Biodegradation of <Emphasis Type="Italic">Enteromorpha</Emphasis> polysaccharides by intestinal micro-community from <Emphasis Type="Italic">Siganus oramin</Emphasis>

Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal micro-communities involved in the biodegradation of Enteromorpha polysaccharides (EP) were seldom reported. In order to obtain the EP-degrading micro-community, the intestines of Siganus oramin was obtained to isolate the micro-communities, which were enriched by 0.3% of EP as the sole carbon source. A stable micro-community with EP degradative capability was achieved after seven generations of subculture, named H1. Results showed that H1 was able to degrade 75% of EP within 24 hours, and the activity of EP lyases reached 500 U mL^?1 in 32 hours. With denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis, ten bacteria closely related to Marinomonas pontica, Microbacterium sp., Leucobacter chironomi, Cyclobacterium sp., Algoriphagus winogradskyi, Pseudoalteromonas sp. and Vibrio sp. were determined. Furthermore, compared with the DGGE bands sequence and the clone library analysis, the dominant bacteria of the EP-biodegrading micro- community were Pseudoalteromonas sp. and Vibrio sp., with the respective proportion of 38% and 46%, and they should play an important role in EP degradation together with other degrading bacteria in the micro-community H1.     

Antialgal and antilarval activities of bioactive compounds extracted from the marine dinoflagellate <Emphasis Type="Italic">Amphidinium carterae</Emphasis>

With the global ban on the application of organotin-based marine coatings by the International Maritime Organization, the development of environmentally friendly, low-toxic and nontoxic antifouling compounds for marine industries has become an urgent need. Marine microorganisms have been considered as a potential source of natural antifoulants. In this study, the antifouling potential of marine dinoflagellate Amphidinium carterae, the toxic and red-tide microalgae, was investigated. We performed a series of operations to extract the bioactive substances from Amphidinium carterae and tested their antialgal and antilarval activities. The crude extract of Amphidinium carterae showed significant antialgal activity and the EC₅₀ value against Skeletonema costatum was 55.4 μg mL^?1. After purification, the isolated bioactive substances (the organic extract C) exhibited much higher antialgal and antilarval activities with EC₅₀ of 12.9 μg mL^?1 against Skeletonema costatum and LC₅₀ of 15.1 μg mL^?1 against Amphibalanus amphitrite larvae. Subsequently, IR, Q-TOFMS, and GC-MS were utilized for the structural elucidation of the bioactive compounds, and a series of unsaturated and saturated 16- to 22-carbon fatty acids were detected. The data suggested the bioactive compounds isolated from Amphidinium carterae exhibited a significant inhibiting effect against the diatom Skeletonema costatum and Amphibalanus amphitrite larvae, and could be substitutes for persistent, toxic antifouling compounds.     

The modeled atmospheric and oceanic response to the South China Sea SST anomaly

The sensitivity of the global atmospheric and oceanic response to sea surface temperature anomaly (SSTA) throughout the South China Sea (SCS) is investigated using the Fast Ocean-Atmosphere Model (FOAM). Forced by a warming SST, the experiment explicitly demonstrates that the responses of surface air temperature (SAT) and SST exhibit positive anomalous center over SCS and negative anomalous center over the Northern Pacific Ocean (NPO). The atmospheric response to the warm SST anomalies is characterized by a barotropical anomaly in middle-latitude, leading to a weak subtropical high in summer and a weak Aleutian low in winter. Accordingly, Indian monsoon and eastern Asian monsoon strengthen in summer but weaken in winter as a result of wind convergence owing to the warm SST. It is worth noting that the abnormal signals propagate poleward and eastward away in the form of Rossby Waves from the forcing region, which induces high pressure anomaly. Owing to action of the wind-driven circulation, an anomalous anti-cyclonic circulation is induced with a primary southward current in the upper ocean. An obvious cooling appears over the North Pacific, which can be explained by anomalous meridional cold advection and mixing as shown in the analysises of heat budget and other factors that affect SST.     

