浮游植物化学分类法—CHEMTAX运算在南中国海应用的改进和优化

In order to determine the phytoplankton community composition, the modification and optimizing of the CHEMical TAXonomy (CHEMTAX) running was carried out through samples grouping, successive run and ev...     

基于四种水产病原弧菌的简型芯片检测体系的优化

基因芯片技术在病原菌检测领域展现出良好的前景,本文依托水产病原菌基因检测芯片的检测体系,以水产常见病原弧菌的hsp60、toxR基因检测探针为研究对象,系统研究不同点样液配方、寡核苷酸探针浓度、荧光标记物质浓度、杂交温度、杂交时长对芯片杂交效果的影响,并进行相应优化。结果表明,杂交温度为60℃、杂交时长为2h、探针浓度为1μmol/L、Cy3标记的随机引物的用量为150pmol时,芯片的经济性、灵敏度、检测效率达到均衡;以0.1mol/L磷酸氢二钠和25%二甲基亚砜混合溶液作为点样液,效果较好。优化后的芯片对4种弧菌的检测没有出现交叉杂交现象,探针特异性达到100%;对溶藻弧菌全基因组DNA检测灵敏度为10fg,高出PCR方法约2个数量级。     

大万山波浪能试验场泊位间水动力影响分析

当前我国近海海域属稀缺资源,波浪能试验场建设须通过合理布局各测试泊位,在保证试验场各泊位可同时正常开展测试工作的前提下,尽量减小用海面积。拉近试验泊位距离,是减小用海面积的直接手段,但在此过程中,必须保证在试验场场址海域水动力环境下,泊位接入测试波浪能装置后,其之间的水动力影响在可接受的范围内。以大万山波浪能试验场泊位设计为例,介绍一种有效的泊位间水动力影响分析方法。方法采用自主开发的二维Boussinesq波浪模型,开展水动力影响分析工作,同时引入相对波高比概念,对泊位接入装置后影响距离和影响面积进行了定量的分析。文中研究得出的分析方法,可用于判定泊位布局设计的合理性。     

Ultimate Load Capacity of Offshore Pipeline with Arbitrary Shape Corrosion Defects

A set of generalized solutions are proposed for estimating ultimate load capacity of pipeline with arbitrary corrosion shapes subjected to combined internal pressure, axial force and bending moment. Isotropic and anisotropic material characteristics in longitudinal and circumferential direction of pipeline are also considered in the proposed equations. Simplified numerical method is used to solve the generalized expressions. The comparisons of numerical results based generalized solutions and full-scale experimental results are carried out. The predicted results agree reasonably well with the experiment results. Meanwhile, the effects of corrosion shapes and locations on the ultimate load capacity are studied.     

冲绳海槽中部热液区及典型喷口区地形地貌特征

利用最新测量的高精度深水多波束资料,对冲绳海槽中部热液活动区的地形地貌特征进行了系统的分析后发现,研究区地形情况相当复杂,起伏变化较大,总体上呈沿SW—NE向延伸的裂谷地势,裂谷水深范围约在1 500~1 800m,大量呈SW—NE向的线性海山链和裂谷、洼地地貌交错出现,分析认为其形成可能与冲绳海槽的弧后扩张作用有关。另外,通过ROV(Remotely Operated Vehicle)上获得的影像,结合浅表层沉积物和岩石样品分析发现,2处喷口地形地貌情况明显不同,Iheya North喷口区地形起伏变化大,具有较多烟囱体、丘体,周围沉积物以硫化物砂为主;Iheya Ridge则以裂隙式溢流为主,分布范围广,地形倾斜平整,少见烟囱体、丘体,底质较硬,热液沉积物分布较少。     

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Abundance and Distribution of Fatty Acids in Sediments of the South Mid-Atlantic Ridge

Sediment samples obtained from the South Mid-Atlantic Ridge were studies by gas chromatography-mass spectrometer(GC-MS) for the abundance and distributions of total fatty acids(TFAs). Approximately 34 fatty acids were identified, with the chain-lengths ranging from C12 to C30. The total concentrations of TFAs(∑TFA) ranged from 7.15 to 30.09 μg g-1 dry sediment, and ∑TFA was weakly correlated with bitumen content(R2 = 0.69). The ∑TFA of samples around hydrothermal areas were significantly higher than that of samples away from hydrothermal areas, indicating intense primary production and large biomass in the hydrothermal areas, and suggesting a close relationship between hydrothermal activity and ∑TFA of samples. The characteristics of the TFA composition in the present study are rich in monounsaturated fatty acids and lacking in polyunsaturated fatty acids, and the ratios between the concentrations of monounsaturated fatty acids and ΣTFAs in samples close to the hydrothermal areas, are about 0.8, but for samples far from the hydrothermal areas, they are only about 0.5. Several fatty acids(e.g., a/i C15:0 and C16:1ω7), which are signature biomarkers for sulfur-metabolizing bacteria, show the same distribution trend as ∑TFA of samples, further highlighting the close relationship between fatty acid content and hydrothermal activity and/or hydrothermal communities. The metabolic activities of hydrothermal communities, especially those of microorganisms, are likely the main source of fatty acids in samples.     

地质勘查一线紧缺人才数据库支撑平台建设探析

运用B/S（浏览器/服务器）模式和OOAD(面向对象的分析和设计)等信息技术,建设地质勘查一线紧缺人才数据库支撑平台,实现人才数据采集、培养计划和培养目标的管理,以及相关数据查询、统计功能,全面、准确、动态掌握地质勘查一线紧缺人才培养全过程,为搭建地质勘查一线紧缺人才信息服务平台奠定基础,同时为国土资源管理决策工作提供依据。     

多尺度GTWR城市住宅价格建模与分析

尺度、时间、空间距离一直是制约地理时空加权回归模型求解精度的关键.本文基于欧氏距离约束和路网距离约束,将多尺度扩展到时空地理加权回归方法的建模中,以检验多尺度GTWR模型的改进性能,同时验证路网距离约束在多尺度GTWR模型中的优越性.以2015—2018年成都市主城区商品房社区作为案例对象,将多尺度GTWR与GTWR在拟合优度(R2)、残差平方和(RSS)及AIC等方面进行比较.试验结果表明,与GTWR相比,多尺度GTWR对影响住宅价格的自变量提供了更有效的解释,同时路网距离的使用也提高了模型的合理性.在基于欧氏距离约束和路网距离约束方面拟合优度分别提升了0.123和0.208,RSS和AIC值得到了有效的降低.相比于使用欧氏距离约束的GTWR与多尺度GTWR模型,路网距离约束的GTWR(RD)模型的拟合优度提高了0.007,多尺度GTWR(RD)模型的拟合优度提高了0.092,基于路网距离的计算结果进一步证实了多尺度GTWR模型的正确性,也进一步证明了综合考虑尺度、时空距离后的多尺度GTWR具有很好的通用性.     

基于层次规则的OSM建筑物面目标冗余清理

开放街道地图(Open Street Map,OSM)数据由大众自发标报,因而存在大量虚假、低质量、冗余数据,其冗余数据清理研究成果乏善可陈.为此,针对OSM建筑物面目标冗余问题,构建了一套基于层次规则的OSM建筑物面目标冗余数据清理模型.首先,从OSM数据中识别冗余建筑物面目标,然后,按照拓扑关系类型将冗余建筑物面目标进行冗余分类,其次,根据建筑物面目标的冗余类型、几何特征等构建层次规则,最后,对实验区冗余面目标进行实验,并利用精确率、召回率及调和平均值(F1)等指标评价清理效果,结果表明,该模型具有良好的合理性和有效性.