The assessment of vitellogenin as a biomarker of exposure to estrogenic compounds in two Australian perciformes

Vitellogenin (Vtg) is a yolk protein precursor that has been identified as a sensitive biomarker for exposure to estrogenic compounds. We evaluated specific monoclonal and polyclonal antibodies for reactivity with plasma Vtg from two Australian Perciformes, the tropical barramundi (Lates calcarifer) and the temperate black bream (Acanthopagrus butcheri). Blood plasma from 17beta-estradiol exposed (E2) male barramundi (20 mg kg(-1)) and male black bream (2.5-5.0 mg kg(-1)) were sent to Biosense Laboratories (Norway) for cross-reactivity testing using their extensive anti-Vtg antibody selection. Indirect ELISA results determined barramundi plasma displayed the highest binding affinities to ND-3G2 (monoclonal-Mab) and PO-1 (polyclonal-Pab). Black bream was most cross-reactive with ND-1C8 (Mab) and PO-2 (Pab). Next, plasma was assessed for Vtg induction in E2-dosed (5 mg kg(-1)), hatchery-reared barramundi and black bream versus a non-injected control group. Vtg production was assessed by Western blot and indirect ELISA using ND-3G2 and ND-1C8 Mabs, respectively. A prominent band was identified in the range of 100-200 kDa for all female black bream and for all E2-treated barramundi and black bream males, which was confirmed as Vtg by Western blot. Indirect ELISA results for barramundi demonstrated highly significant differences in E2-dosed fish as compared to control fish (Student t, P<0.001). E2 male black bream were significantly different than control males (Student t, P<0.001) and control and E2 females displayed highly significant differences (Student t, P<0.001). These results indicate that exposure to 17beta-estradiol induces significant Vtg production in males of the two Australian Perciformes, with potential use as a biomarker for exposure to estrogenic compounds.     

Herbicide contamination and the potential impact to seagrass meadows in Hervey Bay, Queensland, Australia

Low concentrations of herbicides (up to 70 ng l(-1)), chiefly diuron (up to 50 ng l(-1)) were detected in surface waters associated with inter-tidal seagrass meadows of Zostera muelleri in Hervey Bay, south-east Queensland, Australia. Diuron and atrazine (up to 1.1 ng g(-1) dry weight of sediment) were detected in the sediments of these seagrass meadows. Concentration of the herbicides diuron, simazine and atrazine increased in surface waters associated with seagrass meadows during moderate river flow events indicating herbicides were washed from the catchment to the marine environment. Maximum herbicide concentration (sum of eight herbicides) in the Mary River during a moderate river flow event was 4260 ng l(-1). No photosynthetic stress was detected in seagrass in this study during low river flow. However, with moderate river flow events, nearshore seagrasses are at risk of being exposed to concentrations of herbicides that are known to inhibit photosynthesis.     

Efficacy of PIT tags and an autonomous antenna system to study the juvenile life stage of an estuarine-dependent fish

Many marine fishes use spatially distinct habitats as juveniles and adults. Determining which juvenile habitats are most important to sustaining adult populations (i.e., which habitats are nurseries) has proven difficult, in part due to challenges in estimating survival of juveniles in putative nursery habitats. Recent technological advances have made largescale tagging efforts a viable approach to estimating survival of juvenile fishes by increasing recapture rates and enabling the use of individual-identification tags. These techniques, using Passive Integrated transponder (PIT) tags and autonomous antenna detection systems (antenna), have been successfully applied in freshwater environments. This paper reports the adaptation of these techniques to estuarine mangrove creeks (salinity: 2–28‰) for research of the juvenile life stage on an estuarine-dependent marine fish,Centropomus undecimalis. Retention rate of PIT tags in juveniles >120 mm standard length was 100%, with no mortality. The antenna detection field covered 48% of the creek water column, and the antenna was experimentally determined to detect approximately 67% of tagged fish that swam through. Overall recapture rate of tagged fish by the antenna was >40%. This recapture rate is higher than the sparse data typical of traditional tag-recapture studies. A time-dependent Jolly-Seber model was fit to the data, providing estimates of capture probability (0.8) and weekly apparent survival (0.41) that will be invaluable in comparing juvenile habitats of different quality (e.g., natural versus anthropogenically degraded). This research demonstrates the viability of this approach to fish research in estuarine habitats.     

