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151.
羟化酶是细菌降解烷烃的关键酶,本文以南极低温降解菌Shewanella sp.NJ49为研究对象,通过硫酸铵沉淀、超滤浓缩及葡聚糖凝胶Sephadex G-75柱层析方法,分离纯化NJ49的羟化酶,获得了单一羟化酶组分,研究了温度、p H、表面活性剂和盐度等环境因素对NJ49羟化酶酶活活性影响。结果表明,羟化酶组分由α、β、γ三个亚基组成,分子质量分别为56,45和36 k D。NJ49最适产酶条件为:温度15℃、p H7.2、盐度55;不同类型表面活性剂对酶的活性影响效应不一,两性离子表面活性剂、阳离子表面活性剂、非离子表面活性剂对酶活没有作用,阴离子表面活性剂+非离子表面活性剂混和剂和阳离子表面活性剂+非离子表面活性剂混和剂能提高羟化酶的活性。 相似文献
152.
渤海湾曹妃甸深槽海区地形地貌特征及控制因素 总被引:2,自引:2,他引:0
通过研究浅层地震剖面、侧扫声纳和水深地形等数据资料,得出,曹妃甸沙岛的岬角地貌引起深槽海域局部潮流流速增大,甸头前沿深槽区以冲刷为主,最大水深达42 m,刷新了渤海湾最大水深记录,深槽部位的侵蚀量最大,深槽南坡冲刷幅度大于北坡,工程建设后期深槽区侵蚀冲刷程度有变小变缓趋势。早期深槽的形成是由于浅部断层受深部构造影响发生阶梯状错断沉陷,海底地层形成古凹槽,但深槽海底地层沉陷速率略大于沉积速率,使得深槽海域长期保持了渤海湾最大的水深环境。初步得出在历史时期曹妃甸深槽经过2万a以上长期存在,深槽的走向经历了南北向-北东向-北西向的转化过程。认为地质构造、古滦河三角洲演变、海洋水动力作用和人类活动等内外营力作用共同控制了曹妃甸海区地貌体系的发育与演化。达到了研究渤海湾曹妃甸深槽海区地形地貌控制因素和深槽的地质演化的目的,为曹妃甸港的规划、运营期维护和未来发展提供科学依据具有重要意义。 相似文献
153.
夏季辽东湾西部海域大型底栖动物群落结构与多样性 总被引:1,自引:0,他引:1
为了解夏季辽东湾西部海域大型底栖动物群落结构与多样性,作者于2009年8月对六股河口外海域15个站位进行了大型底栖动物调查。整个研究海域共采到大型底栖动物124种,其总平均丰度为(2824±1537)个/m2,总平均生物量为(22.01±38.58)g/m2。利用相对重要性指数和优势度指数所确定的前三位优势种一致,分别为二齿半尖额涟虫(Hemileucon bidentatus)、深钩毛虫(Sigambra bassi)和独指虫(Aricidea fragilis),其余优势种存在细微差异。各站位香农-维纳多样性指数、Pielou均匀度指数和Margalef丰富度指数均呈现河口和中部海域低,近岸和外围海域高的分布规律。在50%相似度水平上,可将研究海域划分为4个群落。研究结果表明:研究海域底栖环境整体清洁,底层水环境因子对大型底栖动物群落的影响较弱。 相似文献
154.
155.
Multiobjective optimization deals with mathematical optimization problems where two or more objective functions (cost functions) are to be optimized (maximized or minimized) simultaneously. In most cases of interest, the objective functions are in conflict, i.e., there does not exist a decision (design) vector (vector of optimization variables) at which every objective function takes on its optimal value. The solution of a multiobjective problem is commonly defined as a Pareto front, and any decision vector which maps to a point on the Pareto front is said to be Pareto optimal. We present an original derivation of an analytical expression for the steepest descent direction for multiobjective optimization for the case of two objectives. This leads to an algorithm which can be applied to obtain Pareto optimal points or, equivalently, points on the Pareto front when the problem is the minimization of two conflicting objectives. The method is in effect a generalization of the steepest descent algorithm for minimizing a single objective function. The steepest-descent multiobjective optimization algorithm is applied to obtain optimal well controls for two example problems where the two conflicting objectives are the maximization of the life-cycle (long-term) net-present-value (NPV) and the maximization of the short-term NPV. The results strongly suggest the multiobjective steepest-descent (MOSD) algorithm is more efficient than competing multiobjective optimization algorithms. 相似文献
156.
