Study on biogenic amines in various dry salted fish consumed in China

This study was carried out to investigate the biogenic amines（BAs）, physicochemical property and microorganisms in dry salted fish, a traditional aquatic food consumed in China. Forty three samples of dry salted fish were gathered from retail and wholesale markets and manufacturers, which had been produced in various regions in China. Cadaverine（CAD） and putrescine（PUT） were quantitatively the most common biogenic amines. About 14% of the samples exceeded the histamine content standards established by the FDA and/or EU. The highest histamine content was found in Silver pomfret（Pampus argenteus）(347.79 mg kg^-1). Five of forty three samples exceeded the acceptable content of TYR(100 mg kg^-1), and 23.26% of dried-salted fish contained high contents of biogenic amines(above 600 mg kg^-1). In addition, species, regions, pickling processes and drying methods made the physicochemical property, microorganisms and biogenic amines in dry salted fish to be different to some extents. The total plate count（TPC） was much higher than that of total halophilic bacteria in all samples. The biogenic amines, physicochemical property and microbiological counts exhibited large variations among samples. Furthermore, no significant correlation between biogenic amines and physicochemical property and TPC was observed. This study indicated that dry salted fish may still present healthy risk for BAs, depending on the processing methods, storage conditions among others.     

Rank-size distributions of Chinese cities: Macro and micro patterns

A large number of studies have been conducted to find a better fit for city rank-size distributions in different countries. Many theoretical curves have been proposed, but no consensus has been reached. This study argues for the importance of examining city rank-size distribution across different city size scales. In addition to focusing on macro patterns, this study examines the micro patterns of city rank-size distributions in China. A moving window method is developed to detect rank-size distributions of cities in different sizes incrementally. The results show that micro patterns of the actual city rank-size distributions in China are much more complex than those suggested by the three theoretical distributions examined(Pareto, quadratic, and q-exponential distributions). City size distributions present persistent discontinuities. Large cities are more evenly distributed than small cities and than that predicted by Zipf′s law. In addition, the trend is becoming more pronounced over time. Medium-sized cities became evenly distributed first and then unevenly distributed thereafter. The rank-size distributions of small cities are relatively consistent. While the three theoretical distributions examined in this study all have the ability to detect the overall dynamics of city rank-size distributions, the actual macro distribution may be composed of a combination of the three theoretical distributions.     

Experimental study of the mechanical properties of expansive soil with added nanomaterials

The main purpose of this paper is to describe ways to improve the microstructure of expansive soil by adding nanomaterials. Mechanical tests were done to explore the changes in shear strength and compression index of expansive soil that was modified by adding different amounts of two kinds of nanomaterials (nano-alumina and nano-silica). The test results show that adding 1.2% nano-alumina and about 2% nano-silica to expansive soil provides the optimal compression index. The test results show that adding 1.2% nano-alumina and about 1.5% nano-silica to expansive soil provides the optimal unconfined compression stress. Scanning electron microscopy of the microstructure of expansive soil modified by nanomaterials provided a deeper understanding of the effects of nanomaterials on improving expansive soil.     

A model for the representation of emergency cases

The aim of this paper is to propose a location model of earthquake emergency service depot on the basis of hybrid multi-attribute decision-making method. The advantage of the proposed method is that practical mixed uncertainty of location decision information is considered, and the corresponding factors that affect the location of transfer stations are contained. To solve the location problem, a hybrid multi-attribute decision procedure without information transformation is developed. Besides, a novel weighting method and aggregation process is given. Finally, a numerical example is provided to show the feasibility and validity of the proposed method.     

Use of remote sensing in characterization and management of Dhamni micro-watershed of Chandrapur district of Maharashtra

Soil resource characterization of Dhamni micro-watershed in Chandrapur district of Maharashtra was carried out using IRS-1D LISS-III data in conjunction with field survey and ancillary data. The study indicates that nearly 84.2 per cent of the total geographical area of the watershed is under cultivation. Forest (mainly degraded) occupy only 4.5 per cent area Whereas wasteland with scrub cover 9.4 per cent area of the watershed. Nine soil series were tentatively identified and mapped as soil series association in to five mapping units. These soils belong to order Inceptisol, Vertisol and Mollisol. Except the soils of wasteland with scrub, other soils are moderately suitable for pigeonpea and soybean and have average to good productivity.     

Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.     

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.     

Comets,Enceladus and panspermia

A growing body of evidence suggests the operation of life and life processes in comets as well in larger icy bodies in the solar system including Enceladus. Attempts to interpret such data without invoking active biology are beginning to look weak and flawed. The emerging new paradigm is that life is a cosmic phenomenon as proposed by Hoyle and Wickramasinghe (Lifecloud: the Origin of Life in the Galaxy, 1978) and first supported by astronomical spectroscopy (Wickramasinghe and Allen, Nature 287:518, 1980; Allen and Wickramasinghe, Nature 294:239, 1981; Wickramasinghe and Allen, Nature 323:44, 1986). Comets are the transporters and amplifiers of microbial life throughout the Universe and are also, according to this point of view, the carriers of viruses that contribute to the continued evolution of life. Comets brought life to Earth 4.2 billion years ago and they continue to do so. Space extrapolations of comets, Enceladus and possibly Pluto supports this point of view. Impacts of asteroids and comets on the Earth as well as on other planetary bodies leads to the ejection of life-bearing dust and rocks and a mixing of microbiota on a planetary scale and on an even wider galactic scale. It appears inevitable that the entire galaxy will be a single connected biosphere.