Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Experiments on Surface Waves Interacting with Flexible Aquatic Vegetation

Surface wave interaction with aquatic vegetation appears to play a key role in coastal hydro-morpho-dynamics. As an example, the presence of a dense meadow at intermediate water depth is usually associated with a stable and resilient shore. Wave-meadow interactions are investigated here by means of physical modelling, with a focus on wave height distribution and hydrodynamics. The central part of a wave flume is covered by flexible artificial seagrass, composed of polyethylene leaves. This vegetation is tested in both near emergent and submerged conditions. The wave height reduction is evaluated by means of a drag coefficient defined from linear wave theory, which contains all the unknowns of the adopted methodology. The behaviour of such a coefficient is investigated as a function of a wave related Reynolds number. The influence of the flexibility of the leaves is also considered, together with a wave frequency parameter. The results show a complex behaviour with three different trends for near rigid, intermediate or highly flexible leaves. Amplitudes of the orbital velocities are investigated and show a fairly good match with the linear wave theory. On the contrary, the mean velocity along the water column appears to be modified by the seagrass for submerged leaves.     

Magnetic Disconnections at the Boundary of a Small Interplanetary Magnetic Flux Rope Associated with a Reconnection Exhaust

A local current sheet and a subsequent small interplanetary magnetic-flux rope were observed on 1 April 2003 by Wind and the Advanced Composition Explorer (ACE). A Petschek reconnection-like exhaust crossing of the local current sheet was identified using the Walén test. The Wind spacecraft re-entered the reconnection exhaust after the main exhaust encounter, and the reentry may be due to a spatial fold of the current-sheet surface itself. The absence of parallel strahls and the presence of antiparallel strahls on either side of the current sheet suggest that the magnetic-field lines before the exhaust and in the subsequent small flux rope are all open. The \(180^{\circ}\) pitch-angle strahls were clearly absent, and halo-suprathermal electron pitch-angle distributions were observed in the exhaust. This finding means that the open field lines of the magnetic-flux rope were reconnecting to the adjacent open field lines to produce U-shaped field lines disconnected from the Sun. These observations provide direct evidence that the magnetic fields of the interplanetary small magnetic-flux rope were disconnecting from the Sun through magnetic reconnection. This type of disconnected event potentially has important implications for the magnetic-flux budget of the heliosphere.     

Temperature evolution in the presence of anisotropic stresses

In a recent paper by Das et al. (Astrophys. Space Sci. 361:99, 2016) it was shown that the sign of the anisotropy parameter plays a pivotal role in determining the time of formation of the horizon of a collapsing radiating star. Spurred on by this observation we investigate the impact of the (an)isotropy featured in the (Das et al. in Astrophys. Space Sci. 361:99, 2016) collapsing model on the temperature profiles of the evolving system. We show that the temperature within the stellar interior is increased as the anisotropy in the pressure grows. Relaxational effects due to heat dissipation within the core further enhances the temperature at each interior point of the stellar distribution.     

Comets,Enceladus and panspermia

A growing body of evidence suggests the operation of life and life processes in comets as well in larger icy bodies in the solar system including Enceladus. Attempts to interpret such data without invoking active biology are beginning to look weak and flawed. The emerging new paradigm is that life is a cosmic phenomenon as proposed by Hoyle and Wickramasinghe (Lifecloud: the Origin of Life in the Galaxy, 1978) and first supported by astronomical spectroscopy (Wickramasinghe and Allen, Nature 287:518, 1980; Allen and Wickramasinghe, Nature 294:239, 1981; Wickramasinghe and Allen, Nature 323:44, 1986). Comets are the transporters and amplifiers of microbial life throughout the Universe and are also, according to this point of view, the carriers of viruses that contribute to the continued evolution of life. Comets brought life to Earth 4.2 billion years ago and they continue to do so. Space extrapolations of comets, Enceladus and possibly Pluto supports this point of view. Impacts of asteroids and comets on the Earth as well as on other planetary bodies leads to the ejection of life-bearing dust and rocks and a mixing of microbiota on a planetary scale and on an even wider galactic scale. It appears inevitable that the entire galaxy will be a single connected biosphere.     