Molybdenum Mass Fractions and Isotopic Compositions of International Geological Reference Materials 下载免费PDF全文
Pei‐Pei Zhao Jie Li Le Zhang Zhi‐Bing Wang De‐Xin Kong Jin‐Long Ma Gang‐Jian Wei Ji‐Feng Xu 《Geostandards and Geoanalytical Research》2016,40(2):217-226
The double‐spike method with multi‐collector inductively coupled plasma‐mass spectrometry was used to measure the Mo mass fractions and isotopic compositions of a set of geological reference materials including the mineral molybdenite, seawater, coral, as well as igneous and sedimentary rocks. The long‐term reproducibility of the Mo isotopic measurements, based on two‐year analyses of NIST SRM 3134 reference solutions and seawater samples, was ≤ 0.07‰ (two standard deviations, 2s, n = 167) for δ98/95Mo. Accuracy was evaluated by analyses of Atlantic seawater, which yielded a mean δ98/95Mo of 2.03 ± 0.06‰ (2s, n = 30, relative to NIST SRM 3134 = 0‰) and mass fraction of 0.0104 ± 0.0006 μg g?1 (2s, n = 30), which is indistinguishable from seawater samples taken world‐wide and measured in other laboratories. The comprehensive data set presented in this study serves as a reference for quality assurance and interlaboratory comparison of high‐precision Mo mass fractions and isotopic compositions. 相似文献
157.
Yinian Zhu Bin Huang Zongqiang Zhu Huili Liu Yanhua Huang Xin Zhao Meina Liang 《Geochemical transactions》2016,17(1):2
Background
The interaction between Ca-HAP and Pb2+ solution can result in the formation of a hydroxyapatite–hydroxypyromorphite solid solution [(PbxCa1?x)5(PO4)3(OH)], which can greatly affect the transport and distribution of toxic Pb in water, rock and soil. Therefore, it’s necessary to know the physicochemical properties of (PbxCa1?x)5(PO4)3(OH), predominantly its thermodynamic solubility and stability in aqueous solution. Nevertheless, no experiment on the dissolution and related thermodynamic data has been reported.Results
Dissolution of the hydroxypyromorphite–hydroxyapatite solid solution [(PbxCa1?x)5(PO4)3(OH)] in aqueous solution at 25 °C was experimentally studied. The aqueous concentrations were greatly affected by the Pb/(Pb + Ca) molar ratios (XPb) of the solids. For the solids with high XPb [(Pb0.89Ca0.11)5(PO4)3OH], the aqueous Pb2+ concentrations increased rapidly with time and reached a peak value after 240–720 h dissolution, and then decreased gradually and reached a stable state after 5040 h dissolution. For the solids with low XPb (0.00–0.80), the aqueous Pb2+ concentrations increased quickly with time and reached a peak value after 1–12 h dissolution, and then decreased gradually and attained a stable state after 720–2160 h dissolution.Conclusions
The dissolution process of the solids with high XPb (0.89–1.00) was different from that of the solids with low XPb (0.00–0.80). The average K sp values were estimated to be 10?80.77±0.20 (10?80.57–10?80.96) for hydroxypyromorphite [Pb5(PO4)3OH] and 10?58.38±0.07 (10?58.31–10?58.46) for calcium hydroxyapatite [Ca5(PO4)3OH]. The Gibbs free energies of formation (ΔG f o ) were determined to be ?3796.71 and ?6314.63 kJ/mol, respectively. The solubility decreased with the increasing Pb/(Pb + Ca) molar ratios (XPb) of (PbxCa1?x)5(PO4)3(OH). For the dissolution at 25 °C with an initial pH of 2.00, the experimental data plotted on the Lippmann diagram showed that the solid solution (PbxCa1?x)5(PO4)3(OH) dissolved stoichiometrically at the early stage of dissolution and moved gradually up to the Lippmann solutus curve and the saturation curve for Pb5(PO4)3OH, and then the data points moved along the Lippmann solutus curve from right to left. The Pb-rich (PbxCa1?x)5(PO4)3(OH) was in equilibrium with the Ca-rich aqueous solution.158.
