一株深海耐嗜压菌的细胞毒活性次生代谢产物研究

通过CCK8法检测分离自深海一株深海耐压菌Shewanella piezotolerans WP3次生代谢产物的细胞毒活性,研究了温度、压力、噬菌体对WP3次生代谢产物细胞毒活性的影响.发现WP3次生代谢产物具有良好细胞毒活性.低温因素可提高其细胞毒活性,在100μg/cm^3的含量下,代谢产物对肿瘤细胞Bel7402、CNE2、A549、HT1080细胞毒活性均有提高,其中对A549细胞毒活性提高了1.3倍.在最适温度（20℃）情况下,高压可降低其细胞毒活性.20 MPa培养条件下,代谢产物对4株肿瘤细胞均未表现出明显活性,而常压培养条件下对SW 480具有93.3%的抑制率,对HT1080抑制率表现出87.5%,对CNE2抑制率是79.8%.在低温情况下,压力因素对其细胞毒活性无影响,噬菌体对细胞毒活性影响不大.HPLC指纹图谱分析发现不同温度条件下,其次生代谢产物指纹图谱有变化,结合细胞毒活性结果分析,为后期分离鉴定细胞毒活性化合物提供指导.     

黄渤海松江鲈鱼线粒体控制区结构与序列多态性分析

测定了辽宁丹东、盘锦与河北秦皇岛35尾松江鲈鱼线粒体控制区序列977bp。共检出单倍型30个,多态位点35个,简约信息位点27个,绝大多数变异集中在192-342bp、594-783bp区段,同时识别出了线粒体控制区5’端终止序列区、中央保守区和3’端保守序列区的关键序列。3个群体总的单倍型多样性为0.990,核苷酸多样性为0.00718,丹东群体核苷酸多样性最高(0.00959)。群体间、群体内平均遗传距离低(分别为0.0067和0.0063);在NJ树上不同地理来源的个体混杂分布;Fst分析显示丹东与秦皇岛群体间遗传分化相对较大(0.14222,p0.05),其余不明显(0.02865-0.05616,p0.1),表明3个群体有可能隶属同一个种群。核苷酸不配对分布图表明松江鲈鱼可能未经历过大规模的种群扩张。     

韩国“新北方政策”对接“一带一路”倡议：机遇与挑战

韩国“新北方政策”与“一带一路”倡议的对接合作为中韩两国带来了重要发展机遇。文章主要探讨韩国“新北方政策”的内涵及其与“一带一路”倡议对接的进展、机遇与挑战,得出双方对接的合作机遇主要在政策沟通、设施联通、贸易投资和资金融通4个方面,同时也存在着制度性风险、地缘风险和投资合作风险的三大挑战。在具体对接过程中,产业、金融、物流3个领域可能成为突破口。     

平阴县土壤中重金属元素异常成因研究

通过对平阴县周边及其西部农田区采样调查,平阴县周边元素异常具有异常衬度较高,富集系数大等特征,西部农田区异常具有各元素套合关系好,多数异常衬度不高,富集系数小的特点。结果表明,西部农田区异常是以As,Cd为主要成分的异常,元素含量与SiO2,Al2O3,Fe2O3等常量组分间具有显著的相关性,随SiO2含量的增加而降低,随Al2O3,Fe2O3含量的增加而增加,说明土壤常量组分及其所决定的土壤质地是导致异常形成的主要因素;而平阴城区异常是以S,Hg,As,Zn,Mn等元素为主的异常,是典型的人为综合因素（化工烟尘、工厂污水、生活垃圾）产生的异常。     

Adaptive output feedback control based on DRFNN for AUV

The tracking control problem of AUV in six degrees-of-freedom (DOF) is addressed in this paper. In general, the velocities of the vehicles are very difficult to be accurately measured, which causes full state feedback scheme to be not feasible. Hence, an adaptive output feedback controller based on dynamic recurrent fuzzy neural network (DRFNN) is proposed, in which the location information is only needed for controller design. The DRFNN is used to online estimate the dynamic uncertain nonlinear mapping. Compared to the conventional neural network, DRFNN can clearly improve the tracking performance of AUV due to its less inputs and stronger memory features. The restricting condition for the estimation of the external disturbances and network's approximation errors, which is often given in the existing literatures, is broken in this paper. The stability analysis is given by Lyapunov theorem. Simulations illustrate the effectiveness of the proposed control scheme.     

