Rapid,specific and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification targeted to vvhA gene

Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification(LAMP) technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.     

Newly discovered hydrothermal fields along the ultraslow-spreading Southwest Indian Ridge around 63°E

The ultraslow-spreading Southwest Indian Ridge(SWIR) to the east of the Melville fracture zone is characterized by very low melt supply and intensive tectonic activity. Due to its weak thermal budget and extremely slow spreading rate, the easternmost SWIR was considered to be devoid of hydrothermal activity until the discovery of the inactive Mt. Jourdanne hydrothermal field(27°51′S, 63°56′E) in 1998. During the COMRA DY115-20 cruise in2009, two additional hydrothermal fields(i.e., the Tiancheng(27°51′S, 63°55′E) and Tianzuo(27°57′S, 63°32′E)fields) were discovered. Further detailed investigations of these two hydrothermal sites were conducted by Chinese manned submersible Jiaolong in 2014–2015. The Tiancheng filed can be characterized as a lowtemperature(up to 13.2°C) diffuse flow hydrothermal field, and is hosted by fractured basalts with hydrothermal fauna widespread on the seafloor. The Tianzuo hydrothermal field is an inactive sulfide field, which is hosted by ultramafic rocks and controlled by detachment fault. The discovery of the three hydrothermal fields around Segment #11 which receives more melt than the regional average, provided evidence for local enhanced magmatism providing heat source to drive hydrothermal circulation. We further imply that hydrothermal activity and sulfide deposits may be rather promising along the easternmost SWIR.     

热带西太平洋砂壳纤毛虫的多样性

为了探明热带西太平洋海区砂壳纤毛虫的多样性、垂直分布及纬向变化,我们于2012年11-12月在该海区四个断面采集了0-200 m的水样。共检出砂壳纤毛虫39属124种,多数种类喜好生活在表层和次表层。砂壳纤毛虫种丰富度、丰度以及多样性指数的高值主要出现在比叶绿素浓度最大值稍浅的位置,与温度和叶绿素浓度显著正相关,与盐度和采样深度显著负相关。多数优势种与环境因子间的相关性不显著。热带西太平洋海区砂壳纤毛虫多样性极高,各站种丰富度在25-52之间。在从表层至75 m处的多数采样点香农指数均大于3。该海区砂壳纤毛虫冗余种的比例很高,占砂壳纤毛虫总种类数的87.90%,贡献了砂壳纤毛虫60.38%的丰度,表明热带西太平洋海区砂壳纤毛虫群落对饵料组成和摄食压力改变的应对能力很强,稳定性很高。     

不同台风合成风场方案在南海的适用性研究

我国南海地区常受台风侵扰,台风场是研究台风浪灾害以及风暴增水灾害的必要条件之一。因遥感风场存在台风大风圈附近风速明显偏小的问题,所以在台风场相关研究中,多采用台风模型风场和背景风场相结合的方式——即合成风场。本文选取南海相关研究中常用的3种台风场模型,对比其特点,并采用NECP CFSR作为背景风场,将合成风场和实际观测风速进行对比,结果显示在南海地区采用Jelesnianski模型得出的风场和实际风场吻合度最好,建议在该风场基础上考虑南海的地理特殊性,进行台风场模型的改进。     

大连市建设我国北方自由贸易港的可行性

在"一带一路"建设、我国东北振兴和深化发展自由贸易试验区的背景下,文章分析大连市建设我国北方自由贸易港的可行性。大连市具有自然和历史优势、区位和交通优势以及政策优势,同时面临港口竞争激烈、国际政治影响、科技成果转化率低和创新人才流失的挑战;未来应在发挥各类优势的基础上,学习和对接上海市建设自贸区的经验,做好区域协调分工,加速产业转型升级,拓展对外贸易范围,加强区港联动,重视科技成果本地转化和创新人才培养引进,全力打造新时期的自由贸易港。     

Detrital apatite fission track constraints on Cenozoic tectonic evolution of the northeastern Qinghai-Tibet Plateau,China: Evidence from Cenozoic strata in Lulehe section,Northern Qaidam Basin

The Northern Qaidam Basin is located at the northeastern part of the Qinghai-Tibetan Plateau. It contains very thick Cenozoic terrestrial clastic sediments, which records the formation of the northern Qaidam Basin due to compressional deformation during the Indo-Asian collision. In this paper, we used detrital apatite fission-track thermochronology, including 4 sandstones and 2 conglomerates samples from the Lulehe section, to reveal the Cenozoic evolution of the northern Qaidam Basin. Fission-track dating indicated the source region of the Lulehe section has experienced important cooling and uplifting in the Late Cretaceous (at ~85.1 Ma and ~65 Ma) and the Eocene (~52 Ma), respectively. The AFT age distribution on the section suggested that the provenance of Lulehe section sediments were mainly derived from the south Qilian Shan (Qilian Mountains) and Altun Shan (Altun Mountains), and two significantly provenance changes may occur at 43.4-46.1 Ma and ~37.8 Ma, respectively. The results may have strong constrains on the Cenozoic deformation and tectonic evolution of the northern Qaidam Basin and Qinghai-Tibet Plateau.     

