Assessing Cryptic Invasion State: Fine-Scale Genetic Analysis of Ciona savignyi Population in Putative Native Habitat of the Korean Coast

The solitary ascidian, Ciona savignyi (Ascidiacea, Enterogona) is a notorious marine invader still expanding its habitat range worldwide. This species is considered native to the North West Pacific, but its indigeneity in Korean coastal waters has been questioned because of outdated taxonomic records and its inhabitation of oceanographically marginal areas. To clarify their cryptic invasion state, 247 individual C. savignyi samples were collected from 12 harbors and marinas on the Korean coast, and a 744 bp region of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I gene was sequenced and analyzed. Our analyses of population genetic structure and demographic history provided considerable pieces of evidence supporting their long-term establishment on the Korean coasts: differentiated population genetic structure, sequentially arrayed star-shape haplotype network, neutrality test results of past population expansions, and post-glacial colonization pattern of demography. Consequently, we concluded that C. savignyi populations on the Korean Coast are indigenous rather than exotic. These results could be used as reference data for further phylogeo graphic and demographic studies of problematic Ciona species, and to clarify and resolve similar cryptic invasion states of the other Korean coastal marine organisms. This study is the first to resolve the cryptic in vasion state of Korean marine organisms using genetic analysis.

     

基于乡村多功能评价的城市边缘区“三生”空间划分研究

城市边缘区乡村多元价值凸显,迫切需以乡村多功能理论为指导划分“三生”空间,以空间重构促进转型发展。论文首先从生产、生活与生态功能方面评价城市边缘区多功能时空变化;其次进行图斑尺度“三生”空间适宜性评价,两者共同得到基于乡村多功能的“三生”空间适宜性;最后据此设计算法划分“三生”空间。研究发现：① 城市边缘区乡村休闲娱乐、观光农业等功能显现,需要重构多功能空间促进转型发展;② 江苏徐州市铜山区乡村非农生产与生活功能主要分布在与中心城区较近地区,农业空间主要分布在中心城区远郊外围,生态功能则表现出高度空间异质性,农业、非农生产与生活功能在提升,而生态功能则普遍下降;③ 县域“三生”空间划定需多尺度联合,既要考虑乡镇尺度多功能差异,还要体现图斑尺度适宜性,才能为空间规划提供有意义的借鉴。论文提出的基于乡村多功能评价的“三生”空间划分可为即将开展的国土空间规划提供科学依据。     

Cryopreservation of Veliger Larvae of Trumpet Shell,Charonia sauliae: an Essential Preparation to Artificial Propagation

Trumpet shell, Charonia sauliae, is an endangered and valuable species, but its artificial propagation protocol has not been successfully established. To estimate the possibility of cryopreservation for larvae of C. sauliae, which is a potential preparation for its artificial reproduction and further research, in this study a protocol for the cryopreservation of veliger larvae of trumpet shell was optimized. Through a two-step cryopreservation procedure, four kinds of cryoprotectants (ethylene glycol, 1, 2-propanediol,dimethyl sulfoxide and glycerol) were employed at three concentrations (1.0, 1.5 and 2.0 molL-1) respectively and survival rates of larvae were determined after a storage of 1h. The larvae frozen with these four cryoprotectants after 1h storage were cultured, and then survival rates were determined at 24, 72 and 120h after thawing. Dimethyl sulfoxide at a concentration of 1.5 molL-1 showed the best protective effect in all experiments (p<0.05). And survival rates of larvae frozen with dimethyl sulfoxide were determined after 1,7 and 15 d of storage. The survival rates of larvae frozen with 1.5 molL-1 dimethyl sulfoxide after 1h, 1d, 7 d and 15 d of storage were 80.77% ±7.51%, 80.34% ±11.28%, 83.10% ±9.14% and 77.23%±6.22% respectively. No significant differences in survival rates of larvae frozen with dimethyl sulfoxide were observed after various storage periods (p>0.05).     

Contrast in life histories of exploited fishes and ecosystem structures in coastal waters off west Canada and east Korea

By reviewing the history of fishery exploitation in the coastal waters of west Canada and east Korea, related with contrasting life history strategies of the dominant species, the fishery management challenges that each country would face in the upcoming decades were outlined. In the ecosystem of the Canadian western coastal waters, the dominant oceanographic feature is the coastal upwelling domain off the west coast of Vancouver Island, the northernmost extent of the California Current System in the eastern North Pacific. In the marine ecosystem of the eastern coasts of Korea (the Japan/East Sea), a major oceanographic feature is the Tsushima Warm Current, a branch of the Kuroshio Current in the western North Pacific. Fishes in the Canadian ecosystem are dominated by demersal, long-lived species such as flatfish, rockfish, sablefish, and halibut. During summer, migratory pelagic species such as Pacific hake, Pacific salmon, and recently Pacific sardine, move into this area to feed. In the late 1970s, Canada declared jurisdiction for 200 miles from their coastline, and major fisheries species in Canadian waters have been managed with a quota system. The overall fishing intensity off the west coast of Vancouver Island has been relatively moderate compared to Korean waters. Fishes in the ecosystem of the eastern Korean waters are dominated by short-lived pelagic and demersal fish. Historically, Korea has shared marine resources in this area with neighbouring countries, but stock assessments and quotas have only recently (since the late-1990s) been implemented for some major species. In the Korean ecosystem, fisheries can be described as intensive, and many stocks have been rated as overfished. The two ecosystems responded differently to climate impacts such as regime shifts under different exploitation histories. In the future, both countries will face the challenge of global warming and subsequent impacts on ecosystems, necessitating developing adaptive fisheries management plans. The challenges will be contrasting for the two countries: Canada will need to conserve fish populations, while Korea will need to focus on rebuilding depleted fish populations.     

