中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究

分析了几株自南海及东海分离的亚历山大藻的rDNA部分序列信息,其中包括核糖体大亚基(LSU)rDNA的5′端D1-D2区序列,以及5.8SrDNA和ITS区序列;同时也对实验室保种的部分来自其它国家和地区的亚历山大藻相关序列进行了测序和分析,并以此作为序列分析中的参考。采用ClustalX及MEGA2软件对所得到的序列信息进行了综合分析与对比。结果表明,分离自南海的塔玛亚历山大藻(Alexandriumtamarense)和分离自东海的链状亚历山大藻(A.catenella),即便是在ITS区和LSUrDNA等高变区,其序列信息也完全一致。与基因库中搜索到的其它亚历山大藻rDNA序列信息相比较,中国沿海的塔玛/链状亚历山大藻序列更接近于塔玛复合种的“亚洲温带”基因型。对于分离自南海的另外两株未定种的亚历山大藻,通过对比序列信息,发现它们与相关亚历山大藻(A.affine)非常接近。分离于我国台湾地区的微小亚历山大藻(A.minutum)在序列上与分离自新西兰的藻株相似,而与分离自欧洲的微小亚历山大藻藻株相差较大。中国沿海亚历山大藻rDNA序列信息的获得为针对有毒藻种设计特异性核酸探针,发展灵敏快速的生物检测技术奠定了基础。     

相空间反演方法在表层水温预报中的应用

利用相空间理论及方法对渤、黄、东海共4个站位近十几年的旬平均SST进行分析。结果表明：表层水温具有混饨特性，其吸引子关联维数平均约为1．23、嵌入相空间维数为6（渤、黄海）和7（东海178号站位）、二阶Renyi熵平均约为3．7×10-4（1／d）及平均可预报时间尺度平均为27个点；基于以上分析结果运用相空间反演方法建立了旬平均SST的反演模型，并且在试预报的前5旬的最大相对误差约为4．2％。     

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

不同氮源对微小亚历山大藻生长和毒素产生的影响

通过尿素、氯化铵、酵母浸出粉和硝酸钠等氮源对微小亚历山大藻(Alexandrium minutum)生长及毒素产生的影响研究,分析了微小亚历山大藻对不同氮源利用状况的差异.结果表明,在氮饥饿条件下,加入硝酸钠和酵母浸出粉能显著促进微小亚历山大藻的生长;高浓度的氯化铵在加入后对微小亚历山大藻有一定的毒性效应,表现为生长停滞,但毒性效应在5 d后消失,并得到与添加硝酸钠及酵母浸出粉相似的增长速率0.21 d-1;添加尿素对微小亚历山大藻的生长没有显著促进作用.在四种氮源中,尿素对微小亚历山大藻毒素产生的刺激作用也最弱,在稳定期每个细胞藻细胞毒素含量维持在6.00~8.00 fmol;添加硝酸钠、氯化铵和酵母浸出粉的藻细胞在稳定期毒素含量分别达到11.85,12.86和14.64 fmol.硝酸钠和氯化铵刺激藻毒素产生的效果比酵母浸出粉更为直接.四种含氮营养盐对微小亚历山大藻毒素组成的影响都很小.     

基于MODIS影像的鄱阳湖湖面积与水位关系研究

利用统计分析的方法,根据2001年获取的13景鄱阳湖区无云MODIS影像中的9景提取的水体面积,并结合同步观测的水文数据分别采用线性、对数和指数3种模型模拟湖面积-水位之间关系。结果显示对数模型相关性最好（R²=0.918）,其次为线性和指数模型。利用另外4景MODIS影像对模型进行检验表明,该模型精度较高,模拟的最大误差为3.36%。本研究显示,可根据鄱阳湖水位观测值,利用该模型预测鄱阳湖洪涝期洪水淹没面积,以弥补云天状况下光学遥感难以监测到洪水淹没范围的不足。本研究为利用遥感影像实时监控鄱阳湖水情空间动态变化提供了可行的方法,对湖泊、水库的泛洪监测、调洪功能分析具有重要意义。     