Growth performance of larval and juvenile manila clam (<Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>) from divergently selected individuals of a full-sib family

In this study, the method of divergent selection was employed to test the larval and juvenile growth performance within a full-sib family of Manila clam Ruditapes philippinarum. The 10% largest and 10% smallest clam individuals (on the basis of shell length) of a full-sib family were selected as parents for the fast and slow growing lines, respectively. The difference in shell length was significant among the three lines (fast, control, and slow) tested. The sequence of shell length were fast line > control line > slow line. The responses to selection, realized heritability, and genetic gain were 0.06%–0.81%, 0.04%–0.47% and 0.58%–18.89% in the fast direction, respectively; and were 0.14%–1.27%, 0.08%–0.73%, and 0.31%–49.03% in the slow direction, respectively. The results suggested that there was a large portion of additive genetic variance affecting the growth in the full-sib family. Selection in the fast direction within the full-sib family would greatly improve the growth of R. philippinarum.     

The Impact of Large-Scale Reclamation on Hydro-Dynamic Environment——A Case Study of Xinghua Bay

A hydro-dynamic model is established on basis of MIKE21 FM to simulate the hydro-dynamic characteristics of Xinghua Bay and investigate the influence of reclamation project on the tidal elevation and tidal currents. Tidal elevation data was obtained at the six tide gauge stations around the Xinghua Bay, and another six current stations were established to observe the tidal current velocity and direction. Validation shows that the model-simulated tidal elevation and tidal currents agree well with observations made at different stations. Predictions are made according to the reclamation project proposed in the regional marine planning of Hanjiang Industrial Park around the port in Putian City. The variations of hydro-dynamic factors, such as tide, current velocity and direction and tidal influx are obtained, and the adverse effect of reclamation on marine environment is discussed. It is shown that the tidal level inside the Xinghua Bay during high tide decreases after the reclamation project is completed. The tidal currents during flooding tide generally decrease in the southeast of the reclamation region, with the maximum decreasing amplitude reaching 0.44 m s^-1. On the other hand, the tidal currents during flooding tide increase around the southeast and southwest corners of the reclamation region. The tidal currents during ebb tide increase around the southeast and southwest corners of the reclamation region, with the maximum increasing amplitude attaining 0.18 m s^-1. The results in this paper can give some guidance for the marine environment management and the effective utilization of land in Putian.     

Tide and current observations in the central Chukchi Sea during the summer of 2012

Current data from a moored Acoustic Doppler Current Profiler(ADCP) deployed at 69?30.155′N,169?00.654′W in the central Chukchi Sea during 2012 summertime is analyzed in the present paper.Characteristics of tidal and residual currents are ob-tained with Cosine-Lanczos filter and cross-spectral analyses.The main achievements are as follows:1) Along with the local inertial frequency of 12.8 h,two other peaks at ~12-h and ~10-d dominate the time series of raw velocity;2) The M_2 dominates the 6 resolved tide constituents with significant amplitude variations over depth and the ratios of current speed of this constituent to that of the total tidal current are 54% and 47% for u and v components,respectively.All the resolved tidal constituents rotate clockwise at depth with the exception of MM and O1.The constituents of M_2 and S_2 with the largest major semi-axes are similar in eccentricity and orientation at deeper levels;3) The maximum of residual currents varies in a range of 20–30 cms~(-1) over depth and the current with lower velocities flow more true north with smaller magnitudes compared to the current in surface layer.The ~10 d fluctuation of residual current is found throughout the water column and attributed to the response of current to the local wind forcing,with an approximate 1.4 d lag-time at the surface level and occurring several hours later in the lower layer;4) Mean residual currents flow toward the north with the magnitudes smaller than 7 cms~(-1) in a general agreement with previous studies,which suggests a relatively weaker but stable northward flow indeed exists in the central Chukchi Sea.     

Phase characteristic analysis of continuous depth air-gun source wavelet

Air guns are important sources for marine seismic exploration. Far-field wavelet of air gun arrays, as a necessary parameter for pre-stack processing and source models, plays an important role during marine seismic data processing and interpretation. When an air gun fires, it generates a series of air bubbles. Similar to onshore seismic exploration, the water forms a plastic fluid near the bubble; the farther the air gun is located from the measurement, the more steady and more accurately represented the wavelet will be. In practice, hydrophones should be placed more than 100 m from the air gun; however, traditional seismic cables cannot meet this requirement. On the other hand, vertical cables provide a viable solution to this problem. This study uses a vertical cable to receive wavelets from 38 air guns and data are collected offshore Southeast Qiong, where the water depth is over 1000 m. In this study, the wavelets measured using this technique coincide very well with the simulated wavelets and can therefore represent the real shape of the wavelets. This experiment fills a technology gap in China.     

