Purification and characterization of an alkaline protease from Acetes chinensis

An alkaline protease from Acetes chinensis was purified and characterized in this study. The steps of purification include ammonium sulfate precipitation, ion-exchange chromatography with Q-sepharose Fast Flow, gel filtration chromatography with S300 and the second ion-exchange chromatography with Q-sepharose Fast Flow. The protease was isolated and purified, which was present and active on protein substrates (azocasein and casein). The specific protease activity was 17.15 folds and the recovery was 4.67. The molecular weight of the protease was estimated at 23.2 kD by SDS-PAGE. With azocasein as the susbstrate, the optimal temperature was 55°C and the optimal pH value was 5.5. Ion Ca²⁺ could enhance the proteolytic activity of the protease, while Cu²⁺, EDTA and PMSF could inhibit its activity.     

多口出入的网络停车场系统设计

介绍了大型停车场系统设计实现以及安全性原理，针对现有停车场系统的缺点和不足因素，在保证传统停车场系统优势的基础上，采用先进的编程技术、硬件设计和网络技术建立适应不同停车场类型的软硬件系统，在实际应用中取得了很好的效果。     

Searching for Variable Stars in and around Open Clusters

We present a snapshot of our recent results of a variable star survey in 1 degree fields around three open clusters: NGC 188, NGC 7789 and M67. A total number of 39 variable stars are newly discovered, including 22 W UMa stars, 10 EA (Algol) type binaries, one RR-lyr and one RRd pulsator, and five unclassified variables.     

EVALUATION ON NUTRITIVE VALUE OF PORTUNUS TRITUBERCULATUS

MODUon0NP.trituberculatushasanupswellingshuttiedsecephalothoraxcrust0nWhosescheearemanydiversefinegranuleswhichbererneroughandconcentIatalinthegillaas.IthasthIeewartybulgesandtwosharpodhonitSforeheadPaiAiy-U,l986).Thechelipedisdeveloped,itSmerusisshuuleshaped,thefotirthpairofpereiopodsareuseoars,itScarpopoditiswideandshort,thedaCty0poditisslab-shaped,eachjoint'sedgehasshorthahaforswirTuning,themalebodyisbluishgreen,andthebolebodyisadeeppmple.Maleanddrileglands')locateatthedorsalordorsola…     

类氖铁(Fe XVII)离子能级及振子强度计算

利用原子数据模拟程序RCN／RCG、AUTOSTRUCTURE(AS)、GRASP分别对类氖铁的离子结构进行计算，计算过程包含了对波函数的相对论修正，计算结果包括电偶级光谱跃迁的能级间隔，谐振子强度，跃迁几率，并与实验值进行了比较，分析了3种原子数据模拟程序在计算原子结构上的精确度．     

滇东北地区地质灾害及其对公路工程影响研究——以某拟建公路沿线地质灾害为例

以滇东北地区某拟建公路沿线的地质灾害为例 ,分析研究该地区地质灾害的影响因素、影响机制、分布规律以及对公路工程的影响。区内地质灾害的发育受地层岩性、岩体结构、地质构造、地震、水文条件、气候条件、人类活动等因素的控制和影响。地质灾害类型主要有崩滑塌、滑坡、泥石流、岩溶塌陷等 ,另外还存在软土、膨胀土及可液化的砂土等特殊岩土灾害。不同类型的灾害对公路工程的影响和破坏机制不同     

2000年度上海天文台卫星激光测距观测报告

介绍了 2 0 0 0年度上海天文台卫星激光测距观测和系统的改进情况     

Fine‐grained rims in the Allan Hills 81002 and Lewis Cliff 90500 CM2 meteorites: Their origin and modification

Abstract— Antarctic CM meteorites Allan Hills (ALH) 81002 and Lewis Cliff (LEW) 90500 contain abundant fine‐grained rims (FGRs) that surround a variety of coarse‐grained objects. FGRs from both meteorites have similar compositions and petrographic features, independent of their enclosed objects. The FGRs are chemically homogeneous at the 10 μm scale for major and minor elements and at the 25 μm scale for trace elements. They display accretionary features and contain large amounts of volatiles, presumably water. They are depleted in Ca, Mn, and S but enriched in P. All FGRs show a slightly fractionated rare earth element (REE) pattern, with enrichments of Gd and Yb and depletion of Er. Gd is twice as abundant as Er. Our results indicate that those FGRs are not genetically related to their enclosed cores. They were sampled from a reservoir of homogeneously mixed dust, prior to accretion to their parent body. The rim materials subsequently experienced aqueous alteration under identical conditions. Based on their mineral, textural, and especially chemical similarities, we conclude that ALH 81002 and LEW 90500 likely have a similar or identical source.     

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Genetic variation in parthenogeneticArtemia from the Shandong Peninsula,P.R.C.

Results are presented of a survey of isozyme frequencies in parthenogeneticArtimia from six salterns in the Shandong Peninsula, P.R.C. Three of the eleven enzymes we scored using polyacryamide gel electrophoresis proved to be useful for analysis of electromorph frequency variation. These enzymes are tetrazolium oxidase (TO), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH). Each of these systems showed extensive isozyme variation both within and between salterns. In addition, we have examined the possibility that electromorph frequencies may vary as the result of natural selection for adaptation to specific salinity, or salinity-associated environmental conditions. An indication of clinal variation was found in two series of ponds differing in salinity, however more extensive data are needed before it is possible to conclude that these patterns are the result of natural selection. Finally, the use of isozyme analyses such as ours, for unraveling taxonomic problems inArtemia is discussed.