盐酸胍对文昌鱼Branchiostoma belcheri(Gray)酸性磷酸酯酶构象与活力的影响

本文报告了文昌鱼酸性磷酸酯酶在不同浓度的盐酸胍作用下酶的构象与催化活力变化的关系。盐酸胍浓度在1mol/dm~3时,酶活力提高50%.随着盐酸胍浓度提高(2～4mol/dm~3),酶活力下降,荧光强度也下降,荧光发射光谱λ_(max)值明显红移(330～358nm)。测其变性及失活动力学常数,结果表明,酶的失活速度大于变性速度,表现为慢构象变化、快活力变化的一种模式。在盐酸胍3～4mol/dm~3时,酶的变性与失活过程表现为两个一级反应过程(快相和慢相),其构象与催化活力的变化表明AC-Pase 与活力部位相关的色氨酸残基微环境产生了变化。     

乙二醇对长毛对虾酸性磷酸酶活力与构象的影响

应用荧光光谱、紫外差示光谱及园二色性光谱等生物物理技术研究长毛对虾酸性磷酸酶经乙二醇微扰后的分子构象变化情况,结果表明乙二醇对酶分子构象有显著的影响。酶的内源荧光强度随乙二醇浓度增大而增强,Ｔｙｒ、Ｔｒｐ残基的微环境发生明显的变化;紫外差示光谱在２２０和２７５ｎｍ出现２个负峰,而２３６ｎｍ出现正峰,２２０和２３６ｎｍ的差吸收与酶分子主链去折叠有关,而２７５ｎｍ主要与Ｔｒｐ残在微环境变化有关;乙二醇     

省以下气象业务体制、预报流程建立及预报指导技术的研究

我省气象业务体系在9210工程之后,已显示出预报体制和流程不适应新的设备与技术环境的问题,影响了天气预报业务技术的发展,制约了天气在空间,时间分辨率方面的提高,因此,如何在卫星广域网高速舆网络环境下,建立一套新的预报业和作用流程以及省,地,县三级预报技术,是非常重要的。     

辽宁省岩石地球化学研究新进展

辽宁地球化学场研究,是根据辽宁全域41种元素丰度及各种地球化学参数,论述了辽宁不同地质时代和构造分区表壳岩石元素分配特点,指出了辽宁重要成矿元素的高值时代;建立了辽宁早前寒武纪变质岩、超变质岩,全域沉积岩,火成岩不同岩类的主元素地球化学模型和元素富集型式;并对Au、Pb、Zn、B等主要成矿元素的分布、分配以及与有关元素的相关特征进行了讨论;指出了成矿元素的主要载体岩石和高值地质单元。根据大地构造分区、元素组合,将辽宁全域划分为4个地球化学省,11个地球化学区,35个地球化学分区。     

长毛对虾酸性磷酸酶功能基团的研究(Ⅱ)

用化学修饰剂pCMB、NBS、K试剂修饰长毛对虾Penaeuspenicilla－tusAlcock酸性磷酸酶（ACPase，EC3．1．3．2），在修饰剂作用下，酶活力明显下降，健康虾酶对修饰剂的敏感性（NBS除外）大都稍大于病虾酶。修饰酶的紫外280um吸收光谱以及荧光340um发射光谱的变化，表明酶活力下降的同时酶构象也产生相应的变化。结果表明，酶分子的半胱氨酸残基、色氨酸残基以及门冬氨酸、谷氨酸β或γ验基与酶活力有关，是酶的催化功能基团。     

Shallow destructive earthquakes

Depths of earthquake occurrence and large slip distribution are critical for seismic hazard assessment.Numerous examples show that earthquakes with similar magnitudes,however,can result in significantly different ground shaking and damage.One of the critical factors is that whether the large slip was generated near the ground surface.In this article,we reviewed two aspects that are important on this regard,shallow slip deficit and nucleation depth.Understanding how shallow future earthquakes may nucleate in particular regions,such as shale gas fields,is critical for hazard assessment.Whether or not a strong earthquake may slip significantly at shallow depths(less than 3 km)plays crucial rules in seismic hazard preparation and should be further investigated by integrating high-resolution fault zone observations,dynamic rupture simulation,and fault zone properties.Moreover,precisely resolving shallow depth and slip distribution of earthquakes demands InSAR and/or other image data that can better capture the near-fault deformation to constrain the source parameters of earthquakes.     