Solubility and solution mechanisms of C-O-H volatiles in silicate melt with variable redox conditions and melt composition at upper mantle temperatures and pressures

Solubility and solution mechanisms in silicate melts of oxidized and reduced C-bearing species in the C-O-H system have been determined experimentally at 1.5 GPa and 1400 °C with mass spectrometric, NMR, and Raman spectroscopic methods. The hydrogen fugacity, f_H2, was controlled in the range between that of the iron-wüstite-H₂O (IW) and the magnetite-hematite-H₂O (MH) buffers. The melt polymerization varied between those typical of tholeiitic and andesitic melts.The solubility of oxidized (on the order of 1-2 wt% as C) and reduced carbon (on the order of 0.15-0.35 wt% as C) is positively correlated with the NBO/Si (nonbridging oxygen per silicon) of the melt. At given NBO/Si-value, the solubility of oxidized carbon is 2-4 times greater than under reducing conditions. Oxidized carbon dioxide is dissolved as complexes, whereas the dominant reduced species in melts are CH₃-groups forming bonds with Si⁴⁺ together with molecular CH₄. Formation of complexes results in silicate melt polymerization (decreasing NBO/Si), whereas solution of reduced carbon results in depolymerization of melts (increasing NBO/Si).Redox melting in the Earth’s interior has been explained with the aid of the different solution mechanisms of oxidized and reduced carbon in silicate melts. Further, effects of oxidized and reduced carbon on melt viscosity and on element partitioning between melts and minerals have been evaluated from relationships between melt polymerization and dissolved carbon combined with existing experimental data that link melt properties and melt polymerization. With total carbon contents in the melts on the order of several mol%, mineral/melt element partition coefficients and melt viscosity can change by several tens to several hundred percent with variable redox conditions in the range of the Earth’s deep crust and upper mantle.     

Ferruginous thermal spring complexes, northwest Tasmania: evidence that far-field stresses acting on a fracture mesh can open and maintain vertical flow in carbonate terrains

Far-field stress changes in the southern Australian plate since 5 Ma have produced significant areas of uplift and seismicity. In northwest Tasmania, there is evidence that this stress reorientation to maximum horizontal NW?CSE stress has influenced meteoric-derived thermal (15?C20°C) discharge patterns of confined karstic aquifers, by placing pre-existing NW-trending faults/fractures into a dilated state or a critically stressed state. Previous studies have shown that spring discharge has operated continuously for at least 65,000 years, and has transported large volumes of solutes to the surface to be deposited as mounds of calcite-goethite-silica up to 7 m high. The thermal spring chemistry at one site, Mella, is consistent with descent to at least 1.2?C1.5 km, although the hinterland within 50 km is less than 500 m elevation. Thermal spring chemistry is consistent with most of the deep water?Crock interaction occurring in low-strontium Smithton Dolomite. While some of this water is discharged at springs, some instead intersects shallow zones of NE-fracture-controlled rock (2?×?4 km in area) with karstic permeability where, although confined and subject to a NE-directed hydraulic gradient, it circulates and cools to ambient temperature, with only minor mixing with other groundwaters.     

Sources of Terrestrial Organic Carbon in the Mississippi Plume Region: Evidence for the Importance of Coastal Marsh Inputs

High sedimentation rates along river-dominated margins make these systems important repositories for organic carbon derived from both allochthonous and autochthonous sources. Using elemental carbon/nitrogen ratios, molecular biomarker (lignin phenol), and stable carbon isotopic (bulk and compound-specific) analyses, this study examined the sources of organic carbon to the Louisiana shelf within one of the primary dispersive pathways of the Mississippi River. Surface sediment samples were collected from stations across the inner, mid, and outer Louisiana shelf, within the Mississippi River plume region, during two cruises in the spring and fall of 2000. Lignin biomarker data showed spatial patterns in terrestrial source plant materials within the river plume, such that sediments near the mouth of the Mississippi River were comparatively less degraded and richer in C₄ plant carbon than those found at mid-depth regions of the shelf. A molecular and stable isotope-based mixing model defining riverine, marsh, and marine organic carbon suggested that the highest organic carbon inputs to the shelf in spring were from marine sources (55?C61% marine organic carbon), while riverine organic carbon was the highest (63%) in fall, likely due to lower inputs of marine organic carbon at this time compared with the spring season. This model also indicated that marsh inputs, ranging from 19 to 34% and 3?C15% of the organic carbon in spring and fall, respectively, were significantly more important sources of organic carbon on the inner Louisiana shelf than previously suggested. Finally, we propose that the decomposition of terrestrial-derived organic carbon (from the river and local wetlands sources) in mobile muds may serve as a largely unexplored additional source of oxygen-consuming organic carbon in hypoxic bottom waters of the Louisiana shelf.     