4种微生态制剂对对虾育苗水体主要水质指标的影响

研究了由球形红假单胞菌(Rhodopseudomlonas sphaeroides)、噬菌蛭弧菌(Bdellovibrio bacteriovorus)及黏红酵母(Rhodotorula glutinis)制成的4种微生态制剂对中国对虾(Fenneropenaeus chinensis)育苗水质的影响.结果表明,试验组与对照组相比都能明显净化水质,试验组中以添加噬菌蛭弧菌和黏红酵母组的效果最差,添加球形红假单胞茵和噬茵蛭弧茵组的效果最好,此组水质的pH值稳定,NH4+-N明显下降,而亚硝酸盐、化学需氧量及硫化物等指标也优于其他试验组.统计分析显示,试验组与对照组相比,细菌数降低了3个数量级,添加嗜菌蛭弧菌试验组在减少异养茵(包含有害细菌)方面效果较好.同时用鳗弧菌攻毒,在5 d内,对照组的死亡率显著高于试验组(P<0.05),试验组免疫保护率为29.4%～58.5%,以添加嗜菌蛭弧菌和球形红假单胞菌组最高.建议联合使用噬菌蛭孤菌和球形红假单胞菌,混合密度初步定为2×105个/mL.     

Isolation and identification of bacteria associated with the surfaces of several algal species

We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on ...     

Laboratory Experiments on an Internal Solitary Wave over a Triangular Barrier

Laboratory experiments were conducted to investigate the evolution of interfacial internal solitary waves(ISWs) incident on a triangular barrier. ISWs with different amplitudes were generated by gravitational collapse. The ISW energy dissipation and turbulence processes were calculated as waves passed over the triangular barrier. Experimental results showed that ISWs were reflecting back off the triangular barrier, and shoaling ISWs led to wave breaking and mixing when waves propagated over the obstacle. Wave instability created the dissipation of energy as it was transmitted from waves to turbulence. The rate of ISW energy dissipation, the maximum turbulent dissipation, and the buoyancy diffusivity linearly increased with the increase in the incident wave energy.     

Status of the Recent Declining of Arctic Sea Ice Studies

In the past 30 years, a large-scale change occurred in the Arctic climatic system, which had never been observed before 1980s. At the same time, the Arctic sea ice experienced a special evolution with more and more rapidly dramatic declining. In this circumstance, the Arctic sea ice became a new focus of the Arctic research. The recent advancements about abrupt change of the Arctic sea ice are reviewed in this paper .The previous analyses have demonstrated the accelerated declining trend of Arctic sea ice extent in the past 30 years, based on in-situ and satellite-based observations of atmosphere, as well as the results of global and regional climate simulations. Especially in summer, the rate of decrease for the ice extents was above 10% per decade. In present paper, the evolution characteristics of the arctic sea ice and its possible cause are discussed in three aspects, i.e. the sea ice physical properties, the interaction process of sea ice, ocean and atmosphere and its response and feedback mechanism to global and arctic climate system.     

神经网络油气检测技术

多参数神经网络油气检测技术首先提取四大类２６ 个地震波特征参数,并定量描述这些参数,然后用神经网络技术对这些参数进行分析,预测油气分布,使油气识别工作定量化、计算机化。本次研究开发了一套神经网络油气识别软件,并利用其对东濮凹陷胡１９ 块进行油藏精细描述,预测沙三下砂组含油面积０－７ ｋｍ２ ,石油地质储量７０ ×１０４ 吨。