Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

丹东形变观测洞室改造

“十五”期间,为了新增水管仪、伸缩仪,丹东地震台对形变观测洞室进行了环境改造,新建了仪器基墩,解决了建造仪器基墩过程中遇到的施工难题,为水管仪和伸缩仪运行提供了良好的观测条件。文中介绍的方法对其他台站建造仪器基墩有一定借鉴意义。     

The influence of coupled physical swelling and chemical reactions on deformable geomaterials

Coupled thermo‐hydro‐mechanical‐chemical modelling has attracted attention in past decades due to many contemporary geotechnical engineering applications (e.g., waste disposal, carbon capture and storage). However, molecular‐scale interactions within geomaterials (e.g., swelling and dissolution/precipitation) have a significant influence on the mechanical behaviour, yet are rarely incorporated into existing Thermal‐Hydro‐Mechanical‐Chemical (THMC) frameworks. This paper presents a new coupled hydro‐mechanical‐chemical constitutive model to bridge molecular‐scale interactions with macro‐physical deformation by combining the swelling and dissolution/precipitation through an extension of the new mixture‐coupling theory. Entropy analysis of the geomaterial system provides dissipation energy, and Helmholtz free energy gives the relationship between solids and fluids. Numerical simulation is used to compare with the selected recognized models, which demonstrates that the swelling and dissolution/precipitation processes may have a significant influence on the mechanical deformation of the geomaterials.     

Three‐dimensional shakedown solutions for anisotropic cohesive‐frictional materials under moving surface loads

Previous work on three‐dimensional shakedown analysis of cohesive‐frictional materials under moving surface loads has been entirely for isotropic materials. As a result, the effects of anisotropy, both elastic and plastic, of soil and pavement materials are ignored. This paper will, for the first time, develop three‐dimensional shakedown solutions to allow for the variation of elastic and plastic material properties with direction. Melan's lower‐bound shakedown theorem is used to derive shakedown solutions. In particular, a generalised, anisotropic Mohr–Coulomb yield criterion and cross‐anisotropic elastic stress fields are utilised to develop anisotropic shakedown solutions. It is found that shakedown solutions for anisotropic materials are dominated by Young's modulus ratio for the cases of subsurface failure and by shear modulus ratio for the cases of surface failure. Plastic anisotropy is mainly controlled by material cohesion ratio, the rise of which increases the shakedown limit until a maximum value is reached. The anisotropic shakedown limit varies with frictional coefficient, and the peak value may not occur for the case of normal loading only. Copyright © 2013 John Wiley & Sons, Ltd.     

断层EDF技术在复杂断块构造研究中的应用

复杂断块构造具有断层复杂,小断层广泛发育,断层识别难度大,储层分布及连通性复杂,地震成像不清、资料品质较差等特征。对于此类问题,常规地震资料无法满足断层解释精度要求。提出了一种新的断层EDF解释性处理技术,将地震资料处理与解释融为一体,以提高资料的信噪比和断层的识别能力。断层EDF解释性处理技术是利用扩散滤波和中值倾角滤波相结合的断层增强滤波方法来提高资料的信噪比,突出断层和地层的接触关系,再在滤波后的地震资料数据体上进行分频处理,选择最佳成像频带,突出识别不同规模的断层。通过在涠洲A油田的应用,取得了良好的效果。     

P气田基于地质模型的储量不确定性分析

地质建模的最终目的是建立反映地质认识的地质模型,并对储层的不确定性以及由此带来的储量不确定性进行定量表征。南海P气田正处于开发的前期研究阶段,井点资料少,储层的纵横向变化快,储量不确定性强。该文以地质模型为载体,探讨了该气田地质储量不确定性分析的方法,总结了相似气田地质储量不确定性分析的一般方法:通过对变量的敏感性分析,确定影响该气田地质储量的主要变量,以此为基础通过拉丁超立方概率分析法进行储量评价及地质模型的优选,最终确定构造和气水界面的变化是影响该气田储量的主要因素,P50是该气田可动用储量。该方法为前期研究阶段的储量动用提供了新的思路。     