Jurassic Tectonics of North China: A Synthetic View

This paper gives a synthetic view on the Jurassic tectonics of North China, with an attempt to propose a framework for the stepwise tectonic evolution history. Jurassic sedimentation, deformation and magmatism in North China have been divided into three stages. The earliest Jurassic is marked by a period of magmatism quiescence （in 205-190 Ma） and regional uplift, which are considered to be the continuation of the “Indosinian movement” characterized by continent-continent collision between the North and South China blocks. The Early to Middle Jurassic （in 190-170 Ma） was predominated by weak lithospheric extension expressed by mantle-derived plutonism and volcanism along the Yanshan belt and alongside the Tan-Lu fault zone, normal faulting and graben formation along the Yinshan- Yanshan tectonic belt, depression and resuming of coal-bearing sedimentation in vast regions of the North China block （NCB）. The Middle to Late Jurassic stage started at 165y.5 Ma and ended up before 136 Ma; it was dominated by intensive intraplate deformation resulting from multi-directional compressions. Two major deformation events have been identified. One is marked by stratigraphic unconformity beneath the thick Upper Jurassic molasic series in the foreland zones of the western Ordos thrust-fold belt and along the Yinshan-Yanshan belt; it was predated 160 Ma. The other one is indicated by stratigraphic unconformity at the base of the Lower Cretaceous and predated 135 Ma. During this last stage, two latitudinal tectonic belts, the Yinshan-Yanshan belt in the north and the Qinling-Dabie belt in the south, and the western margin of the Ordos basin were all activated by thrusting; the NCB itself was deformed by the NE to NNE-trending structural system involving thrusting, associated folding and sinistral strike-slip faulting, which were spatially partitioned. Foliated S-type granitic plutons aged 160-150 Ma were massively emplaced in the Jiao-Liao massif east of the Tan-Lu fault zone and indicate important crustal thicken     

内蒙古桌子山煤田火区特征及灭火方法探讨

内蒙古自治区桌子山煤田赋存着较为稀缺的煤种一焦煤,煤田内存在的火区不仪烧掉了宝贵的焦性煤炭资源,给煤矿生产带来严重的事故隐患,对当地生态环境也造成极大的破坏。经查明区内有26处子火区,分布面积约760万m^2,通过煤田地质特征、煤质特征、地表指示特征、同位素氡气的浓度相对分布特征、物性特征分析了区内煤层发火原因,根据桌子山煤田火区特点,建议采用浅剥、深灌与覆盖相结合的灭火方法,并对火区进行动态监测。     

近海核电厂核素地下水释放通量的模型计算方法

为了定量计算陵区近海核电站排水管线泄漏情景下核素通过地下水途径向海洋环境的释放通量，以某近海核电站为例进行研究。首先，应用GOCAD软件建立三维地形地质模型，刻画地层的分布、剥蚀以及倾向等特点；然后，运用地下水数值模拟软件FEFLOW精细刻画丘陵区地下水系统的补给、径流和排泄特征；最后，以不被吸附滞留的核素³H和被吸附滞留的核素⁹⁰Sr、¹³⁷Cs为对象，通过实验测定了⁹⁰Sr、¹³⁷Cs在不同岩土介质中的分配系数，模拟计算了排水管线连续渗漏60 a后³H、⁹⁰Sr、¹³⁷Cs在地下水中的放射性分布及释放。结果表明：³H迁移速度基本与地下水流速一致，地下水中的最大放射性浓度为0.285 0 Bq/L，第20 000天时向收纳水域的释放通量达到最大值，约526 Bq/d；⁹⁰Sr吸附性能相对较弱，最大迁移距离约80 m，地下水中的最大放射性浓度为0.032 1 Bq/L；¹³⁷Cs吸附能力较强，相当长的时间内被滞留在管线附近，地下水中最大放射性浓度分别为6.840×10^-3 Bq/L，释放通量为0 Bq/d。由弥散度的不确定分析可知，弥散度越大，地下水中³H的最大放射性浓度越小，向海洋环境的释放通量越多。     

Flux determination over a smooth surface under strongly unstable conditions

Careful micrometeorological measurements on an empty parking lot allowed determination of the surface fluxes of sensible heatH and of momentum by applying profile equations derived from Monin-Obukhov similarity theory with two sets of the stability correction function for momentum _m and sensible heat _h . These fluxes were compared with reference values ofH independently determined by means of an eddy correlation technique. In general, better agreement was found betweenH values derived from profiles with the stability functions of Brutsaert (1992) and referenceH values, than when the Businger-Dyer functions were used to deriveH. The disagreement in the latter comparison was especially serious under strongly unstable conditions, with the value ofy=–z/L (wherez is the height andL is the Obukhov length) larger than 10. A closer look at the procedure for calculatingH from the profiles revealed that the large differences between theH values derived with these two different versions of the stability correction functions were caused by the small differences of the _h values, and not by the larger differences of the _m values. This result stems from the strong sensitivity of the resultingH values on the choice of _h .