Biotransformation of amide using Bacillus sp.: isolation strategy, strain characteristics and enzyme immobilization

Amidase hydrolases/acyltransferases are of considerable industrial interest due to potential applications in the production of useful hydroxamic acids. The test strain, capable of acetamide degradation, was isolated by an enrichment technique with acetamide as sole source of nitrogen. Based on morphology, physiological tests and biochemical tests, this isolate was identified as Bacillus sp. and on the basis of 16S Ribosomal ribonucleic acid sequence, a phylogenetic tree was drawn and was identified as Bacillus megaterium. Resting cells containing active acyltransferase enzyme were prepared and immobilized in the gel beads of sodium alginate, agar, polyacrylamide and polyvinyl alcohol?Calginate. The beads were tested for acyltransferase using Iron (III) chloride reagents at 55°C and were found to be affected by substrate concentration, type of buffer, buffer pH and reaction temperature. These factors were optimized using sodium alginate immobilized beads. This study proved useful in understanding the technique of immobilization of acyltransferase enzyme, its operational stability and its importance in the synthesis of hydroxamic acid.     

Discovery of animal eggs and embryos from the Ediacaran (Terminal Proterozoic) Chambaghat Formation,Krol Group,Himachal Lesser Himalaya

The globular to suboval microfossils with distinctively ornamented outer coverings interpreted as animal eggs and embryos have been discovered from the black phosphatic chert lentils of the Ediacaran (Terminal Proterozoic) Chambaghat Formation (Krol sandstone), Krol Group, Himachal Lesser Himalaya, India. Similar animal eggs and embryos have earlier been recorded only from the phosphorites of the uppermost Neoproterozoic Doushantuo Formation (Ca. 570±20 Ma) exposed at Weng’an, South China. Present record of eggs and embryos is comparable with extant eggs and embryos of cnidarians and bilaterians like molluscs, annelids and arthropods. The eggs and embryos from the Terminal Proterozoic rocks of India are the only one recorded from the equivalent stratigraphic horizon outside China. This discovery of eggs and embryos adds to the understanding the evolutionary trends in the Proterozoic metazoan life.     

Investigations on Influence of Bio-Geo Interface on Suction Characteristics of Fine-Grained Soil

Dependence of soil suction on factors such as formation of the soil, its particle size, mineralogy, pore-size distribution characteristics, water content, fabric, stress history and presence of salts has been extensively investigated by researchers. However, the influence of the microbial presence and its activity on the soil suction has not yet received researchers’ attention. With this in view, a methodology that facilitates investigation regarding the presence of ‘soil-microbial interface’, which has been termed as ‘bio-geo interface’, has been proposed in this study. Furthermore, the influence of this ‘interface’ on suction characteristics of such soils has also been investigated and the utility of the proposed methodology in addressing such issues, successfully, has been demonstrated.     

Hydrochemical characterization and suitability assessment of groundwater in Baga–Calangute stretch of Goa,India

The present study is the first attempt to determine the suitability of groundwater for drinking and irrigation in the Baga–Calangute stretch of Goa. The suitability of groundwater for potable use was assessed by comparing observed values against standards prescribed by the Bureau of Indian Standards, and the quality was classified based on the Weighted Arithmetic Water Quality Index. Most of the groundwater samples (90%) were found to be suitable for drinking except for hardness, chlorides, and nitrates. The percent sodium (%Na), residual sodium carbonate, soluble sodium percentage, sodium adsorption ratio, Kelly’s ratio, and Permeability Index were found to be within the prescribed limits for irrigation purposes. The major mechanism controlling groundwater chemistry, i.e., rock–water interaction, was also studied, and it was found that silicate weathering plays a major role in the dissolution of minerals. Based on the hydrochemical characterization, the water was observed to be of the Ca–Na–SO₄ composition type except for one sample which was of the Na–Cl composition type. Classification of the meteoric genesis suggested that the groundwater in surficial aquifers in the region had a deep meteoric percolation, and its chemistry is regulated by rock–water interaction.     

U–Pb geochronology,Sr–Nd isotopic compositions,geochemistry and petrogenesis of Shah Soltan Ali granitoids,Birjand, Eastern Iran

The Shah Soltan Ali area (SSA) is located in the eastern part of the Lut Block metallogenic province. In this area different types of sub-volcanic intrusions including diorite porphyry, monzonite porphyry and monzodiorite porphyry have intruded into basaltic and andesitic rocks. Zircon U–Pb dating and field observations indicate that intermediate to mafic volcanic rocks (38.9 Ma) are older than subvolcanic units (38.3 Ma). The subvolcanic intrusions show high-K calc-alkaline to shoshonitic affinity and are metaluminous. Based on mineralogy, high values of magnetic susceptibility [(634 to 3208) × 10^?5 SI], and low initial ⁸⁷Sr/⁸⁶Sr ratios, they are classified as belonging to the magnetite-series of oxidant I-type granitoids and are characterized by an enrichment in LREEs relative to HREEs, with negative Nb, Ti, Zr and Eu anomalies. These granitoids are related to volcanic arc (VAG) and were generated in an active continental margin. Low initial ⁸⁷Sr/⁸⁶Sr ratios (0.7043 to 0.7052) and positive εNd values (+1.48 to +3.82) indicate that the parental magma was derived from mantle wedge. Parental magma was probably formed by low degree of partial melting and metasomatized by slab derived fluids. Then assimilation and fractional crystallization processes (AFC) produced the SSA rocks. This magma during the ascent was contaminated with the crustal material.All data suggest that Middle-Late Eocene epoch magmatism in the SSA area, occurred during subduction of Neo-Tethys Ocean in east of Iran (between Afghan and Lut Blocks).     

