STPIR和MW组合的北斗三频周跳探测与修复

针对载波相位观测值中出现周跳的问题及北斗卫星导航系统全星座播发三频信号的现状,提出一种三频相位电离层残差二阶历元差分(STPIR)的算法,克服了传统电离层残差法受观测数据采样间隔影响较大的问题,联合MW组合观测量进行周跳探测又可避免各自的探测盲区。两种组合观测量均很好地削弱了电离层延迟项的影响,联立方程组进行周跳求解时,直接取整即可得到周跳值。通过北斗三频实测数据验证,提出的组合方法在观测数据采样间隔较大时,可以准确探测出所有周跳,并有效修复。     

末次间冰期以来对宁黄土剖面的热释光测年

利用细颗粒热释光技术对陇西黄土高原会宁黄土剖面末次间冰期以来黄土、古土壤的8个样品进行了年龄测定,并在此基础上分别利用线性内插和多项式回归方法建立了会宁剖面深度-年代时间模式,以2种模式计算的S0/L1、L1/S1、S1/L2界线年龄与SPECMAP深海氧同位素1/2、4/5、5/6阶段的界线年龄可较好对比,这为末次间冰期以来会宁地区古气候演化的深入研究提供了基本时间框架。     

A Preliminary Genetic Linkage Map of Sinonovacula constricta(Lamarck, 1818) Based on Microsatellites Derived from RAD Sequencing

Sinonovacula constricta is one of the important economic aquaculture species in China. In this study, we constructed genetic linkage maps of S. constricta based on 300 microsatellite markers derived from RAD-seq using an F1 full-sib family. The female map contained 204 microsatellites assigned to 22 linkage groups, which covered 1529.5 cM with an average interval of 10.3 cM. The male consisted of 187 microsatellites in 19 linkage groups corresponding to the haploid chromosome number(n(28)19), which spanned 1429.3 cM with an average interval of 8.7 cM. The genome coverage was approximately 83.5% and 81.4%, respectively. An integrated map was constructed according to the common markers in parental linkage groups, which had a total length of 1683.8 cM with an average interval of 7.3 cM. The genome coverage of the integrated map was approximately 86.3%. The genetic linkage map would form the foundation for further studies on the quantitative trait loci(QTL), as well as accelerating the breeding process of this species.     

Levels of Metabolic Enzymes and Nitrogenous Compounds in the Swimming Crab Portunus trituberculatus Exposed to Elevated Ambient Ammonia-N

This study investigated the effects of ammonia-N(control and 1 and 5 mg L~(-1)) on the changes of metabolic enzymes activities and nitrogenous compounds levels in the gills, hepatopancreas, muscles, and hemolymph of the swimming crab Portunus trituberculatus. Results showed that the levels of hemolymph ammonia increased significantly at 6 h and then decreased to control level at 12 h. The activities of key enzymes involved in biosynthesis pathways of glutamine(Gln) and urea, such as glutamate dehydrogenase(GDH), glutamine synthetase(GS), and arginase(ARG), increased remarkably in different tissues of P. trituberculatus. Gln level was the highest in muscles, while urea content was the largest in hepatopancreas. The m RNA expression level of xanthine dehydrogenase(XDH) was downregulated sharply in gills but upregulated in the hepatopancreas and muscles. A small amount of uric acid was detected in the hemolymph. This result suggests that Gln in muscles may be used as an intermediary nitrogen repository, while urea may be primarily synthesized in the hepatopancreas and then transferred to other tissues via the hemolymph. Uric acid could be synthesized by the anabolism of purine nucleotides, in additon to the inhibitory activities of gills. In addition, the levels of Gln, urea and uric acid exhibited dose-and time-dependent effects with ammonia content. Therefore, P. trituberculatus could possibly convert ammonia into Gln, urea, or uric acid to detoxify ammonia during high ambient ammonia-N stress.     

