Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

石笋记录的西南地区MIS4阶段夏季风的演化

基于重庆市丰都县羊子洞石笋Y-02的12个²³⁰Th年龄和503个氧同位素数据重建了中国西南地区60~73ka B.P.时段夏季风的演化历史。Y-02的δ¹⁸O记录显示中国季风区D/O18、D/O19a和D/O19共3个千年尺度夏季风增强事件与格陵兰间冰阶（GIS）18~19相对应。此外,Y-02记录显示：紧接着D/O18事件以后,夏季风强度突然减弱,而此时北半球高纬度地区正经历着明显的降温过程。Y-02记录捕捉到的这次东亚夏季风突然且短暂的一次减弱过程,可能是H6事件或者是H6事件季风最弱的时段。Y-02的δ¹⁸O记录显示,D/O18事件是一次较强烈的千年尺度季风增强事件,持续时间近千年,而D/O19事件在持续时间和强度上不如D/O18事件,这不同于格陵兰冰芯记录。中国季风区气候变化与格陵兰地区存在着如此紧密的联系进一步支持：东亚夏季风演化受到北半球高纬度地区气候变化的影响,但是对具体的气候事件响应可能存在不同的模式。

     

铜陵新桥层状菱铁矿成因的矿物学证据及成矿意义

安徽铜陵新桥矿区二叠系栖霞组底部和石炭系黄龙组—船山组之间产出层状、似层状菱铁矿矿层。开展菱铁矿矿层成因研究对于深入剖析区域层控矽卡岩型铜铁矿床成矿机制具有重要意义。本文利用粉晶X射线衍射（XRD）、扫描电镜（SEM）对菱铁矿矿石进行矿物学研究,结果发现菱铁矿矿石主要由菱铁矿、石英、伊利石和有机质等组成,菱铁矿颗粒粒径较小,表面具有成岩自生的自形石英硬模的微结构,SEM原位微区成分分析显示菱铁矿中除了主量元素铁,还含有大量的锰、锌和钙。矿石中存在两种微结构和不同成因的石英：表面具菱铁矿硬模和次生加大结构的碎屑石英;具六方双锥、单锥以及生物成因球形的自生石英。菱铁矿矿石的组成和矿物表面微结构表明其为沉积成因,非岩浆热液起源。富有机质和亚铁的沉积菱铁矿层和沉积胶状黄铁矿层协同作用,可能是铜陵地区乃至长江中下游成矿带层状铜铁矿床层控性重要制约因素,以及可能作为燕山期中酸岩浆演化的氧化性含铜成矿流体卸载成矿的地球化学还原障。

     

南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测

研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。     

探讨建立基于Web的中国南极环境评估系统

本文根据“马德里协议”与ATCM通过的其他相关议案对南极环境管理、监测和环境影响评估的要求,在系统研究澳大利亚南极局自2003年起运行的南极环境评估系统[1]的基础上,探讨如何建立中国南极环境评估系统(Ch inese Antarctic Environm entEvaluation System,CAEES)。该系统在设计中将结合中国南极考察活动的特点、规模、发展战略以及环境保护要求,涵盖一系列静态或动态的南极环境要素数据,同时综合相关学科专家的评估意见。该系统拟采取基于W eb的数据库技术来实现南极环境的实时动态评估以及中国南极环境状况报告(Ch inese Ant-arctic Environm ental Report,CAER)的自动生成。     

Assessing seawater quality with a variable fuzzy recognition model

With the rapid development of the marine economy industry, human exploitation of marine resources is increasing, which is contributing to the growing trend of eutrophication and frequent occurrence of red tide. Accordingly, investigations of seawater quality have attracted a great deal of attention. This study was conducted to construct a seawater environmental quality assessment model based on the variable fuzzy recognition model. The uncertainty and ambiguity of the seawater quality assessment were then considered, combining the monitoring values of evaluation indicators with the standard values of seawater quality. Laizhou Bay was subsequently selected for a case study. In this study, the correct variable model for different parameters was obtained according to the linear and nonlinear features of evaluation objects. Application of the variable fuzzy recognition model for Laizhou Bay, water quality evaluation and comparison with performance obtained using other approaches revealed that the generated model is more reliable than traditional methods, can more reasonably determine the water quality of various samples, and is more suitable for evaluation of a multi-index, multi-level, nonlinear marine environment system; accordingly, the generated model will be an effective tool for seawater quality evaluation.     