Two new species of the genus <Emphasis Type="Italic">Cobitis</Emphasis> Linnaeus (Teleostei: Cobitidae) from southern China

Two new species of the genus Cobitis from southern China, C. hereromacula from the Luohe River in Guangdong Province and C. baishagensis from the Nandujiang River in Hainan Province, are described and illustrated here. C. hereromacula can be distinguished from its congeners by possessing the following combination of characteristics: absence of the second and third pigmentary zones of Gambetta; 13–16 oval blotches on the dorsum and 10–13 vertical, elongated triangular blotches below the midlateral line with more than 20 vertical dark brown bars between them; 6–7 narrow rows of dark spots on the caudal fin; a vertical oval spot smaller than the eye diameter on the upper part of the caudal peduncle; pointed mental lobes of the lower lip pointed with a slightly filiform tip; one slender and long needle-shaped lamina circularis at the base of the first branched ray of the male pectoral fins. C. baishagensis can be distinguished from its congeners by the fourth Gambetta zone being covered by 10–12 transverse elongated blotches; 4–5 narrow rows of dark spots on the caudal fin; a vertical blotch smaller than the eye diameter on the upper part of the caudal peduncle; males with a slender and long needle-shaped lamina circularis at the second branched pectoral fin ray in males; large scales with a slightly large focal zone; undeveloped mental lobes with a lower lip that does not end posteriorly in a filiform tip.     

贵州松桃大屋锰矿床主要地质特征与找矿预测

松桃大屋锰矿是贵州省地矿局103地质队于上世纪70年代勘查评价的中型锰矿床。锰矿产于南华系大塘坡组第一段黑色岩系底部,矿体呈层状、似层状产出,矿层走向北东约70°,矿体平均厚1. 42 m,平均品位18. 85%。矿区从北西向南东,含锰岩系、锰矿体从无到有。大屋锰矿床只保留了古天然气渗漏沉积成矿系统中的过渡相和边缘相,中心相应在齐公坡一带,但已被剥蚀殆尽。通过构造古地理恢复分析,推测南华纪大屋地堑盆地长大于14 km、宽约4 km,沿北东70°方向展布。预测盆地北东柳树塘、大坳至孟溪一带还具有发现中-大型规模锰矿床的潜力。     

Estimating satellite phase fractional cycle biases based on Kalman filter

Phase fractional cycle biases (FCBs) originating from satellites and receivers destroy the integer nature of PPP carrier phase ambiguities. To achieve integer ambiguity resolution of PPP, FCBs of satellites are required. In former work, least squares methods are commonly adopted to isolate FCBs from a network of reference stations. However, it can be extremely time consuming concerning the large number of observations from hundreds of stations and thousands of epochs. In addition, iterations are required to deal with the one-cycle inconsistency among FCB measurements. We propose to estimate the FCB based on a Kalman filter. The large number of observations are handled epoch by epoch, which significantly reduces the dimension of the involved matrix and accelerates the computation. In addition, it is also suitable for real-time applications. As for the one-cycle inconsistency, a pre-elimination method is developed to avoid iterations and posterior adjustments. A globally distributed network consisting of about 200 IGS stations is selected to determine the GPS satellite FCBs. Observations recorded from DoY 52 to 61 in 2016 are processed to verify the proposed approach. The RMS of wide lane (WL) posterior residuals is 0.09 cycles while that of the narrow lane (NL) is about 0.05 cycles, which indicates a good internal accuracy. The estimated WL FCBs also have a good consistency with existing WL FCB products (e.g., CNES-GRG, WHU-SGG). The RMS of differences with respect to GRG and SGG products are 0.03 and 0.05 cycles. For satellite NL FCB estimates, 97.9% of the differences with respect to SGG products are within ±?0.1 cycles. The RMS of the difference is 0.05 cycles. These results prove the efficiency of the proposed approach.