玻尿酸合成酶的鲨鱼源单域抗体筛选

在鲨鱼等软骨鱼类体内存在天然的缺失轻链、仅包含重链的抗体,源于这种重链抗体可变区的片段称为单域抗体(single domain antibody)。目前单域抗体研究中的抗原主要来源于水溶性蛋白或病原体,筛选膜蛋白鲨鱼源单域抗体的领域接近空白。本研究以重组表达的小球藻病毒玻尿酸合成酶(一种膜蛋白)为抗原,经过免疫、建库、淘选、验证等步骤获得了抗原特异性条纹斑竹鲨(Chiloscyllium plagiosum)单域抗体序列。随后利用大肠杆菌表达了该单域抗体,通过等温滴定量热技术(ITC)测定了其与抗原的亲和力,证实了以膜蛋白作为抗原制备鲨鱼源单域抗体的可行性。     

黄海、东海区域漫衰减系数光谱遥感反演及激光测深性能评估

本文在总结和比较中国现有黄海、东海海域漫衰减系数反演算法的基础上,利用水色测量数据分析得到漫衰减系数Kd(532)与Kd(490)之间的关系,为激光测深系统(以CZMIL为例)的性能评估提供了基础。利用MODIS二级数据产品评估了中国黄海、东海海域在CZMIL海道测量模式下最大可测水深的空间分布。研究结果表明CZMIL在黄海、东海海域可测水深基本在0—50 m,该区域的面积占研究区域面积的比例为76.2%,为浅海海域开展激光测深作业提供了测深能力评估的依据和方法。     

Effect of Length Scale Tuning of Background Error in WRF-3DVAR System on Assimilation of High-Resolution Surface Data for Heavy Rainfall Simulation

We investigated the impact of tuning the length scale of the background error covariance in the Weather Research and Forecasting(WRF) three-dimensional variational assimilation(3DVAR) system.In particular,we studied the effect of this parameter on the assimilation of high-resolution surface data for heavy rainfall forecasts associated with mesoscale convective systems over the Korean Peninsula.In the assimilation of high-resolution surface data,the National Meteorological Center method tended to exaggerate the length scale that determined the shape and extent to which observed information spreads out.In this study,we used the difference between observation and background data to tune the length scale in the assimilation of high-resolution surface data.The resulting assimilation clearly showed that the analysis with the tuned length scale was able to reproduce the small-scale features of the ideal field effectively.We also investigated the effect of a double-iteration method with two different length scales,representing large and small-length scales in the WRF-3DVAR.This method reflected the large and small-scale features of observed information in the model fields.The quantitative accuracy of the precipitation forecast using this double iteration with two different length scales for heavy rainfall was high;results were in good agreement with observations in terms of the maximum rainfall amount and equitable threat scores.The improved forecast in the experiment resulted from the development of well-identified mesoscale convective systems by intensified low-level winds and their consequent convergence near the rainfall area.     

Tracking historical storm records from high-barrier lagoon deposits on the southeastern coast of Hainan Island,China

The relationship between storm activity and global warming remains uncertain. To better understand storm–climate relationships, coastal lagoon deposits are increasingly being investigated because they could provide high-resolution storm records long enough to cover past climate changes. However, site-specific sediment dynamics and high barriers may bias storm reconstructions. Here, we aimed to investigate these factors through the reconstruction of five distinct storm records (XCL-01, XC-03, XC-06, XC-07, XC-08) from different water depths in a lagoon with a high barrier (i.e., Xincun Lagoon of Hainan Island). Sediment cores were characterized using high-resolution grain size and XRF measurements, to identify storm events. These data were coupled with a numerical simulation to obtain bed shear stress data with high-spatial resolution to better understand storm-induced sediment transport mechanisms. 210Pb dating and Pb pollution chronostratigraphic markers indicated that the chronology of the storm deposit sequences of the cores span the period between 117 a and 348 a. The grain size and XRF results indicated numerous, highly variable and short-duration fluctuations, suggesting that storm-induced coarse-grained sediments were deposited at these core sites. The inconsistent storm events recorded in these cores suggest that these sites have different preservation potentials for storm deposits. However, the consistence between storm sediment records and historical documents for Core XCL-01 indicates that high-barrier lagoons could provide long-term storm event records with high preservation potential.     

Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast,the Yellow Sea,China: a metabarcoding approach

Journal of Oceanology and Limnology - Surface sediment samples were collected in three different functional sea areas in Qingdao coast, East China, including the inner Jiaozhou Bay, the Laoshan...     

烟台市牟平区废弃矿山地质环境问题及生态修复治理措施

本文以烟台市牟平区某废弃矿山为例,介绍了该区域存在的地质环境问题,分析了造成该区域矿山地质环境破坏的原因,以恢复耕地、草地、林地为目的,对区内的现状进行分析,对采坑、残丘、料场提出相应的修复方案。矿山地质环境恢复治理需从科学的角度树立正确的修复理念,落实地貌重塑、土壤重构和植被恢复三大矿山修复核心技术,为矿山地质环境保护与生态恢复治理提供重要的科学依据。     

震源和水听器沉放深度对单道地震勘探的影响

为研究震源和水听器电缆沉放深度对单道地震勘探的影响,建立鬼波影响模型,采用雷克子波模拟震源子波,分析因震源及水听器电缆沉放深度产生的鬼波对一次有效反射地震波的影响,并采用不同震源和水听器电缆沉放深度的数据对本次研究结论进行验证。研究表明：地震剖面分辨率和震源及水听器电缆沉放的总深度呈反比;随着震源或水听器电缆沉放深度的增加,地震剖面的分辨率递减,接收地震波能量从低值递增到最大值,再从最大值缓慢降低到稳定值;当水听器电缆沉放深度小于168.8/f(m)时,接收地震波能量随震源沉放深度变化的波动相对平缓,水听器电缆沉放深度浅有利于地震波的一致性,但沉放深度过浅时接收地震波能量将被极大削弱,会明显降低地震波的有效穿透深度;当震源沉放深度约为292.3/f(m)时,一次有效反射波的峰值和震源鬼波的峰值重叠,接收地震波能量最大;当震源沉放深度大于584.6/f(m)时,不考虑地震波能量在水中衰减的情况下,震源沉放深度继续增加对接收地震波能量的增加无效。     

丰镇井水位变化与降雨量的关系研究

在结合丰镇井区域水文地质条件的基础上,分析了井水位变化与降雨量的关系。研究结果表明：开展水位（年初值、年末值、年极大值、年极小值、年际变幅和极值变幅）与年降雨量的关系研究,对区域震情的跟踪和判定具有十分重要意义。     

A case study: travel time inversion for P-wave velocity using OBS data of South China Sea

It is very important for converting the seismic data from the time domain to the depth domain. Here we discuss the approaches of inverse modeling of travel times for determination of the P-wave velocity (Vp). The migration section of the single channel seismic data is used to define the model horizons and help to control their geometry. Wide angle hydrophone data of OBS are used to determine P-wave travel times. The picked travel times from various shots are inverted for P-wave interval velocities using RayInvr, which calculated theoretical travel times via ray tracing. Damped least squares optimization is performed to fine tune the fits between observed and calculated travel times. In the end, the Vp curve is achieved and the results are compared with that derived from the conventional hyperbolic curve velocity analysis method, the shape of the two curves are similar, and the velocity increases in the layer where gas hydrates are present.     

基于土地集约利用水平的城市热岛效应影响因子分析

利用乌鲁木齐市1990年8月6日、2001年7月11日和2013年7月4日三期热红外遥感数据,定量反演亮度温度,利用均值-标准差法对温度进行分级后评价城市热岛效应时间变化特征:1990、2001、2013年3个时段,乌鲁木齐市最大热岛强度分别为11.48℃,12.06℃和14.01℃;加权平均热岛强度分别为1.033℃,1.603℃,1.236℃;城市热岛比例指数分别为0.1054,0.3945和0.4328;乌鲁木齐市城市热岛效应在平均水平上呈现先增强、后减弱的趋势,最大热岛强度、热岛比例指数均增加,城市已初步形成区域内的"热岛",城市高温区温度将继续上升且面积存在扩展趋势.利用PSR模型构建指标体系对城市土地集约利用水平进行评价,在同时段内乌鲁木齐市城市土地集约利用水平和协调性均在提高.从评价结果出发,通过相关分析,得出基于土地集约利用的城市热岛效应影响因子包括:人口密度、建成区绿地覆盖率、地均国民生产总值、人均建设用地面积、人均道路铺装面积、地均固定资产投资,这些因子涵盖了经济、社会、环境三个方面.