维生素对凡纳滨对虾N-乙酰-β-D-氨基葡萄糖苷酶的效应研究

以凡纳滨对虾中提取的N-乙酰-β-D-氨基葡萄糖苷酶(NAGase,EC 3.2.1.52)为研究对象,以对-硝基苯-N-乙酰-β-D-氨基葡萄糖苷(pNP-NAG)为底物,考察了几种养殖常用维生素对该酶活力的影响.结果表明,VB12、烟酸、核黄素等对酶活力基本上没有影响;而VB1、VB6、抗坏血酸等对该酶活力均有不同程度的抑制作用.随着抑制剂浓度增大,酶活力呈指数下降,测定导致酶活力下降50%的抑制剂浓度(IC50)分别为16.0、13.5、37.5mmol/dm3.进一步研究了VB6的抑制作用动力学,结果显示:VB6对酶的抑制作用为非竞争性可逆抑制,其对酶抑制常数(KI)为 15.2mmol/dm3.该研究对凡纳滨对虾的人工养殖具有一定的参考价值.     

The study on a new technology of waterway information system

It is a lot limitations to survey and design waterway by the CAD technique,due to timely variation of hydrology and ecology in the complicated estuary area,such as Changjing River estuary.In this paper,the advantages of GIS technique is applied to manage and process the multi kind,multi scale and multi time hydrographic parameters,including water depth,current velocity,tidal,waves,wind speed,temperature and salinity etc.,also to deal with different projection,different scale charts.Meanwhile,in the paper,combination techniques of GIS and CAD has been developed for unification of the charts for efficient management,two-way automatic carts conversion between GIS and CAD,automatic cutting and assemble of a number of Charts,flight path of the projection of spatial data and coordinate transformation.This new technique has been applied to survey and design waterway of Changing River estuary.The result shows that the combination technology of GIS and CAD has application potentiality in the waterway survey and design.     

Study on General Performance of Jack-up Under Elevated Condition

Method of checking for jack-up elevated performance including leg structure strength, fixation system or jacking system bearing capacity, pre-load requirements, spud can bearing capacity and overturning stability is suggested in this paper. As an example, a jack-up with truss legs is analyzed by finite element analysis method. This paper may be helpful to the rig owners, operators and designers.     

闽东沿岸2004～2006年浮游植物生态特征研究

根据闽东海洋环境监测中心2004年-2006年阂东沿岸生态调查的资料,分析和研究了闽东沿岸浮游植物的生态特征．共鉴定出浮游植物的种类3个门39属共134种,其中硅藻门的种类占总种数的80％,为该调查海域主要门类．主要优势种是中肋骨条藻（Skeletonema costatum）、旋链角毛藻（Chaetoceros curvisetus）、尖刺拟菱形藻（Pseudo—nitzschia pungens）和丹麦细柱藻（Leptocylindrus danicus）等．浮游植物的丰度分布,呈现由调查海域东北侧向西南侧、由近岸向远岸减少的空间特征,夏季高、春季低的季节变化特点,并且有逐年减少的年际变化趋势．本调查海域浮游植物多样性指数较高,群落较稳定．     

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西湖凹陷平北地区烃源岩特征及生排烃史

为了综合确定平北地区的主力烃源岩,深化其油气成藏动力学过程研究,在烃源岩分布特征及有机质丰度、类型和成熟度分析的基础上,应用舍油气盆地数值模拟技术,定量恢复了研究区主要烃源岩层系的生排烃历史。研究表明,平北地区主要发育始新统平湖组、渐新统花港组两套烃源岩系,其中平湖组暗色泥岩为主力烃源岩,具较高的有机质丰度、成熟度、生排烃强度与排烃效率;以平湖组为源岩的油气系统应是本区油气勘探的主要目标。     

利用ITS-5.8S rDNA序列对2株海洋亚历山大藻进行的分子鉴定

扩增2株分离自青岛胶州湾、从形态上初步确定为亚历山大藻属(Alexandrium sp. qd2, Alexandrium sp. qd3)的5.8S 核糖体基因(5.8S rDNA), 转录单元内间隔区(Internal Transcribed Spacer, ITS) 序列、部分18S rDNA和28S rDNA序列.通过blastn比对、系统进化树以及遗传距离分析将A.sp.qd2和A.sp.qd3鉴定为A.catenella,且属于A.catenella 的不同株型.