Prevalence and diversity of norovirus genogroups I and II in Hong Kong marine waters and detection by real-time PCR

Marine waters from six sites around Hong Kong with varying levels of sewage pollution were examined for noroviruses (NoVs) by PCR cloning and sequencing of a highly-variable N-terminal region of the VP1 capsid gene, at the ORF1-ORF2 junction of NoV. Phylogenetic analysis of genogroups GI- and GII-specific PCR clones obtained from different marine sites indicated that human NoV GI.1 and GII.4 strains are the most prevalent genotypes circulating in Hong Kong waters. GI- and GII-specific TaqMan-based real-time PCR assays targeting the ORF1-ORF2 junction of NoVs were used to quantify NoV particles in marine water samples in parallel with total Escherichia coli counts which were enumerated on TBX medium. No correlation of any significance between NoV and E. coli counts was observed which highlighted the inadequacy in using E. coli as a fecal indicator to predict the level of NoVs in marine waters to protect public health.     

Distinguishing streamflow trends caused by changes in climate,forest cover,and permafrost in a large watershed in northeastern China

Understanding how rivers respond to changes in land cover, climate, and subsurface conditions is critical for sustainably managing water resources and ecosystems. In this study, long‐term hydrologic, climate, and satellite data (1973–2012) from the Upper Tahe River watershed (2359 km²) in the Da Hinggan Mountains of northeast China were analysed to quantify the relative hydrologic effects of climate variability (system input) and the combined influences of forest cover change and permafrost thaw (system characteristics) on average annual streamflow (system response) using 2 methods: the sensitivity‐based method and the Kendall–Theil robust line method. The study period was subdivided into a forest harvesting period (1973–1987), a forest stability period (1988–2001), and a forest recovery period (2002–2012). The results indicated that the combined effects of forest harvesting and permafrost thaw on streamflow (+ 47.0 mm) from the forest harvesting period to the forest stability period was approximately twice as large as the effect associated with climate variability (+20.2 mm). Similarly, from the forest stability period to the forest recovery period, the decrease in average annual streamflow attributed to the combined effects of forest recovery and permafrost thaw (?38.0 mm) was much greater than the decrease due to climate variability (?22.2 mm). A simple method was used to separate the distinct impacts of forest cover change and permafrost thaw, but distinguishing these influences is difficult due to changes in surface and subsurface hydrologic connectivity associated with permafrost thaw. The results highlight the need to consider multiple streamflow drivers in future watershed and aquatic ecosystem management. Due to the ecological and hydrological susceptibility to disturbances in the Da Hinggan Mountains, forest harvesting will likely negatively impact ecohydrological processes in this region, and the effects of forest species transition in the forest recovery process should be further investigated.     

Effects of bottom sediments on the survival of Enterobacter aerogenes in seawater

The survival of the coliform bacterium Enterobacter aerogenes in seawater was greatly affected by the underlying bottom sediments. The presence of sediments which contained higher concentrations of organic nutrients than the overlaying seawater prolonged the survival and even enabled growth of the bacterial cells. Results obtained from chemical analysis indicated that sediments of fine-grained, silt-clay type contained highest amounts of organic nutrients as compared with sediments with larger particle sizes such as silt and sand grains. Thus, nutrient contents of the sediments were found to be inversely proportional to sediment particle size. The degree of prolonged survival of E. aerogenes was also found to be inversely proportional to the size of the sediment particles presented. Release of the bound nutrients which became available to cells of E. aerogenes from sediments by action of waves and human activities can explain, at least in part, why high counts of coliform bacteria are obtained in coastal and beach waters.     

塔里木盆地西北缘海相白垩系-古近系界线沉积物环境指标研究

发生于中生代-新生代之间的事件是地质历史上一次重大灾变和异常事件,因此白垩系-古近系之间的界线(K/T界线)就成为全球最重要的地质界线之一.通过对塔里木盆地西北缘乌恰地区库孜贡苏河剖面海相上白垩统-古新统地层的岩性和沉积环境变化以及地球化学特征研究,重点利用沉积物色度、有机碳含量、碳酸钙和碳、氧稳定同位素的明显异常变化...     

基于ARP协议的网络主机地址的识别技术研究

从移动IPv6工作原理入手，IP地址管理器是针对越来越严重的IP地址盗用现象而设计的一套有效的监控和管理IP地址的方案。分析与研究IP地址管理器中的信息收集模块后，以Winpcap为开发工具，利用ARP，附等协议完成对网络中活动主机的扫描以及信息（主机名、MAC、操作系统）的收集和识别。模块实现了对网络的自动实时监控，有效地提高了管理效率。     

