凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。     

清淤输泥的新方法新设备

为解决中低滩涂沉积的深厚软粘土层的输送难题,提出了一种清淤输泥的新方法,研制了能远距离输送高浓度、大粘度、低含水量的淤泥和软粘土的土方施工新设备:包括进料口、推送器、表面膜发生器、脉冲切割器、定位系统、智能控制系统、输料管道、管道浮子等。该设备具有能耗低,工效高,效益好;结构简单,故障少,运行维护方便;定位准确性高;自动化程度高等特点。可广泛应用于水利、环保、建筑工程中的淤泥和软粘土输送。     

浙江省海塘塘顶高程的确定

介绍了浙江省海塘塘顶高程确定时所涉及的波浪爬高，越浪量等的计量，同时分析了1997年后浙江省海塘塘顶高程确定的方法，由已建海塘的塘顶高程分析可见，在背水坡和塘顶有保护措施的情况下，采用允许最大越波流量来控制海塘塘顶高程，可适当降低塘身高度。     

The importance of extratropical and tropical cyclones on the short-term evolution of barrier islands along the northern Gulf of Mexico, USA

Data are presented indicating the complexity and highly variable response of beaches to cold front passages along the northern Gulf of Mexico, in addition to the impacts of tropical cyclones and winter storms. Within the past decade, an increase in the frequency of tropical storms and hurricanes impacting the northern Gulf has dramatically altered the long-term equilibrium of a large portion of this coast. A time series of net sediment flux for subaerial and nearshore environments has been established for a section of this coast in Florida, and to a lesser extent, Mississippi. The data incorporate the morphological signature of six tropical storms/hurricanes and more than 200 frontal passages.

Data indicate that (1) barrier islands can conserve mass during catastrophic hurricanes (e.g., Hurricane Opal, a strong category 4 hurricane near landfall); (2) less severe hurricanes and tropical storms can promote rapid dune aggradation and can contribute sediment to the entire barrier system; (3) cold fronts play a critical role in the poststorm adjustment of the barrier by deflating the subaerial portion of the overwash terrace and eroding its marginal lobe along the bayside beach through locally generated, high frequency, steep waves; and (4) barrier systems along the northern Gulf do not necessarily enter an immediate poststorm recovery phase, although nested in sediment-rich nearshore environments. While high wave energy conditions associated with cold fronts play an integral role in the evolution and maintenance of barriers along the northern Gulf, these events are more effective in reworking sediment after the occurrence of extreme events such as hurricanes. This relationship is even more apparent during the clustering of tropical cyclones.

It is anticipated that these findings will have important implications for the longer term evolution of barrier systems in midlatitude, microtidal settings where the clustering of storms is apparent, and winter storms are significant in intensity and frequency along the coast.     

Preliminary Study on Oil Oxidizing Degradation of Hydrocarbon

The present note describes the microbial degradation of crude oil and hydrocarbons, and discusses the microbial purification of the sea. The study shows that, (1) The ability, of Gram-negative bacteria to degrade crude oil is higher than Gram-positive bacteria. Degradation by Flavobacterium spp. and Xanthomonas spp. is higher than others; (2) The ability of dif-     

用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分     

南沙群岛海域水体漫衰减系数Kd(490)的特性研究

对南沙群岛海区水体Kd(490）的特性进行了深入分析和探讨,研究结果表明：Kd（490）的垂直分布与同步测量的海水叶绿素a浓度的垂直分布有很强的相关性;Kd（490）的垂直分布反映出该区域海水存在较强的分层现象,研究中还发现上行辐亮度漫衰减系数KL（490）与Kd（490）垂直变化趋势相当一致,但是以二者达到最大值的深度为界,上层水体的KL（490）值稍大于Kd(490）值,在这一深度以下则相反。     

海洋旅游研究初探

海洋旅游在我国发展迅猛 ,但对海洋旅游的研究明显滞后。就海洋旅游的基本概念、研究范围、特点、开发类型及开发原则等基础理论问题提出了自己粗浅的认识 ,以求引起更多的学者关注这一领域的研究 ,进行更加有益的探讨     

金属离子对锯缘青蟹NAGase活力的影响

本文研究了几种金属离子对锯缘青蟹（Scylla serrata）N-乙酰-β-D-氨基葡萄糖苷酶（NAGase）活力的影响．其结果表明：Li^＋、Na^＋和K^＋对酶活力没有明显影响，Mg^2＋、Ca^2＋和Ba^2＋对该酶均有激活作用，激活程度依次为Ca^2＋〉Ba^2＋〉Mg^2＋．Al^3＋、Fe^3＋、Cd^2＋、Pb^2＋和Hg^2＋对该酶具有一定的抑制作用，2．0μmol／dm^3的Hg^2＋可以使酶活力完全丧失．Co^2＋对酶的效应是先激活后抑制，Mn^2＋对酶仅有轻微的激活作用．Cd^2＋和Fe^2＋对锯缘青蟹NAGase的抑制作用都呈竞争性-反竞争性混合Ⅱ型效应，Cd^2＋的抑制常数Ki和KiS分别为23．9、5．0mmool／dm^3，Fe^3＋的抑制常数Ki和KiS分剐为395．5、135．6μmol／dm^3．Ki〉KIS说明酶一底物络合物（ES）与抑制剂的亲和力比游离酶（E）与抑制剂的亲和力大．     

用陆地卫星数据描绘渤海湾海河口区不同污染等级

Okamoto (1969)指出，多变量的主成分具有变差最优性、信息损失最小性、相关最忧性和回归最优性等最优性质，因此多变量的主成分分析被广泛应用于自然科学和社会科学多种领域。由于主成分可以看作所研究的某个复杂事物的一个概括(肯德尔，1975),并可根据主成分所提供的数据，按数值大小排列顺序分级，因此主成分分析也被遥感界应用于区分不同水质(Klemas et al, 1981)。 本文以渤海湾西北部海河、永定新河河口区的陆地卫星数据作主成分分析，以显示河口区海水不同污染等级。同时以河口区各站位实测的多项有机污染数据作主成分变换，按主成分数值大小，将各站位分为不同污染等级。计算表明，遥感数据显示的不同污染等级的地理分布与实测数据是一致的。