮抗效果较好的放线菌进行诱变，测定诱变后各菌株的抗菌效果，并选择抗菌效果较好的菌株进行第二次诱变，如此反复诱变3次，共得到97株放线菌，结果表明；38株放线菌中的22株对两株病原菌有一定的拮抗作用；用紫外线照射可以获得少量对两株病原菌拮抗作用加强的菌株。     

不同软基处理方法适用性比较

总结分析了国内外现有的软土地基处理的各种方法。通过对采用不同方法进行软基处理的工程实例处理效果的定量对比，发现不同软基处理方法的适用性差异很大，多数工程中仅用一种方法很难达到设计要求，而两种或多种方法联合则可望取得更好的效果。