PM2.5污染对中国人口死亡率的影响——基于346个城市面板数据的实证分析

中国生态文明建设和“健康中国”战略强调切实治理影响人口健康的环境问题,建设健康人居环境。论文基于2000年和2010年中国人口普查资料以及2005年和2015年各省级行政单元1%人口抽样调查等数据资料,论文使用探索性空间分析方法刻画中国城市人口死亡率的时空变化特征,并采用空间回归方法,揭示城市PM_2.5的平均浓度对人口死亡率的影响及其空间溢出效应,以及社会经济因素对PM_2.5—人口死亡率关联的调节效应。结果表明：① 中国城市人口死亡率的空间分布特征呈现明显的异质性,高死亡率地区早期集聚分布于西南地区,2005年后在西南地区、华北地区、华东地区和华中地区呈现逐渐集聚分布态势。低死亡率地区长期集中分布于西北地区、东北地区、长三角地区、珠三角地区和京津两市。② 人口死亡率的分布存在空间关联性,高—高类型地区早期集中分布于西南地区,后期向东扩展;低—低类型地区主要分布于北疆、内蒙古西部和广东省及其周边地区。③ 城市PM_2.5浓度对人口死亡率具有显著的正向影响,并且对邻近地区的人口死亡率具有显著的空间溢出效应。④ 中国城市PM_2.5浓度对人口死亡率的影响存在学历差异和城乡差异,地区高学历人群集聚可降低PM_2.5的健康风险,城镇化发展进程缓慢则会加重PM_2.5的健康风险。研究旨在为防范空气污染暴露导致的健康风险、建设健康人居环境提供科学依据。     

Extrusion movements of a tunnel head reinforced by finite length bolts—a closed‐form solution using homogenization approach

An analytical model on the general behaviour of a tunnel head, reinforced by finite length bolts is proposed. This model is based on the homogenization method and spherical symmetry assumption. Despite its simplicity, and in consequence its limits of validity, it does allow a quick estimation of the key design parameters: frontal displacement, extension of decompressed zone, ground stresses and bolt tension, and constitutes thereby a very useful and handy tool for design engineers. In particular, the influence of the reinforcement length, as well as other important design parameters, are studied by the proposed model. The charts resulting from the parametric studies are directly applicable. Otherwise, the comparison to a 3D numerical model is also presented in this paper. The first results provide the validation of the analytical solution, at least in terms of average extrusion movements. Copyright © 2000 John Wiley & Sons, Ltd.     

»Late Jurassic« petroleum geology of the Dutch Central North Sea Graben

The Callovian to Ryazanian (late Middle Jurassic to earliest Cretaceous; here referred to as »Late Jurassic«) sediments of the Dutch Central North Sea Graben, comprising the Central Graben and Scruff Groups, form several potential reservoirs. Source rocks are present within both these groups and the Carboniferous and Late Triassic-Early Jurassic.The structural development of the area is illustrated by a number of cross-sections. A structural subdivision has been made into platform, intermediate platform, outer graben, and inner graben. The complex history of rifting, wrench-faulting, halokinesis, and structural inversion strongly influenced the palaeogeography and ensuing sandstone developments. A few selected burial-history plots are presented as examples of the relationship between the structural subdivision and the hydrocarbon potential of the area.

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Zusammenfassung Die Sedimente des Callov bis zum Ryazan (oberer Mitteljura bis unterste Kreide, hier als »Oberjura« bezeichnet) des zentralen niederländischen Nordsee-Grabens, der den Zentralgraben und die Scruff-Gruppe umfaßt, stellen mehrere potentielle Erdölspeichergesteine dar. In jeder dieser beiden Gruppen sowie im Karbon und von der Obertrias bis zur Unterkreide existieren Speichergesteine.Die strukturelle Entwicklung des Gebietes wird anhand mehrere Profile veranschaulicht. Außerdem schien eine Untergliederuntg in Plattform, Zwischenplattform, äußerer sowie innerer Graben sinnvoll. Die komplexe Entwicklungsgeschichte, die durch Riftingphasen, Wrench-Tektonik, Halokinese und Strukturinversion bestimmt wurde, hat die Paläogeographie und die resultierende Sandsteinbildung intensiv beeinflußt. Eine Anzahl ausgewählter Darstellungen der Geschichte der Sedimentüberlagerung dienen als Beispiele für den Zusammenhang zwischen den strukturell bestimmten Untergruppen und dem Kohlenwasserstoffpotential des Untersuchungsgebietes.

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Résumé Dans le secteur néerlandais du Graben Central de la Mer du Nord, plusieurs réservoirs potentiels sont constitués par les sédiments calloviens à ryazaniens (ensemble allant du sommet du Jurassique moyen au Crétacé inférieur et désigné ici par «Late Jurassic»). Ces sédiments comprennent le groupe du Graben central et le groupe de Scruff. Des rochesmères existent dans chacun de ces deux groupes ainsi que dans le Carbonifère et dans la série Trias terminal-Eojurassique.La constitution structurale de cette région est illustrée par une série de coupes. On distingue, au point de vue structural: une plate-forme, une plate-forme intermédiaire, un graben externe et un graben interne. L'histoire géologique complexe, avec ses processus de rifting, de décrochement, d'halokinèse et d'inversion structurale, s'est répercutèe de manière significative sur la paléogéographie et, par voie de conséquence, sur l'extension des faciès arénacés. Quelques reconstitutions de l'histoire sédimentaire sont présentées à titre d'exemple de manière à illustrer la relation entre les unités structurales et le potentiel en hydrocarbure de la région.

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Cordierite breakdown under high-pressure hydrous conditions

Green and Vernon (1974) have experimentally determined a divariant field of cordierite-orthoamphibole-kyanite-quartz with a slope of 12±4 bars per °C occurring between 8.0 and 10.4 kbar at 750° C and between 9.5 and 11.3 kbar at 850° C. However, the writers' thermodynamic calculation of the divariant band indicates a more limited range of 0.65 and 0.7 kbar at 750° C and 850° C respectively, with an estimated error of ±0.2 kbar.