Provenance of buried esker groundwater: the case of Vars-Winchester esker aquifer,Eastern Ontario,Canada

An innovative mode of groundwater recharge to a buried esker aquifer is considered. The current conceptual model affords a natural safeguard to underlying aquifers from the overlying muds. A hypothesis of groundwater recharge to a buried esker aquifer via preferential pathways across its overlying muds is tested here by heuristic numerical one-dimensional and two-dimensional modeling simulations. The hypothesis has been tested against two other conventionally accepted scenarios involving: (1) distal esker outcrop areas and (2) remote shallow-bedrock recharge areas. The main evidence comes from documented recharge pressure pulses in the overlying mud aquitard and in the underlying esker hydraulic-head time series for the Vars-Winchester esker aquifer in Eastern Ontario, Canada. These perturbations to the potentiometric surface are believed to be the aquifer response to recharge events. The migration rate of these pressure pulses is directly related to the hydraulic diffusivity of the formation. The measured response time and response amplitude between singular radar precipitation events and well hydrographs constituted the heuristic model calibration targets. The main evidence also includes mud-layering deformation (water escape features) which was observed in seismic surveys of the over-esker muds. These disturbed stratigraphic elements provide a realistic mechanism for migrating water to transit through the muds. The effective hydraulic conductivities of these preferential pathways in the muds were estimated to be between 2?×?10^?6 and 7?×?10^?6 m/s. The implications of these findings relate to the alleged natural safeguard of these overlying muds.     

新疆东准乌须克劳格岩体LA-ICP-MS锆石U-Pb年龄、Hf同位素特征及其地质意义

新疆东准噶尔琼河坝地区近年来找矿获得重大突破,发现了一系列重要的矿产,这些矿产基本上围绕该地区出露面积最大的乌须克劳格岩体分布。该岩体组成比较复杂,主要岩性为二长花岗岩和花岗闪长岩。采用LA-ICP-MS技术测得花岗闪长岩和二长花岗岩锆石~(206)Pb/~(238)U年龄分别为428.9±1.9Ma和427.2±1.7Ma,被解释为岩石的结晶年龄。2类岩石都属于过铝质-钙碱性系列花岗岩,具有较高的铝、钙含量,K_2O/Na_2O值普遍偏低,介于0.15~0.40之间;稀土元素配分曲线呈现右倾特征,稀土元素总量较低,轻稀土元素相对富集,负Eu异常不明显;在微量元素原始地幔标准化蛛网图上,高场强元素Th、Nb、Ta、P、Ti等相对亏损,大离子亲石元素Rb、Ba、U、K、Sr等相对富集,具有高Ba、Sr含量和高(La/Yb)_N、Sr/Y值,显示出高Ba-Sr花岗岩的特征;同时岩体具有高正ε_(Hf)(t)值和年轻的模式年龄。这些地球化学和同位素特征表明,晚志留世乌须克劳格岩体是大陆边缘弧环境下大洋板片熔融的产物。     

天水盆地新近纪伸展构造——来自沉积与构造变形方面的证据

天水盆地位于青藏高原东北缘高海拔挤压隆升区与鄂尔多斯低海拔伸展区的过渡部位,新构造活动强烈。然而,新构造活动对天水盆地的影响尚不清楚。通过对盆地的沉积环境、构造沉降、构造变形等方面的研究,结果显示:1盆地由风成堆积、洪积扇、河湖相与湖泊相沉积组成,代表沉积中心的河湖相与湖泊相沿控盆断裂(西秦岭北缘断裂、西和断裂与礼县-罗家堡断裂)分布;2盆地经历了16~14Ma、9.2~7.4Ma和3.6~2.6Ma三次加速沉降期;3控盆断裂在同沉积期为正断层。沉积中心沿断裂分布、快速沉降事件及生长正断层表明,天水盆地至少在中新世晚期受控于走滑伸展构造,记录了青藏高原向北东方向的构造挤出作用。