Analysis of mutations in the p53 tumor suppressor gene and Ki- and Ha-ras proto-oncogenes in hepatic tumors of European flounder (Platichthys flesus).

DNA from five tumors, three other-injured livers and two normal liver tissue samples from the European flounder were analyzed for mutations in exons 5-8 of the tumor suppressor gene p53 and at codons 12, 13 and 61 of Ha- and Ki-ras proto-oncogenes. No tumor-specific mutations were identified by direct sequencing and single-strand conformation polymorphism of these genes. A number of silent polymorphisms were noted in p53. In addition to a need for more extensive analyses of flounder liver tumor samples for ras or p53 mutations, other cancer-related genes should be investigated.     

Improved Resolution of Ambient Flow through Fractured Rock with Temperature Logs

In contaminant hydrogeology, investigations at fractured rock sites are typically undertaken to improve understanding of the fracture networks and associated groundwater flow that govern past and/or future contaminant transport. Conventional hydrogeologic, geophysical, and hydrophysical techniques used to develop a conceptual model are often implemented in open boreholes under conditions of cross-connected flow. A new approach using high-resolution temperature (±0.001°C) profiles measured within static water columns of boreholes sealed using continuous, water-inflated, flexible liners (FLUTe™) identifies hydraulically active fractures under ambient (natural) groundwater flow conditions. The value of this approach is assessed by comparisons of temperature profiles from holes (100 to 200 m deep) with and without liners at four contaminated sites with distinctly different hydrogeologic conditions. The results from the lined holes consistently show many more hydraulically active fractures than the open-hole profiles, in which the influence of vertical flow through the borehole between a few fractures masks important intermediary flow zones. Temperature measurements in temporarily sealed boreholes not only improve the sensitivity and accuracy of identifying hydraulically active fractures under ambient conditions but also offer new insights regarding previously unresolvable flow distributions in fractured rock systems, while leaving the borehole available for other forms of testing and monitoring device installation.     

Diatom evidence for autochthonous artifact deposition in the Valsequillo region, Puebla, Mexico during the Sangamonian (sensu lato = 80,000 to ca. 220,000 yr BP and Illinoian (220,000 to 430,000 yr BP))

Fossil diatoms in the Valsequillo area are important in supplying adequate paleoecological evidence for the in situ deposition (in the absence of strong water currents necessary for the displacement and redeposition) of artifacts as large as those at the Hueyatlaco Archaeological Site. The paleoecology of lacustrine diatom-bearing samples from four nearby localities in the Valsequillo region all correlated with numerous diatomaceous samples from the Hueyatlaco Site (Puebla, Mexico) and indicate an autochthonous deposition of the artifacts at that site. This correlative evidence is consistent with a deposition in Sangamonian to Illinoian time and is based on the relationships of percentages of taxa in categories of the current, pH, and halobian spectra in six lines of correlation of samples between the Hueyatlaco Site and the four localities.     

Impact of Sea-level Rise and Storm Surges on a Coastal Community

A technique to evaluate the risk of storm tides (the combination of a storm surge and tide) under present and enhanced greenhouse conditions has been applied to Cairns on the north-eastern Australian coast. The technique combines a statistical model for cyclone occurrence with a state-of-the-art storm surge inundation model and involves the random generation of a large number of storm tide simulations. The set of simulations constitutes a synthetic record of extreme sea-level events that can be analysed to produce storm tide return periods. The use of a dynamic storm surge model with overland flooding capability means that the spatial extent of flooding is also implicitly modelled. The technique has the advantage that it can readily be modified to include projected changes to cyclone behaviour due to the enhanced greenhouse effect. Sea-level heights in the current climate for return periods of 50, 100, 500 and 1000 years have been determined to be 2.0 m, 2.3 m, 3.0 m and 3.4 m respectively. In an enhanced greenhouse climate (around 2050), projected increases in cyclone intensity and mean sea-level see these heights increase to 2.4 m, 2.8 m, 3.8 m and 4.2 m respectively. The average area inundated by events with a return period greater than 100 years is found to more than double under enhanced greenhouse conditions.     

The British Tertiary igneous province: palaeomagnetism of the dyke swarm along the Sleat coast of Skye

Summary. A palaeomagnetic investigation has been made of a swarm of more than 400 dykes along the south coast of Skye, Scotland, by the Sound of Sleat.
Seven red lamprophyre dykes have palaeomagnetic directions inconsistent with Tertiary age, and not inconsistent with their previously held Caledonian age. The remaining 409 dykes have palaeomagnetic directions that are consistent with a Lower Tertiary age. We present evidence suggesting that the Tertiary dykes might have been emplaced during a short time, over which the geomagnetic polarity occupied as few as three polarity intervals (NRN or RNR).
Certain 'intermediate' directions of magnetization have also been found, and are presented here.     

Palaeomagnetism of the teschenitic rocks (Lower Cretaceous) in the Outer Western Carpathians of Poland: constraints for tectonic rotations in the Silesian unit

Results of palaeomagnetic investigations of the Lower Cretaceous teschenitic rocks in the Silesian unit of the Outer Western Carpathians in Poland bring evidence for pre-folding magnetization of these rocks. The mixed-polarity component reveals inclinations, between 56° and 69°, which might be either of Cretaceous or Tertiary age. Apparently positive results of fold and contact tests in some localities and presence of pyrhotite in the contact aureole suggest that magnetization is primary, although a Neogene or earlier remagnetization cannot be totally excluded since inclination-only test between localities gives 'syn-folding' results. Higher palaeoinclinations (66°–69°) correlate with a younger variety of teschenitic rocks dated for 122–120 Ma, while lower inclinations (56°–60°) with an older variety (138–133 Ma). This would support relatively high palaeolatitudes for the southern margin of the Eurasian plate in the late part of the Early Cretaceous and relatively quick northward drift of the plate in this epoch, together with the Silesian basin at its southern margin. Declinations are similar to the Cretaceous–Tertiary palaeodeclinations of stable Europe in the eastern part of the studied area but rotated ca. 14°–70° counter-clockwise in the western part. This indicates, together with older results from Czech and Slovakian sectors of the Silesian unit, a change in the rotation pattern from counter-clockwise to clockwise at the meridian of 19°E. The rotations took place before the final collision of the Outer Carpathians nappe stack with the European foreland.     

Local provenance in rehabilitation of degraded landscapes: a case study from the Hawkesbury–Nepean catchment, Australia

Throughout Australia thousands of volunteers are engaged in Landcare projects that should help rehabilitate degraded landscapes. Many of these projects involve tree planting, but their seed is not necessarily of local provenance. Based on a survey of 85 Landcare groups working in the Hawkesbury–Nepean catchment, data were collected about Landcare groups' knowledge of their seed source, understanding of local provenance and the ecosystem in which they were planting trees and the source of funding for their projects. The findings from the study indicate that about one in five (21%) of the groups surveyed that introduced plant material were not aware of local provenance issues. Indirect indications were that a large number of Landcare groups state-wide may be doing more harm than good to the landscape while trying to rehabilitate it. The data also showed that one in seven (13%) of the groups funded by the Australian Government through the Natural Heritage Trust (NHT) had limited awareness of local provenance issues. With millions of dollars being spent on 'works on the ground', it would be prudent to allocate some funds to document and monitor current Landcare activities, so that the environmental outcomes can be quantified and more effective Landcare policies can be developed in the future.