A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.     

Morphological and molecular evidence supports the first occurrence of two fishes,Siganus sutor(Valenciennes, 1835)and Seriolina nigrofasciata(Rüppell, 1829)(Actinopterygii:Perciformes), from marine waters of Odisha coast,Bay of Bengal,India

Marine ecosystems provide a wide variety of diverse habitats that frequently promote migration and ecological adaptation. The extent to which the geographic distribution of marine organism has reshaped by human activities remains underappreciated. The limitations intrinsic to morphology-based identification systems have engendered an urgent need for reliable genetic methods that enable the unequivocal recognition of fish species,particularly those that are prone to overexploitation and/or market substitution. In the present study, however, an attempt has been taken to identify two locally adapted fish species, Siganus sutor(Valenciennes, 1835) and Seriolina nigrofasciata(Rüppell, 1829) of order Perciformes, which happens to be the first record in Odisha coast,Bay of Bengal. The diagnostic characteristics of Siganus sutor are: dorsal fin XIII-10, anal fin VII-9, pectoral fin 15,pelvic fin II-3, while that of Seriolina nigrofasciata dorsal fin VI-I-35, anal fin I-17, pectoral fin 16, pelvic fin 5. All COI barcodes generated in this study were matched with reference sequences of expected species, according to morphological identification. Bayesian and likelihood phylogenetic trees were drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at the species level. The phylogeographic studies based on haplotype network and migration rates suggest that both the species were not panmitic and the high-frequency population distribution indicates successful migration. The result of this study provides an important validation of the use of DNA barcode sequences for monitoring species diversity and changes within a complex marine ecosystem.     

浙江沿岸第四纪沉积物中重金属形态的初析

本文报导了1983年8月于浙江沿岸采集的两个第四纪柱状样品中重金属的形态和分布,并采用连续提取法,将沉积物中重金属分成六种形态:即水可溶态;氯化镁可交换态;碳酸盐结合态;铁锰水合氧化物结合态;有机质结合态;残余态。然后分别用阳极溶出伏安法和原子吸收分光光度法测定。     

一九三五年八月德国气象学会会议纪要

德国气象学会,每两年举行会议一次,其先已於一九三一年及一九三三年分别在维也纳及汉堡举行。今年八月二十五日至二十七日为第十九次会期,假但泽高等工业学校开会,与会者除德人而外颇多外人。其演讲多属天气与大气之各种现象,兹择要略纪如下。会议开幕时,由主席枢密顾问明兴Schmauss教授演讲“气象学之术语”。略谓:吾人皆知气象界须有统一之国际符号,以为发表工具,使逐日印行之天气图与各处之年报,均有同一之记载。教授继谓气象学上基本术语,应有对外对内之分,前者吾人仅需知其国际简单意义,而後者则更需通晓其文字之说明。此外,吾人更须切实互助,     

Petrology and Geochemistry of Post-Collisional Early Miocene Volcanism in the Karacada? Area (Central Anatolia, Turkey)

Early Miocene (ca.?21–18 Ma) volcanism in the Karacada? area comprises three groups of volcanic rocks: (1) calcalkaline suite (andesitic to rhyolitic lavas and their pyroclastics), (2) mildly-alkaline suite (alkali basalt, hawaiite, mugearite, benmoreite and trachydacite), and (3) a single trachyandesitic flow unit. Field observations, 40Ar/39Ar ages and geochemical data show that there was a progressive temporal transition from group 1 to 3 in a post-collisional tectonic setting. The calcalkaline suite rocks with medium-K in composition resemble those of subduction-related lavas, whereas the mildly-alkaline suite rocks having a sodic tendency (Na2O/K2O=1.5–3.2) resemble those of within-plate lavas. Incompatible element and Sr-Nd isotopic characteristics of the suites suggest that the lithospheric mantle beneath the Karacada? area was heterogeneously enriched by two processes before collision: (1) enrichment by subduction-related processes, which is important in the genesis of the calcalkaline volcanism, (2) enrichment by small degree melts from the astenosphere, which dominates the mildly alkaline volcanism. Perturbation of the enriched lithosphere by either delamination following collision and uplift or removal of the subducted slab following subduction and collision (i.e., slab breakoff) is the likely mechanism for the initiation of the post-collision volcanism.     

Resource Flows of Villages with Contrasting Lifestyles in Nanda Devi Biosphere Reserve, Central Himalaya, India

Resource use efficiency analyses of village ecosystem are necessary for effective and efficient planning of resource utilization. This paper deals with economic and energy input-output analyses of different components of village ecosystem in representative buffer zone villages, which are practicing transhumance and settled way of lifestyles in Nanda Devi Biosphere Reserve （NDBR） of Garhwal Himalaya. While the villages practicing transhumance used various natural resources spatially segregated,the villages practicing settled way of lifestyle have to manage resources from a limited spatial area through rotation and varied extraction intensities. Forests subsidized the production activity in both type of villages and the per capita resource extractions were found to be greater in tran~humance village than settled village. Though crops provided maximum energy, in terms of economic criteria, animal husbandry played important role in both settled and transhumance villages. As villages representing both the situations showed different ways of adjustments to the conservation oriented land use changes, management authority needs to address the eco-development plans fulfilling the aspirations of all people traditionally using the resources of the Reserve to reduce the conflicts and encourage their participation in the conservation of the area.     

A Measurement of Omega from the North American Test Flight of Boomerang

We use the angular power spectrum of the cosmic microwave background, measured during the North American test flight of the Boomerang experiment, to constrain the geometry of the universe. Within the class of cold dark matter models, we find that the overall fractional energy density of the universe Omega is constrained to be 0.85相似文献   

ASCA Observation of the Quiescent X-Ray Counterpart to SGR 1627-41

We present a 2-10 keV ASCA observation of the field around the soft gamma repeater SGR 1627-41. A quiescent X-ray source, whose position is consistent both with that of a recently discovered BeppoSAX X-ray source and with the Interplanetary Network localization for this soft gamma repeater, was detected in this observation. In 2-10 keV X-rays, the spectrum of the X-ray source may be fit equally well by a power-law, blackbody, or bremsstrahlung function, with unabsorbed flux approximately 5x10-12 ergs cm-2 s-1. We do not confirm a continuation of a fading trend in the flux, and we find no evidence for periodicity, both of which were noted in the earlier BeppoSAX observations.