黄河中游岩土侵蚀问题及防治对策探讨

黄河中游出露地层岩性主要有古生界奥陶系碳酸盐岩、石炭系和二叠系碎屑岩;中生界三叠系砂泥岩、侏罗系砂泥岩和白垩系砂泥岩;新生界古近系砂泥岩、新近系(原为第三系)红土及第四系风积黄土和现代风积沙。第四系黄土厚度大,结构松散,遇水易解体,具湿陷性,是黄河中游粗泥砂的主要物源;第四系风积沙分布于黄河中游北部,亦是黄河中游粗泥砂的主要物源。岩土侵蚀类型有五:一是风力侵蚀—风沙侵蚀;二是降雨对谷坡面的面状冲刷—坡面侵蚀;三是地表径流对谷底的侧蚀—沟谷侵蚀;四是滑塌对谷坡坡体的破坏—重力侵蚀;五是潜蚀。并对不同侵蚀类型提出了不同的防治对策。     

内蒙古黄旗海不同粒级湖泊沉积物Rb、Sr组成与环境变化

Based on the geochemical elements Rb and Sr in sediments with three different grain size fractions from profile H3 on the northern lacustrine bottomland 13 m above the Huangqihai Lake surface in 1986,the paper investigates the record of palaeolake stand state, sedimentary environmental evolution,and winter monsoon change.First,these samples are separated into three different grain size fractions,i.e.,total sediments,77-20μm and〈20μm. Second,the chemical elements-Rb and Sr-of the grain size separation were tested and analyzed systematically in this paper.Then the elements compositions of these samples are measured using VP-320 mode fluorescence spectrum instrument,respectively.The magnetic susceptibility of these samples is measured using Kappabridge KLY-3 mode instrument made in Czech AGICO Company.The results showed the elements and the ratios varied regularly with the grain size.But the ratio of Rb/Sr in the sediments〈20μm correlates positively with the magnetic susceptibility of these samples.Therefore,the ratio of Rb/Sr in the fraction〈20 μm from the lake sediments reflected the strengthening of the weathering in the deposition sites.It is a good indicator of the summer monsoon-induced weathering and pedogenesis fluctuations and can be used to reconstruct the conditions of the paleoclimate and paleoenvironment.     

273.15 到 308.15 K下二元体系（LiCl-H2O 和 MgCl2-H2O）和三元体系（LiCl-MgCl2-H2O）的体积性质研究

用高精度振动管在 273.15 至 308.15 K 的温度范围内以 5 K 的间隔对两个二元系统（LiCl-H2O 和 MgCl2-H2O）和三元系统（LiCl-MgCl2-H2O）的密度进行了实验测定密度计。基于 Vogel-Fulcher-Tamman (VFT) 方程,对 LiCl(aq) 和 MgCl2(aq) 密度与温度和摩尔浓度的相关方程的系数进行了参数化。 Young的理想混合规则被成功地应用于基于相关的二元解性质关联三元系统的密度。根据二元和三元体系的体积特性数据,根据 Pitzer 离子相互作用理论,得到 Pitzer 单盐参数和混合离子相互作用参数。在本工作中确定了三元体系在恒定离子强度下的混合体积 (ΔVm)。     

Loss of preferred habitat and pollen limitation threatens reproduction in a rare mountain <Emphasis Type="Italic">Paeonia delavayi</Emphasis>

Paeonia delavayi is a wild tree peony species endemic to high-altitude regions in southwestern China. Recent agricultural land expansion, however, not only causes its dwindling population size, but also poses a severe threat to its long-term persistence. Since our knowledge of the reproductive biology of P. delavayi is very limited, and some management misconceptions have further exacerbated the already worrisome situation, the aim of the present study is to provide some scientific evidence regarding habitat preference and breeding system of P. delavayi, and to help correct some management misconceptions. Specifically, morphological traits of P. delavayi populations growing in two different habitats (i.e., the open area versus forest understory), including basal stem diameter, plant height and flower numbers per plant, were measured and compared with each other. A range of floral manipulation experiments was further conducted to study the extent of self-compatibility and pollen limitation. The results showed that P. delavayi preferred the open habitat over forest understory as it generally grew bigger with thicker stems, and produced a higher number of flowers in the former habitat. Therefore, the open habitat should receive immediate priority for conservation action instead of being converted to agricultural land; also, P. delavayi is pollen limited, as evidenced by the fact that experimental flowers receiving supplemental non-self pollen generally displayed elevated seed production than flowers subjected to other treatments (e.g., non-supplemental self pollen, non-supplemental non-self pollen and supplemental self pollen), which is consistent with reported observations that alpine plants are more likely to be pollen-limited than lowland plants. We suggest that human intervention might be necessary to guarantee the long-term persistence of P. delavayi as harsh alpine environment, intense competition for pollinators and different anthropogenic perturbations co-limit its reproductive success.