PS Vir:a short-period solar-like contact binary

We present multi-color photometric observations and a one-dimensional spectrum, acquired from March 2016 to May 2017, for the short-period eclipsing binary PS Vir, by using the 2.16-m,85-cm and 60-cm telescopes at Xinglong station, which is administered by National Astronomical Observatories, Chinese Academy of Sciences. The spectral type was determined as G2V from the onedimensional spectrum. The photometric solution was reduced from BV Rc light curves. The results imply that PS Vir is a W-subtype contact binary with a mass ratio of q = 0.305(±0.008) and a fill-out factor of f = 14.4(±1.8)%. The orbital period may be undergoing a cyclic oscillation with an amplitude of A = 0.0027(±0.0001) d and a modulated period of 11.7(±0.2) yr, which may result from the light-time effect due to a third body. The lower limit on mass for the assumed component is 0.12 M⊙.Moreover, the more massive component of PS Vir may be a bit more evolved star as determined from the mass-luminosity diagram.     

Binary Star Fractions from the LAMOST DR4

Stellar systems composed of single, double, triple or higher-order systems are rightfully regarded as the fundamental building blocks of the Milky Way. Binary stars play an important role in formation and evolution of the Galaxy. Through comparing the radial velocity variations from multiepoch observations, we analyze the binary fraction of dwarf stars observed with LAMOST. Effects of different model assumptions, such as orbital period distributions on the estimate of binary fractions,are investigated. The results based on log-normal distribution of orbital periods reproduce the previous complete analyses better than the power-law distribution. We find that the binary fraction increases with Teff and decreases with [Fe/H]. We first investigate the relation between α-elements and binary fraction in such a large sample as provided by LAMOST. The old stars with high [α/Fe] dominate with a higher binary fraction than young stars with low [α/Fe]. At the same mass, earlier forming stars possess a higher binary fraction than newly forming ones, which may be related with evolution of the Galaxy.     

LAMOST CCD camera-control system based on RTS2

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST) is the largest existing spectroscopic survey telescope, having 32 scientific charge-coupled-device(CCD) cameras for acquiring spectra. Stability and automation of the camera-control software are essential, but cannot be provided by the existing system. The Remote Telescope System 2 nd Version(RTS2) is an open-source and automatic observatory-control system. However, all previous RTS2 applications were developed for small telescopes. This paper focuses on implementation of an RTS2-based camera-control system for the 32 CCDs of LAMOST. A virtual camera module inherited from the RTS2 camera module is built as a device component working on the RTS2 framework. To improve the controllability and robustness, a virtualized layer is designed using the master-slave software paradigm, and the virtual camera module is mapped to the 32 real cameras of LAMOST. The new system is deployed in the actual environment and experimentally tested. Finally, multiple observations are conducted using this new RTS2-frameworkbased control system. The new camera-control system is found to satisfy the requirements for automatic camera control in LAMOST. This is the first time that RTS2 has been applied to a large telescope, and provides a referential solution for full RTS2 introduction to the LAMOST observatory control system.     

An algorithm to resolve γ-rays from charged cosmic rays with DAMPE

The DArk Matter Particle Explorer(DAMPE),also known as Wukong in China,which was launched on 2015 December 17,is a new high energy cosmic ray and γ-ray satellite-borne observatory.One of the main scientific goals of DAMPE is to observe Ge V-Te V high energy γ-rays with accurate energy,angular and time resolution,to indirectly search for dark matter particles and for the study of high energy astrophysics. Due to the comparatively higher fluxes of charged cosmic rays with respect to γ-rays,it is challenging to identify γ-rays with sufficiently high efficiency,minimizing the amount of charged cosmic ray contamination. In this work we present a method to identify γ-rays in DAMPE data based on Monte Carlo simulations,using the powerful electromagnetic/hadronic shower discrimination provided by the calorimeter and the veto detection of charged particles provided by the plastic scintillation detector. Monte Carlo simulations show that after this selection the number of electrons and protons that contaminate the selected γ-ray events at～10 Ge V amounts to less than 1% of the selected sample.Finally,we use flight data to verify the effectiveness of the method by highlighting known γ-ray sources in the sky and by reconstructing preliminary light curves of the Geminga pulsar.