Caffeine in Boston Harbor seawater

Caffeine has been detected in Boston Harbor seawater with concentrations ranging from 140 to 1600 ng l(-1), and in Massachusetts Bay seawater at concentrations from 5.2 to 71 ng l(-1). Sources of caffeine appear to be anthropogenic with higher concentrations in the seawater of Boston's inner harbor and in freshwater sources to the harbor. Charles River water and Deer Island sewage treatment plant effluent, the two major sources of freshwater to the harbor, contained 370 and 6700 ng l(-1) of caffeine, respectively, in 1998. Sewage influent and effluent concentrations appear to be consistent with consumption estimates of caffeinated beverages for the Boston area and total organic carbon removal targets for treated sewage. Caffeine was inversely correlated to salinity in a transect from the mouth of Boston Harbor to Stellwagen Basin, indicating it may be a useful chemical tracer of anthropogenic inputs to marine systems.     

Growth and Deformation of the Ladakh Batholith, Northwest Himalayas: Implications for Timing of Continental Collision and Origin of Calc-Alkaline Batholiths

The calc-alkaline Ladakh batholith (NW Himalayas) was dated to constrain the timing of continental collision and subsequent deformation. Batholith growth ended when collision disrupted subduction of the Tethyan oceanic lithosphere, and thus the youngest magmatic pulse indirectly dates the collision. Both U-Pb ages on zircons from three samples of the Ladakh batholith and K-Ar from one subvolcanic dike sample were determined. Magmatic activity near Leh (the capital of Ladakh) occurred between 70 and 50 Ma, with the last major magmatic pulse crystallizing at ca. 49.8+/-0.8 Ma (2sigma). This was followed by rapid and generalized cooling to lower greenschist facies temperatures within a few million years, and minor dike intrusion took place at 46+/-1 Ma. Field observations, the lack of inherited prebatholith zircons, and other isotopic evidence suggest that the batholith is mantle derived with negligible crustal influence, that it evolved through input of fresh magma from the mantle and remelting of previously emplaced mantle magmatic rocks. The sedmimentary record indicates that collision in NW Himalaya occurred around 52-50 Ma. If this is so, the magmatic system driven by subduction of Tethys ended immediately on collision. The thermal history of one sample from within the Thanglasgo Shear Zone (TSZ) was determined by Ar-Ar method to constrain timing of batholith internal deformation. This is a wide dextral shear zone within the batholith, parallel to the dextral, N 30 degrees W-striking crustal-scale Karakoram Fault. Internal deformation of the batholith, taken up partly by this shear zone, has caused it to deviate from it regional WNW-ESE trend to parallel the Karakoram Fault. Microstructures and cooling history of a sample from the TSZ indicate that shearing took place before 22 Ma, implying that (1) the history of dextral shearing on NW-striking planes in northern Ladakh started at least 7 m.yr. before the <15 Ma Karakoram Fault, (2) shearing was responsible for deviation of the regional trend of the Ladakh batholith, and (3) dextral shearing occured within a zone apporximately 100 km wide that includes the Ladakh batholith and portions of the younger Karakoram batholith.     

Numerical Simulations of Oscillation Modes of the Solar Convection Zone

The ACIS front-illuminated CCDs on board the Chandra X-Ray Observatory were damaged in the extreme environment of the Earth's radiation belts, resulting in enhanced charge transfer inefficiency (CTI). This produces a row dependence in gain, event grade, and energy resolution. We model the CTI as a function of input photon energy, including the effects of detrapping (charge trailing), shielding within an event (charge in the leading pixels of the 3x3 event island protects the rest of the island by filling traps), and nonuniform spatial distribution of traps. This technique cannot fully recover the degraded energy resolution, but it reduces the position dependence of gain and grade distributions. By correcting the grade distributions as well as the event amplitudes, we can improve the instrument's quantum efficiency. We outline our model for CTI correction and discuss how the corrector can improve astrophysical results derived from ACIS data.     

Titan's 5 micrometers spectral window: carbon monoxide and the albedo of the surface

We have measured the spectrum of Titan near 5 micrometers and have found it to be dominated by absorption from the carbon monoxide 1-0 vibration-rotation band. The position of the band edge allows us to constrain the abundance of CO in the atmosphere and/or the location of the reflecting layer in the atmosphere. In the most likely case, 5 micrometers radiation is reflected from the surface and the mole fraction of CO in the atmosphere is qCO=10(+10/-5) ppm, significantly lower than previous estimates for tropospheric CO. The albedo of the reflecting layer is approximately 0.07(+0.02/-0.01) in the 5 micrometers continuum outside the CO band. The 5 micrometers albedo is consistent with a surface of mixed ice and silicates similar to the icy Galilean satellites. Organic solids formed in simulated Titan conditions can also produce similar albedos at 5 micrometers.     

Growth of estuarine fish is associated with the combined concentration of sediment contaminants and shows no adaptation or acclimation to past conditions

We tested whether the growth rates of small benthic fish (Gillichthys mirabilis) in three southern California estuaries corresponded with the local concentrations of contaminants. Fish originating from each estuary were transplanted to cages in each estuary in two reciprocal transplant experiments. The growth rates of caged fish, and the size-distribution of natural populations, showed the same pattern of difference among estuaries. Twelve metals and organic contaminants occurred in bulk sediments at concentrations close to their individual ERL values, and a simple index of their combined concentration (the mean ERL quotient) was inversely correlated to the growth of caged fish. Metals in the water column occurred at lower concentrations, relative to toxicity thresholds, than those in sediments and were unrelated to fish growth. Fish used in the field caging experiments, and other fish held in the laboratory under constant conditions, showed no difference in growth according to their estuary of origin. Fish originating from different estuaries also showed no consistent differences in their tissue burden of organic contaminants. Our results thus suggested no genetic adaptation or physiological acclimation to the past contaminant regime, but revealed a possible association between fish growth rates and the combined concentration of multiple sediment contaminants.     

DNA strand breaks in grass shrimp embryos exposed to highway runoff sediments and sediments with coal fly ash

Embryo production was reduced in female grass shrimp exposed to sediments with added coal fly ash and to sediments collected from an estuarine station containing high PAH concentrations due to its proximity to a highway storm drain. Grass shrimp embryos exposed to pore water from the high PAH and high metal sediments showed both reduced hatching and increases in DNA strand breaks (comet assay). Sediments with added coal fly ash had high concentrations of vanadium and selenium which may have contributed to effects similar to those observed with sediments with high PAH. The embryo pore water bioassay (hatching/DNA strand breaks) gave results comparable to those observed for reproduction effects (reduced embryo production/embryo hatching) with female grass shrimp exposed to whole sediment.     

Effects of genotoxic compounds on DNA and development of early and late grass shrimp embryo stages

Early and late developmental stages of grass shrimp embryos were exposed to different concentrations of two genotoxicants, 2-methyl-1,4-naphthoquinone (MNQ) and 4-nitroquinoline-N-oxide (NQO). DNA strand breaks were assessed by the comet assay while embryo development effects were determined by % of embryos hatching. Early embryo stage embryos were significantly more sensitive to genotoxicants than late stages. For example, all stage 4 embryos failed to hatch at 1 microM NQO while 95% of stage 8 hatched at this concentration. High DNA tail moments, which are a measure of the number of DNA strand breaks, were found in late stage embryos exposed to genotoxicants. Early stage embryo development was effected by low concentrations of genotoxicants but no changes were observed in DNA tail moments. We suggest that high DNA moments in late embryo stages reflect high DNA repair activity, while early stages may lack a fully developed DNA repair system.