东山湾贝类养殖容量的估算

以初级生产力为基础，应用营养动态模式和沿岸海域能流分析模型，估算东山湾贝类年生产量分别为269331t和295767t，平均282549t。扣除潮间带、潮下带和吊养区浮筏、随着基及延绳上附着的非养殖滤食性附着动物自然现存量35760t，贝类可养殖量为246789t。应用统计分析和逻辑斯谛种群增长模型估计贝类可养面积为11839hm^2，其中缢蛏635hm^2，石蛎1160hm^2,吊蛎1525hm^2，翡翠贻贝633hm^2，菲律宾蛤仔1154hm^2，扇贝698hm^2，江瑶248hm^2，泥蚶601hm^2，巴非蛤4151hm^2，凸壳肌蛤1034hm^2。1998年实际养殖面积和养殖产量分别为10581hm^2和220564t。尚有1258hm^2,26225t的扩大开发潜力。文中还讨论了合理布局和优化养殖结构问题。     

麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。     

莱州湾温带风暴潮预报研究

本文依据莱州湾羊角沟、夏营两站建国以来的风暴增水资料，对莱州湾建国后发生的风暴潮进行了统计分析，并探讨了温带风暴潮产生的物理机制，此外还对莱州湾温带风暴增水以及诱发增水的天气形势进行了分析分类。在此基础上建立了莱州湾温带风暴潮统计预报方法，并在作业预报中对模型进行了检验，取得较为理想的效果。     

珊瑚礁岩心声速垂向跃变及其指相意义

本文在南沙群岛永署礁南永1井珊瑚礁岩心声速测量的基础上，研究了珊瑚礁岩心声速的垂向跃变特征和相应地层沉积相变及地层侵蚀间断面的关系，分析了珊瑚礁岩心相变造成其声速跃变的原因，阐明了海平面升降形式珊瑚礁地层侵蚀间断面和产生相变的具体过程, 明了珊瑚礁岩心声速垂向跃变与相应地层古气候和海平面变化的关系，该项研究在岛礁工程建设和礁灰岩区石油勘探声学测井以及西太平洋边缘海古海洋学研究学方面具有重要的实用价值和理论意义。     

花尾胡椒鲷养殖群体遗传多样性的等位酶研究

应用聚丙烯酰胺凝胶电泳（PAGE）技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明：所检测的16种同工酶的24个基因座位中，在XDH和GCDH基因座位上发现多态性，其平均多态位点比例为8．33％，基因座位有效等位基因的平均数Ne为1.083，平均杂合度的观测值Ho为0．00556，预期值He为0．00537,Hardy-Weinberg遗传偏离指数（D)为0．035。与其他鱼类相比较说明了其遗传多样性处于较低水平，文中对此进行了探讨并提出相应的种质资源管理保护措施。     

Preliminary study on different nutrient pools supplies for the phytoplankton growth in the Jiaozhou Bay in China in the fall of 2004

The source and significance of two nutrients, nitrogen and phosphorous, were investigated by a modified dilution method performed on seawater samples from the Jiaozhou Bay, in autumn 2004. This modified dilution method accounted for the phytoplankton growth rate, microzooplankton grazing mortality rate, the internal and external nutrient pools, as well as nutrient supplied through remineralization by microzooplankton. The results indicated that the phytoplankton net growth rate increased in turn from inside the bay, to outside the bay, to in the Xiaogang Harbor. The phytoplankton maximum growth rates and microzooplankton grazing mortality rates were 1.14 and 0.92 d-1 outside the bay, 0.42 and 0.32 d-1 inside the bay and 0.98 and 0.62 d-1 in the harbor respectively. Outside the bay, the remineralized nitrogen (Kr=24.49) had heavy influence on the growth of the phytoplankton. Inside the bay, the remineralized phosphorus(Kr=3.49) strongly affected the phytoplankton growth. In the harbor, the remineralized phosphorus (Kr=3.73) was in larger demand by phytoplankton growth. The results demonstrated that the different nutrients pools supplied for phytoplankton growth were greatly in accordance with the phytoplankton community structure, microzooplankton grazing mortality rates and environmental conditions. It is revealed that nutrient remineralization is much more important for the phytoplankton growth in the Jiaozhou Bay than previously believed.     

杂交鲆(牙鲆♀×夏鲆♂)胚胎发育的初步观察

通过人工调控促使亲本牙鲆(Paralichthys olivaceus)♀×夏鲆(Paralichthys dentatus)♂的性腺发育,人工干法授精获得杂交受精卵。杂交受精卵在温度为16.5~17.5℃,光照为100~400 lx,微充气条件下孵化。对杂交鲆胚胎发育全过程进行连续观察并与牙鲆及夏鲆的胚胎发育进行比较。结果表明:杂交鲆卵裂方式与其他大部分硬骨鱼类一样,属于盘状卵裂。受精后经过2 h 30 min进入2细胞期,7 h 45 min进入囊胚期,15 h 10 min进入原肠胚阶段,25 h 30 min进入神经胚期,49 h左右进入尾芽期。授精后63 h左右发现初孵仔鱼,68h左右50%的仔鱼孵出。在杂交鲆的胚胎发育中,主要有2个发育特征与母本类似而与父本不同:视囊的出现要早于体节的分化;孵化前,仔鱼在卵膜内环绕卵黄囊不到1周。但是在其孵化时间上却与父本相似。     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。     

Seasonality and interannual variability of freshwater inflow to a large oligohaline estuary in the Northern Gulf of Mexico

Quantity, timing, duration, and fluctuation of freshwater inflow are important factors affecting the development and health of aquatic and adjacent wetland ecosystems in coastal estuaries. This study assessed six decades of freshwater inflow from the Amite River, Tickfaw River, and Tangipahoa River watersheds to Lake Pontchartrain, a large oligohaline estuary in the Northern Gulf of Mexico, whose flood waters caused recent damage to the city of New Orleans in the aftermath of Hurricane Katrina. By utilizing the long-term (1940–2002) river discharge and climatic data from the three major tributary watersheds, monthly and annual freshwater inflows have been quantified and their spatial and temporal variations have been analyzed. On average, the three rivers discharged (±standard error) 0.27 ± 0.04 km³ freshwater monthly and 3.29 ± 0.15 km³ freshwater annually into the lake estuarine system, with the highest inflow from the Amite River (0.16 ± 0.03 m³ mon⁻¹, and 1.91 ± 0.09 km³ yr⁻¹) and the lowest inflow from the Tickfaw River (0.03 ± 0.00 km³ mon⁻¹, and 0.34 ± 0.02 km³ yr⁻¹). A distinct seasonality was evident with over 69% of the total annual inflow occurring during December and May (wet months) and with a low flow period from August to November (dry months). The monthly inflow during the wet months was positively correlated with the monthly precipitation (r² = 0.64), while the monthly inflow during the dry months was subject to evapotranspiration. Furthermore, the study found a 20-year low flow period from 1954–1973 (2.76 ± 0.24 km³ yr⁻¹) and a 24-year high flow period from 1975–1998 (3.84 ± 0.24 km³ yr⁻¹), coinciding with both the climate variation and population growth in the watersheds.     

0608号超强台风"桑美"的主要特点和风暴潮影响特征分析

0608号超强台风"桑美"正面袭击了温州,给温州造成了严重的人员伤亡和经济损失。本文利用相关气象和海洋资料分析了台风"桑美"的主要特点及其风暴潮影响特征。