Reconstruction of Horizontal Plasma Motions at the Photosphere from Intensitygrams: A Comparison Between DeepVel,LCT, FLCT,and CST

Direct measurements of plasma motions in the photosphere are limited to the line-of-sight component of the velocity. Several algorithms have therefore been developed to reconstruct the transverse components from observed continuum images or magnetograms. We compare the space and time averages of horizontal velocity fields in the photosphere inferred from pairs of consecutive intensitygrams by the LCT, FLCT, and CST methods and the DeepVel neural network in order to identify the method that is best suited for generating synthetic observations to be used for data assimilation. The Stein and Nordlund (Astrophys. J. Lett.753, L13, 2012) magnetoconvection simulation is used to generate synthetic SDO/HMI intensitygrams and reference flows to train DeepVel. Inferred velocity fields show that DeepVel performs best at subgranular and granular scales and is second only to FLCT at mesogranular and supergranular scales.     

Solar Microflares Observed by SphinX and RHESSI

In 2009, the Russian Complex Orbital Observations Near-Earth of Activity of the Sun (CORONAS-Photon) spacecraft was launched, carrying the Polish Solar PHotometer In X-rays (SphinX). The SphinX was most sensitive in the spectral range 1.2?–?15 keV, thus an excellent opportunity appeared for comparison with the low-energy end of Ramaty High Energy Solar Spectroscopic Imager (RHESSI) spectra. Common spectral measurements with these instruments cover the range where most of the flare energy is accumulated. We have chosen four consecutive small solar events observed on 4 July 2009 at 13:43 UT, 13:48 UT, 13:52 UT, and 13:55 UT (RHESSI flare peak times) and used them to compare the data and results from the two instruments. Moreover, we included Geostationary Operational Environmental Satellite (GOES) records in our analysis. In practice, the range of comparison performed for SphinX and RHESSI is limited roughly to 3?–?6 keV. RHESSI fluxes measured with a use of one, four, and nine detectors in the 3?–?4 keV energy band agree with SphinX measurements. However, we observed that SphinX spectral irradiances are three times higher than those of RHESSI in the 4?–?6 keV energy band. This effect contributes to the difference in obtained emission measures, but the derived temperatures of plasma components are similar. RHESSI spectra were fitted using a model with two thermal components. We have found that the RHESSI hot component is in agreement with GOES, and the RHESSI hotter component fits the SphinX flaring component well. Moreover, we calculated the so-called thermodynamic measure and the total thermal energy content in the four microflares that we studied. The results obtained show that SphinX is a very sensitive complementary observatory for RHESSI and GOES.     

Low resolution spectroscopy of selected Algol systems

The analysis of spectroscopic data for 30 Algol-type binaries is presented. All these systems are short period Algols having primaries with spectral types B and A. Dominant spectral lines were identified for the spectra collected and their equivalent widths were calculated. All the spectra were examined to understand presence of mass transfer, a disk or circumstellar matter and chromospheric emission. We also present first spectroscopic and period study for few Algols and conclude that high resolution spectra within and outside the primary minimum are needed for better understanding of these Algol type close binaries.     

Resonance transition periodic orbits in the circular restricted three-body problem

This work studies a special type of cislunar periodic orbits in the circular restricted three-body problem called resonance transition periodic orbits, which switch between different resonances and revolve about the secondary with multiple loops during one period. In the practical computation, families of multiple periodic orbits are identified first, and then the invariant manifolds emanating from the unstable multiple periodic orbits are taken to generate resonant homoclinic connections, which are used to determine the initial guesses for computing the desired periodic orbits by means of multiple-shooting scheme. The obtained periodic orbits have potential applications for the missions requiring long-term continuous observation of the secondary and tour missions in a multi-body environment.     

Creating an isotopically similar Earth–Moon system with correct angular momentum from a giant impact

The giant impact hypothesis is the dominant theory explaining the formation of our Moon. However, the inability to produce an isotopically similar Earth–Moon system with correct angular momentum has cast a shadow on its validity. Computer-generated impacts have been successful in producing virtual systems that possess many of the observed physical properties. However, addressing the isotopic similarities between the Earth and Moon coupled with correct angular momentum has proven to be challenging. Equilibration and evection resonance have been proposed as means of reconciling the models. In the summer of 2013, the Royal Society called a meeting solely to discuss the formation of the Moon. In this meeting, evection resonance and equilibration were both questioned as viable means of removing the deficiencies from giant impact models. The main concerns were that models were multi-staged and too complex. We present here initial impact conditions that produce an isotopically similar Earth–Moon system with correct angular momentum. This is done in a single-staged simulation. The initial parameters are straightforward and the results evolve solely from the impact. This was accomplished by colliding two roughly half-Earth-sized impactors, rotating in approximately the same plane in a high-energy, off-centered impact, where both impactors spin into the collision.