A comparison of cytochrome P4501A (CYP1A) mRNA inducibility in four species of Atlantic coast anadromous fishes

Quantification of levels of cytochrome P4501A (CYP1A) gene expression in sentinel species of fishes has been proposed as a management tool to evaluate contamination of aquatic systems. Based on preliminary studies, we hypothesized that differences in CYP1A mRNA inducibility among individuals, populations, or species might lead to spurious conclusions when using this approach in environmental monitoring programs. To address this possibility, we quantitated and compared CYP1A mRNA induction levels in four species of common Atlantic Coast estuarine fish: smooth flounder, hogchoker, striped bass, and Atlantic tomcod, which were treated with model chemicals (beta naphthoflavone (β-NF), or benzo[a]pyrene at 10 ppm) known to induce CYP1A mRNA, or were exposed to contaminated environments. Species-specific CYP1A DNA probes were generated from PCR (polymerase chain reaction) amplification of genomic DNA using conserved oligonucleotide primers, and, along with cloned rainbow trout and Atlantic tomcod CYP1A cDNA probes were used to quantify CYP1A mRNA levels in northern blot analyses. Successful PCR amplification of CYP1A hybridizable DNA fragments was observed for all four species. Results from northern blot analyses showed large differences in CYP1A mRNA induction among species; only Atlantic tomcod exhibited significant induction of CYP1A mRNA for both chemically treated (97-fold) and environmentally exposed fish (34-fold). Significant, although lower, levels of induction were observed in β-NF treated (14-fold) smooth flounder, but not in environmentally exposed smooth flounder. Only low levels (not significant) of CYP1A gene induction were detected in hogchokers and striped bass. We conclude that CYP1A mRNA inducibility differed significantly among fish taxa perhaps due to differences in regulation of gene expression, suggesting that careful selection of sentinel species should be exercised prior to the use of CYP1A mRNA induction in environmental monitoring programs. However, the significance of differences in CYP1A mRNA inducibility in relation to higher level biological endpoints has yet to be determined.     

The transformation and export of phosphorus from Wetlands

There is widespread acceptance of the phosphorus retention capability of wetlands even though research findings are often inconclusive and contradictory. The results of a one year phosphorus budget study indicate that internal wetland processes may transform sediment bound phosphorus to plant available orthophosphorus. While total phosphorus imports were nearly double the total phosphorus exports for the study wetland, orthophosphorus exports were 22 per cent greater than imports. This study supports the recent finding that wetlands have limited capability to retain orthophosphorus and indicates that wetlands may even increase the export of orthophosphorus. The generally accepted nutrient retention function of wetlands and their possible role in eutrophication is thus questionable.     

An analysis of the distribution of boulders in the vicinity of small lunar craters

Nine Orbiter 3 high-resolution photographs were examined at three sites for distributions of boulders around craters 110 m in diameter; three kinds of distributions were noted. Some had boulders inside and far outside (designated), others had boulders only inside or on their ramparts (), and some had no detectable boulders (). Additionally, shapes or morphologies of craters were classified according to their resemblance to one of three Jaffe models (1965); a third classification involved the depth of particulate overlay (Jaffe, 1965). Crater morphologies were classified (A) if they were relatively shallow with sharp, raised rims, (B) if they were rimless and shallow, and (C) if they were relatively deep and had low, broad rims. The data suggested that there were strong relationships between crater types and boulder distributions in which, generally, A-type was related to the-distribution, B-type to-distribution, and C-type to-distribution. Plots of numbers of craters of each crater type and each kind of boulder distribution versus steps of overlay for each site and for the totality of craters considered indicated that neither boulder distribution nor crater type represented orderly progression of age or erosional evolution. The apparent overlays for the A's and C's and for the's and's were similar, implying similar ages or stages of erosion. It is suggested that they represent two kinds of primary craters and that the A's are of impact origin while the C's are of volcanic origin. The B's probably represent later stages of erosion of the C's and possibly of some A's.Now at the University of Maryland.Receipt delayed by postal strike in Great Britain.     

Pre-main sequence stellar evolution with mass loss

A semi-empirical formulation is given of the rate of stellar mass loss by stellar winds. Evolutionary studies of stars in the pre-main sequence (T Tauri) stage are presented for a variety of rates of mass loss. It has been found that different mass loss rates produce only small changes in the positions of equal evolutionary time lines in HR diagrams. Thus it is concluded that the spread of points in HR diagrams of young clusters results from a spread in their times of formation. This is consistent with the initiation of star formation by violent hydrodynamic compression of a typical interstellar cloud.     

Rapid neutron capture in supernova explosions

The implications of recent studies of the dynamics of the cores of highly evolved massive stars are considered with regard to the general problems of nucleosynthesis. The typical conditions estimated for these models are shown to be very promising for the process of element synthesis by neutron capture on a fast time scale (ther-process ofBurbidge et al., 1957).     

Comparison of heat and bromide as ground water tracers near streams

Heat and bromide were compared as tracers for examining stream/ground water exchanges along the middle reaches of the Santa Clara River, California, during a 10-hour surface water sodium bromide injection test. Three cross sections that comprise six shallow (<1 m) piezometers were installed at the upper, middle, and lower sections of a 17 km long study reach, to monitor temperatures and bromide concentrations in the shallow ground water beneath the stream. A heat and ground water transport simulation model and a closely related solute and ground water transport simulation model were matched up for comparison of simulated and observed temperatures and bromide concentrations in the streambed. Vertical, one-dimensional simulations of sediment temperature were fitted to observed temperature results, to yield apparent streambed hydraulic conductivities in each cross section. The temperature-based hydraulic conductivities were assigned to a solute and ground water transport model to predict sediment bromide concentrations, during the sodium bromide injection test. Vertical, one-dimensional simulations of bromide concentrations in the sediments yielded a good match to the observed bromide concentrations, without adjustment of any model parameters except solute dispersivities. This indicates that, for the spatial and temporal scales examined on the Santa Clara River, the use of heat and bromide as tracers provide comparable information with respect to apparent hydraulic conductivities and fluxes for sediments near streams. In other settings, caution should be used due to differences in the nature of conservative (bromide) versus nonconservative (heat) tracers, particularly when preferential flowpaths are present.     

Look out, aurorae about!

Jim Wild recounts the rationale and success of AuroraWatch, a service that alerts you when you might be able to see aurorae.