Review of recent findings on the water masses and circulation in the East Sea (Sea of Japan)

Recent findings on water masses, biogeochemical tracers, deep currents and basin-scale circulation in the East/Japan Sea, and numerical modeling of its circulation are reviewed. Warming continues up to 2007 despite an episode of bottom water formation in the winter of 2000–2001. Water masses have definitely changed since the 1970s and further changes are expected due to the continuation of warming. Accumulation of current data in deep waters of the East/Japan Sea reveals that the circulation in the East/Japan Sea is primarily cyclonic with sub-basin scale cyclonic and anticyclonic cells in the Ulleung Basin (Tsushima Basin). Our understanding of the circulation of intermediate water masses has been deepened through high-resolution numerical studies, and the implementation of data assimilation has had initial success. However, the East/Japan Sea is unique in terms of the fine vertical structures of physical and biogeochemical properties of cold water mass measured at the highest precision and their rapid change with the global warming, so that full understanding of the structures and their change requires in-depth process studies with continuous monitoring programs.     

Cadmium and phosphorus cycling in the water column of the South China Sea: The roles of biotic and abiotic particles

The concentrations of cadmium, phosphorus, and aluminum in size-fractionated phytoplankton, zooplankton, and sinking particles are determined using ICPMS to evaluate the roles of biotic and abiotic particles on the cycling and ratios of Cd and P in the water column. Plankton were collected with a filtration apparatus equipped with 10-, 60-, and 150-μm aperture plankton nets on two occasions (2002 and 2006), and sinking particles were sampled by moored sediment traps deployed at depths of 120, 600, and 3500 m from 2004 to 2005. In contrast to what our previous study revealed, i.e., that most of the other bioactive trace metals in plankton were strongly correlated with abiotic Al and adsorbed on phytoplankton [Ho, T.Y., Wen, L.S., You, C.F., Lee, D.C., 2007. The trace metal composition of size-fractionated plankton in the South China Sea: biotic versus abiotic sources. Limnol Oceanogr 52, 1776–88.], Cd/P ratios, ranging from 0.12 to 0.34 mmol/mol P, did not vary with Al and exhibited fairly consistent values among different sizes of plankton, showing that Cd was mostly incorporated on an intracellular basis. In terms of the sinking particles, fluxes in Cd and P as well as in Cd/P ratios were strongly influenced by both biotic and abiotic particles. Overall, the Cd/P ratios in the sinking particles ranged from 0.03 to 1.2 mmol/mol, with the highest value observed in traps at 120 m during the productive season. The lowest value was observed in deep water during high flux periods for lithogenic particles. At surface depth, flux and Cd/P ratios were elevated during the most productive season in the region. The elevated ratios in the traps at 120 m were most likely related to preferential uptake of Cd for the dominant species (coccolithophores) during the productive period. Relatively, Cd/P ratios sharply decreased with increasing Al flux in deep water and ratios were much lower than the expected Cd/P ratios obtained from the relative portion of lithogenic and biogenic particles, indicating that the adsorption of soluble P into lithogenic particles was significant in the deep water during high lithogenic particle flux periods. Using averaged annual fluxes and standing stock in the water column, the residence time of biogenic Cd and P are 0.10 and 0.20, 250 and 100, and 9100 and 5000 years respectively in the top 120 m, 600 m, and water column as a whole, also showing preferential removal for Cd in the euphotic zone but relatively higher removal rates for P in the deep water. Our study suggests that the shift in microalgal community structure along with input of lithogenic minerals are both potentially important factors in influencing Cd/P ratios in oceanic water on a geological time scale.     

Small-scale marine fisheries policy in Vietnam

Vietnam's marine fisheries are considered to be small scale and are concentrated in coastal near-shore waters. This has resulted in heavy pressure on near-shore fisheries resources. Near-shore fisheries are considered by fishers and the government to be over-exploited, causing hardship for many coastal communities. This paper reviews and analyzes changes in policy towards small-scale fisheries in Vietnam over the last two decades. The primary issues facing the small-scale fisheries in Vietnam are to restructure the near-shore fisheries and to address over-capacity. Recommended actions include improved fisheries statistics, resources for provincial fisheries staff, and a coordinated and integrated approach involving a mixed strategy of resource management; resource restoration; economic and community development; and new governance arrangements.     

Combating piracy and armed robbery in Asia: The ReCAAP Information Sharing Centre (ISC)

The Regional Cooperation Agreement on Combating Piracy and Armed Robbery Against Ships in Asia (ReCAAP) Information Sharing Centre (ISC) is an international organisation that comprises 14 member countries in the region. Despite its many advantages, the ISC has some weaknesses, which when addressed could bring regional cooperation to a new level and which could make the ISC a model for other regions.