Future projections of temperature-related climate change impacts on the railway network of Great Britain

Great Britain’s main line railway network is known to experience various temperature-related impacts, e.g. track buckling and overhead power line sag at high ambient temperatures. Climate change could alter the frequency of occurrence of these impacts. We have therefore investigated the climate change impact on various temperature-related issues, identified during workshops with rail industry specialists, using a perturbed physics ensemble (PPE) of the Met Office’s regional climate model (RCM), HadRM3. We have developed novel approaches to combine RCM data with railway industry knowledge, typically by identifying key meteorological thresholds of interest and analysing exceedance of these out to the 2040s. We performed a statistical analysis of the projected changes for each issue, via bootstrapping of the unperturbed PPE member. Although neither the PPE nor the bootstrapping analysis samples the full range of uncertainty in the projections, they nonetheless provide complementary perspectives on the suitability of the projections for use in decision-making. Our main findings include projected increases in the summertime occurrence of temperature conditions associated with (i) track buckling, (ii) overhead power line sag, (iii) exposure of outdoor workers to heat stress, and (iv) heat-related delays to track maintenance; and (v) projected decreases in the wintertime occurrence of temperatures conditions associated with freight train failure owing to brake problems. For (i), the statistical significance varied with track condition and location; for (ii) and (iii), with location; and for (iv) and (v), projected changes were significant across Great Britain. As well as assessing the changes in climate-related hazard, information about the vulnerability of the network to past temperature-related incidents has been summarised. Combining the hazard and vulnerability elements will eventually support a climate risk assessment for the industry.     

Nonaxisymmetric instabilities in self-gravitating disks. II. Linear and quasi-linear analyses

We studied global nonaxisymmetric hydrodynamic instabilities in an extensive collection of hot, self-gravitating polytropic disk systems, systems that covered a wide expanse of the parameter space relevant to protostellar and protoplanetary systems. We examined equilibrium disk models varying three parameters: the ratio of the inner to outer equatorial radii, the ratio of star mass to disk mass, and the rotation law exponent q. We took the polytropic index n=1.5 and examined the exponents q=1.5 and 2, and the transitional one q=1.75. For each of these sets of parameters, we examined models with inner to outer radius ratios from 0.1 to 0.75, and star mass to disk mass ratios from 0 to 10³. We numerically calculated the growth rates and oscillation frequencies of low-order nonaxisymmetric disk modes, modes with azimuthal dependence ∝e ^im? . Low-m modes are found to dominate with the character and strength of instability strongly dependent on disk self-gravity. Representatives of each mode type are examined in detail, and torques and mass transport rates are calculated.     

Role of water in the formation of the Late Cretaceous Wetumpka impact structure,inner Gulf Coastal Plain of Alabama,USA

Abstract— The effect of shallow marine water (?30–100 m deep) in the late excavation and early modification stages of a marine‐target crater 5 km in diameter, as exemplified by the Late Cretaceous Wetumpka impact structure in Alabama, USA, is manifest in the early collapse of a weak part of the rim. Excavation flow and connate marine water are interpreted to be factors in this collapse. This partial rim collapse catastrophically emplaced an upper‐structure‐filling unit of broken and redistributed sedimentary target formations, which presently mantles the deeper fallback breccia deposits within the structure. Furthermore, rim collapse flow facilitated the formation of a structurally modified, extrastructure terrain, which is located outside and adjacent to the collapsed rim segment. This extrastructure terrain appears to be the product of extensive slumping of poorly consolidated target sedimentary formations.     

A two-level perturbation method for connecting unstable periodic orbits with low fuel cost and short time of flight: application to a lunar observation mission

The proposed method connects two unstable periodic orbits by employing trajectories of their associated invariant manifolds that are perturbed in two levels. A first level of velocity perturbations is applied on the trajectories of the discretized manifolds at the points where they approach the nominal unstable periodic orbit in order to accelerate them. A second level of structured velocity perturbations is applied to trajectories that have already been subjected to first level perturbations in order to approximately meet the necessary conditions for a low \(\varDelta \text {V}\) transfer. Due to this two-level perturbation approach, the number of the trajectories obtained is significantly larger compared with approaches that employ traditional invariant manifolds. For this reason, the problem of connecting two unstable periodic orbits through perturbed trajectories of their manifolds is transformed into an equivalent discrete optimization problem that is solved with a very low computational complexity algorithm that is proposed in this paper. Finally, the method is applied to a lunar observation mission of practical interest and is found to perform considerably better in terms of \(\varDelta \text {V}\) cost and time of flight when compared with previous techniques applied to the same project.