Cd~(2+)对青蛤(Cyclina sinensis)的毒性及蓄积过程研究

利用水生动物急性毒性实验方法,在6个浓度下Cd2+对青蛤的急性毒性进行测定,经回归分析后,得到Cd2+对青蛤96h半致死浓度为20.09mg/L,安全浓度为0.201mg/L。分别对半致死浓度和安全浓度下Cd2+在青蛤体内不同组织的蓄积情况进行了分析。结果表明,在半致死浓度条件下,168h青蛤各组织Cd2+的含量依次为肌肉内脏团鳃;而在安全浓度下,其结果为内脏团鳃肌肉,内脏团对于Cd2+富集能力远高于肌肉。讨论了青蛤的养殖环境及产品检验等问题。     

鳗弧菌(Vibrio anguillarum)侵染对青蛤(Cyclina sinensis)磷酸酶活性的影响

对500个成体青蛤分别用鳗弧菌和生理盐水注射,在感染后3h、6h、12h、24h和36h分别取不同处理组的肝胰脏、鳃和闭壳肌组织,测定上述样品的碱性磷酸酶(ALP)及酸性磷酸酶(ACP)活性,分析鳗弧菌对青蛤体内免疫相关酶活性的影响。结果表明,鳗弧菌感染组的ALP与ACP活性从高到低依次为肝胰脏鳃闭壳肌,其中青蛤肝胰脏和鳃组织中ALP和ACP活性均有显著升高的趋势,并且在12h时达到最高,与对照组差异显著(P0.05),随后呈现下降趋势。而青蛤闭壳肌组织中侵染组ALP和ACP活性与对照组之间没有显著差异(P0.05)。鳗弧菌对青蛤的磷酸酶活性影响较大,对其免疫防御系统有明显的刺激作用。     

青岛海洋趋磁球菌QH-3 的鞭毛特征

趋磁细菌(magnetotactic bacteria,MTB)是一类能够沿着磁力线运动的特殊细菌。MTB在胞内磁小体的导向下借助自身鞭毛进行趋磁趋氧运动,以到达适宜的生境。本研究在青岛潮间带沉积物中发现大量的趋磁球菌,通过光镜与透射电镜对其运动方式与显微结构进行观察。结果显示优势趋磁细菌为卵球形,大小为2.5μm×2.1μm,宽长比为0.85,将其命名为QH-3。QH-3为趋北型(North-seeking)MTB,运动速度88μm/s,细胞内两侧各有一条由棱柱形颗粒组成的磁小体链。QH-3在菌体同一侧有两个由直径62 nm±9 nm的鞘包裹7根鞭毛组成的鞭毛束。这种特殊的鞭毛结构与已报道的致病菌的不同,而与法国地中海趋磁球菌MO-1的相似。     

环境样品中甲基汞的检测方法综述

综述了常用的环境样品中甲基汞的检测方法和检测步骤,认为在实际的甲基汞检测过程中,选择或评价所选用检测方法的原则通常是,在确保检测限的基础上,首选考虑检测结果的精确度如何,而快捷方便、经济实惠的检测技术也是现实运用中的实际需要。作为定量检测,GC分别与AAS、AES、AFS的联用是目前较常用的技术,而与ICP-MS或ID-ICPMS的联用,在今后的实际检测中将得到更广泛的运用。生物传感器检测到的汞一般是生物可利用的汞,降低其检测限并将其作为对污染水体与沉积物检测的重要方法,也是今后研究的重点。总之,在人类对环境安全和身体健康日益重视的今天,发展高灵敏度、高准确度、快速方便和低成本的检测技术是未来甲基汞检测的重要发展方向。     

基于SPI接口和FIFO缓冲器的大容量高速实时数据存储方案

以海洋湍流观测样机中的数据存储接口电路为例,介绍一种基于C8051单片机的SPI(串行外设接口)、FI-FO(先入先出)缓冲器IDT72v01和闪存M25P64的大容量高速实时数据存储方案。该方案具有简易实用、运行稳定、低功耗、易扩展的特点,已成功应用于已开发的湍流监测仪器样机。     

海岸系统演变长周期数值模拟研究进展

在人类活动日益频繁及海平面上升等自然环境变化的背景下,研究和预测海岸系统地貌演变正变得越来越重要。长周期数值模型是海岸系统演变研究的一种重要手段,近年来得到了迅速发展和广泛应用。介绍了海岸系统演变的长周期数值模型的计算原理、信息约减和地貌加速等关键技术、时空尺度及其验证方法。详细阐述了不同类型长周期模型在海岸系统演变的过程与机制和长期演变的数值模拟及预测人类活动和自然变化引起的海岸系统长期演变等方面的应用与最新进展,并对长周期模型的发展进行了讨论。