Evaluating historical simulations of CMIP5 GCMs for key climatic variables in Zhejiang Province,China

Assessing the regional impact of climate change on agriculture, hydrology, and forests is vital for sustainable management. Trustworthy projections of climate change are needed to support these assessments. In this paper, 18 global climate models (GCMs) from the fifth phase of the Coupled Model Intercomparison Project (CMIP5) are evaluated for their ability to simulate regional climate change in Zhejiang Province, Southeast China. Simple graphical approaches and three indices are used to evaluate the performance of six key climatic variables during simulations from 1971 to 2000. These variables include maximum and minimum air temperature, precipitation, wind speed, solar radiation, and relative humidity. These variables are of great importance to researchers and decision makers in climate change impact studies and developing adaptation strategies. This study found that most GCMs failed to reproduce the observed spatial patterns, due to insufficient resolution. However, the seasonal variations of the six variables are simulated well by most GCMs. Maximum and minimum air temperatures are simulated well on monthly, seasonal, and yearly scales. Solar radiation is reasonably simulated on monthly, seasonal, and yearly scales. Compared to air temperature and solar radiation, it was found that precipitation, wind speed, and relative humidity can only be simulated well at seasonal and yearly scales. Wind speed was the variable with the poorest simulation results across all GCMs.     

浙江省岩石地层清理成果简介

本文介绍浙江省岩石地层单位清理成果。按照岩石地层单位划分出原则，对浙江省境域内出露的元古界—第四系进行全面清理：划分出岩石地层单位109个，其中群级11个、组级94个；在清理中新建3个组，启用6个群19个组，引用外省12个组，未采用2个群27个组。并明确各岩石地层单位的含义及时代。     

An EUV Jet and Hα Filament Eruption Associated with Flux Cancelation in a Decaying Active Region

Using data from the Transition Region and Coronal Explorer (TRACE), Solar and Heliospheric Observatory (SOHO), Ramaty High Energy Solar Spectroscopic Imager (RHESSI), and Hida Observatory (HO), we present a detailed study of an EUV jet and the associated Hα filament eruption in a major flare in the active region NOAA 10044 on 29 July 2002. In the Hα line wings, a small filament was found to erupt out from the magnetic neutral line of the active region during the flare. Two bright EUV loops were observed rising and expanding with the filament eruption, and both hot and cool EUV plasma ejections were observed to form the EUV jet. The two thermal components spatially separated from each other and lasted for about 25 minutes. In the white-light corona data, a narrow coronal mass ejection (CME) was found to respond to this EUV jet. We cannot find obvious emerging flux in the photosphere accounting for the filament eruption and the EUV jet. However, significant sunspot decay and magnetic-flux cancelation owing to collision of opposite flux before the events were noticed. Based on the hard X-ray data from RHESSI, which showed evidence of magnetic reconnection along the main magnetic neutral line, we think that all of the observed dynamical phenomena, including the EUV jet, filament eruption, flare, and CME, should have a close relation to the flux cancelation in the low atmosphere.     

河流水系连通性机制及计算方法综述

河流水系连通性具有水文调蓄、保障生物迁徙通畅、改善水土环境等功能,目前主要从水文、景观和生态等方面对其进行了定义,而河流水系连通性对社会发展也发挥着重要作用,故河流水系连通性具有自然和社会双重属性,其连通性机制主要表现为纵向、横向、垂向连通性。目前主要从景观学、水文学、水力学、生态学等学科角度提出了图论法、水文-水力学法、景观法、生物法和综合指标法等计算方法,各方法有其适用性和不足,应用中须针对实际问题选择适宜方法或进行修正。河流水系连通性未来研究应综合多尺度、多领域、多学科,在研究内容上,重点开展尺度协同、连通性含义界定、响应机理及修复问题研究,在研究手段上,重点开展多方法、多技术集成问题研究。