Spherules from the Late Cretaceous Phosphorite of the Fatehgarh Formation, Barmer Basin, India

A first report of discovery of spherules, glassy balls, highly magnetic fine dust and microbracciated matrix in the Fatehgarh Formation of Barmer Basin, Rajasthan, India is being presented in this paper. The Fatehgarh Formation is a mixed siliciclastic, carbonate and phosphorite formation of Cretaceous age in the Barmer Basin that comprises sediments of Middle Jurassic to Lower Eocene age. The phosphorite zone in the Fatehgarh Formation is 8 metre-thick zone that comprises phosphatic sandstone, bone bed, bedded phosphorite and phosphatic and non phosphatic gastropod beds. The spherules occur in a thin phosphatic-clay mud and silt band of bone bed, which also yielded a very rich and diverse microvertebrate assemblage with a dominant Late Cretaceous (Maastrichtian) form of Igdabatis along with forms comprising of Semionodontid, Lapisosteum and Enchodontid. The end Cretaceous is marked for a mass extinction of numerous species including dinosaurs. An extraterrestrial impact is interpreted as the reason for this mass extinction. Whether these spherules are related to the volcanic source or K/T Boundary impact ejecta found at Caribbean and Gulf of Mexico region needs detailed chemical and age characterization for which study is in progress.     

An iron isotope signature related to electron transfer between aqueous ferrous iron and goethite

We measured the Fe isotope fractionation during the reactions of Fe(II) with goethite in the presence and absence of a strong Fe(III) chelator (desferrioxamine mesylate, DFAM). All experiments were completed in an O₂-free glove box. The concentrations of aqueous Fe(II) ([Fe(II)_aq]) decreased below the initial total dissolved Fe concentrations ([Fe(II)_total], 2.15 mM) due to fast adsorption within 0.2 day. The concentration of adsorbed Fe(II) ([Fe(II)_ads]) was determined as the difference between [Fe(II)_aq] and the concentration of extracted Fe(II) in 0.5 M HCl ([Fe(II)_extr]) (i.e., [Fe(II)_ads] = [Fe(II)_extr] − [Fe(II)_aq]). [Fe(II)_ads] also decreased with time in experiments with and without DFAM, documenting that fast adsorption was accompanied by a second, slower reaction. Interestingly, [Fe(II)_extr] was always smaller than [Fe(II)_total], indicating that some Fe(II) was sequestered into a pool that is not HCl-extractable. The difference was attributed to Fe(II) incorporated into goethite structure (i.e., [Fe(II)_inc] = [Fe(II)_total] −[Fe(II)_extr]). More Fe(II) was incorporated in the presence of DFAM than in its absence at all time steps. Regardless of the presence of DFAM, both aqueous and extracted Fe(II) (δ^56/54Fe(II)_aq and δ^56/54Fe(II)_extr) became isotopically lighter than or similar to goethite (− 0.27‰) at day 7, implying that the isotope exchange occurred between bulk goethite and aqueous Fe. Consistently, the mass balance indicated that the incorporated Fe is isotopically heavier than extracted Fe. These observations suggested that (i) co-adsorption of Fe(II) with DFAM resulted in more pervasive electron transfer, (ii) the electron transfer from heavy Fe(II) in the adsorbed Fe(II) to light Fe(III) in goethite results in the fixation of heavy adsorbed Fe(III) on the surface and accumulation of Fe(II) within the goethite, and (iii) desorption of the reduced, light Fe from goethite does not necessarily occur at the same surface sites where adsorption occurred.     

The Raghunathpura iron meteorite from Alwar,India

Abstract— The Raghunathpura iron meteorite fell on 20 November 1986 around 8 p.m.; it weighed 10.2 kg. It is a group IIA hexahedrite based on metallographic, x-ray diffraction and chemical studies. Eighteen major, minor and trace elements were determined using classical, atomic-absorption spectrometry and semiquantitative spectrographic procedures.     

Effects of atmospheric boundary layer mixing representations on vertical distribution of passive and reactive tracers

Summary A one-dimensional chemistry-boundary layer model was used to study the effects of differing representations of atmospheric boundary layer (ABL) processes on simulated concentrations of passive and chemically reactive tracers. Two local- and two nonlocal-closure ABL schemes were used to perform numerical simulations during convective conditions in the ABL. Observational data from the First International Statellite Land Surface Climatology Project (ISLSCP) Field Experiment (FIFE) were used to provide initial meteorological conditions while representative chemical concentrations and surface and elevated emission rates were used to provide initial chemical conditions and chemical sources to the one-dimensional model. Two sets of numerical simulations were performed using the four ABL schemes. The first set simulated bottom-to-top mixing characteristics, and the second set simulated top-to-bottom mixing characteristics. Model simulations were performed for 12h starting from 0700LT 11 July 1987.Our analysis indicate that the simulated concentrations of both passive and reactive chemical species were sensitive to the type of ABL scheme used to represent turbulent mixing processes. Characteristic features associated with each scheme (e.g., growth and intensity of mixing in the ABL) contributed to the differences among the simulated species concentrations. For some of the chemical species these differences were large, particularly in the surface layer and in the interfacial layers of the ABL. In turn, differences caused by the differing mixing representations resulted in different chemical production/destruction rates. As a consequence, the simulated species concentrations differed among the simulations. We also found that chemical species concentrations were more sensitive to the type of ABL scheme in the bottom-to-top mixing simulations than in the top-to-bottom simulations.With 10 Figures