为了更好地认识21世纪海上丝绸之路(简称"海上丝路"),文章对"海上丝路"涉及的重要航线、节点及港口的地理位置、气候状况、水文特征、商业价值、人文背景等进行梳理。重要航线、节点及港口的使用与管理作为"海上丝路"建设的基础要素,是与造船能力并列的核心资源,三者之间环环相扣,构成"海上丝路"健康运营的完整体系。与沿"海上丝路"国家稳步推进航线、节点及港口的管理、开发与维护,打造坚实的战略支撑点、现代之"海上驿站",是成功实现我国资源多样化、产业走出去、贸易全球化的最佳路径之一,以此为契机,融入现有国际海洋法公约并在践行过程中制定利我的海上政策体制,助力"海之梦"、"中国梦"。 相似文献
159.
Currently, there is limited literature dealing with the length of stay of circular migrants in urban areas (LSCMU), although many studies have discussed the phenomenon of migration and the determinants of migration. This study attempts to fill that gap using survey data from the mountainous areas of Chongqing, Southwest China. From a comparative perspective, this study divided workers into two groups (a young group aged between 16 and 35 years and an old group aged between 36 and 65 years). The average LSCMU values for the young and old groups were 225 days and 174 days, respectively. Two multi-regression models were used to estimate the determinants of LSCMU in the two groups. The results showed that LSCMU was closely related to individual factors in both groups, including gender, age and job training. Family and community factors, including household size, arable land per capita and the distance from market, had much weaker effects on the dependent variable, especially in the older group. It was noticeable that job training had significant positive effects on LSCMU in both groups; these findings have special implications for the urbanization process in China. 相似文献
160.
对湖北大洪山地区一套紫红色砂-砾岩系沉积年代的再认识——碎屑锆石U-Pb年龄及其地质意义 总被引:1,自引:0,他引:1
湖北大洪山地区花山群六房咀组之上不整合覆盖了一套紫红色砂-砾岩。这套岩石的沉积时代存在争议,一种观点认为是南华纪早期;另一种观点认为是晚侏罗世。利用LA-ICP-MS分析技术,对这套碎屑沉积岩进行了碎屑锆石U-Pb测年,获得有效年龄数据125组,年龄值变化范围较大(3223~771Ma),主要集中于新太古代末—古元古代早期(约2500Ma)、古元古代(约2000Ma)和新元古代(约800Ma)3个时间段。对比鄂东南地区和三峡地区莲沱组岩石学特征和碎屑锆石年龄分布特征,认为其具有相似的物源,这套岩系沉积时代应该为南华纪早期而非晚侏罗世。综合分析扬子地块的前寒武纪年龄,认为扬子地块可能存在大量未岀露的新太古代末—古元古代早期(约2500Ma)基底,约2500Ma的岩石应该是扬子地块深部基底的重要组成部分。扬子地块存在广泛的古元古代(约2000Ma)岩浆活动,这期岩浆活动可能是Columbia超大陆聚合在扬子地块的响应。新元古代(约800Ma)扬子地块北缘存在Rodinia超大陆裂解同期裂谷,裂谷内基性岩可能为大洪山地区南华纪地层中基性岩砾石的物源。 相似文献