Phylogenetic diversity of planktonic bacteria in the Chukchi Borderland region in summer

Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro-teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.     

Study on Ship Automatic Berthing System with Mooring Lines

This study describes an automatic berthing system with mooring lines. It is designed to be berthed by using mooring device on the upper deck of a ship. It is to berth once maintaining parallel with the quay by controlling both forward and aft breast lines. Berthing method is used through length adjustment of mooring lines connected between ship and quay by controlling the angular velocity and the torque of hydraulic motor in mooring device. The study is conducted under three changing conditions of draft, such as even-keel, rise of the gravity center and trim to stern. Variables affecting berthing stability are determined based on the control performance of each condition. Bond graphs method is used to model the system. Controller is designed as PID control method of reference-model algorithm. The control program is composed of synchronous control system based on the equations derived with the numerical analysis. The tank test is conducted to verify the usefulness of the control program.     

饥饿对方斑东风螺形态和组织生化成分的影响

以室内实验测定了方斑东风螺（Babylonia areolata）在饥饿120d过程中形态、组织生化成分及RNA/DNA比值的连续变化.结果显示,饥饿前期幼螺体重变幅较小,肝体比与体壳比下降迅速,三者分别于饥饿80、40、20d时显著地低于对照组,至饥饿120d时其降幅分别为22.33%、44.02%、30.39%.方斑东风螺在饥饿过程中,螺足肌与肝胰脏的水分含量分别从80.19%、48.44%上升至89.68%、63.97%;灰分含量分别从11.35%、8.92%增加至22.80%、16.52%;肝胰脏蛋白质含量从53.18%上升至56.65%.饥饿40d时,其足肌、肝胰脏脂肪含量均显著低于对照组,糖原含量在其饥饿20、40d时也显著地下降.饥饿120d时,其足肌的脂肪和糖原含量相应降幅分别为32.22%、44.64%,而肝胰脏的脂肪和糖原含量相应降幅则分别为26.87%、47.17%.足肌蛋白质含量在其饥饿80d前较稳定,后期迅速下降,实验结束时降幅为8.61%.这2种组织的RNA/DNA比值也呈逐渐下降趋势.上述结果表明,在饥饿状态下幼螺主要消耗这2种组织中脂肪与糖原的供能,当禁食长达80d后则加大对足肌蛋白质的动用,而相对保留肝胰脏蛋白质.组织含水量、RNA/DNA比值均可作为方斑东风螺饥饿状态下的营养预测指标.     

海滩剖面模拟试验

引言在过去的几十年间,已经从试验研究和现场测量中收集了有关海滩剖面的性质变化及引起海滩剖面变化的各种物理因素的大量资料信息。根据许多波浪槽试验以及现场观测来看,一般公认的有两种代表性的海滩剖面--阶梯式和沙坝式。Johnson（1952）给阶梯式取名为夏季海滩,沙坝式     

日本中部近海千叶沉积盆地铫子山嘴坳陷内的似海底反射层（BSR）之成因及意

在日本中部近海千叶（Ｃｈｉｂａ）沉积盆了铫子山嘴（ＣｈｏＳｈｉＳｐｕｒ）顶端的一个小型坳陷内,中新世－上新世内的Ｍｉｒｕｒａ组发育了似海底反射层（ＢＳＲ）。我们假设天然气水合物ＢＳＲｓ总是与位于气本水合物稳定基底（ＢＧＨＳ）处气体水合物层以及下伏的游离气同时形成,想必具ＢＳＲ标志的天然气水合物之形成与热成因的天然气有关,天然气与ＢＧＨＳ的上移相伴生,可假定这些后生的天然气是由地些未发现的,下伏的,     

Study of A Geo-Acoustic Model of Gas-Bearing Sediment and Its Application in Sediment with Low Acoustic Velocity

A new geo-acoustic model for gas-bearing sediment is proposed based on the work of Dvorkin and Prasad, and Biot theory. Only five geophysical parameters: sediment mineral composition, free gas saturation, tortuosity (also known as the structure factor), permeability, and porosity, are considered in the model. A benefit of this model is that we need only five parameters instead of ten parameters in the Biot’s formulas for acoustic velocity and attenuation calculation. Here the model is demonstrated with the in-situ experimental data collected from the Hangzhou Bay, China. The results of this study suggest that free gas content in sediment is the most critical condition resulting in a low acoustic velocity (compressional wave). The respective contributions of the other four parameters in the model are also discussed.     

Diagnosis of trace metal contamination in sediments: the example of Ensenada and El Sauzal, two harbors in Baja California, Mexico

Total metal concentrations in sediments from within Ensenada and El Sauzal Harbors are generally higher than at the mouths. Grain-size analyses suggested that this enrichment could be due to the presence of fine-grained sediments in the inner part of the harbors rather than to anthropogenic perturbations. The (Me/Al)(sample) ratios for Pb, Co, Ni and Fe were significantly higher for Ensenada Harbor relative to El Sauzal Harbor, whereas the ratios for Cd, Mn, Zn and Cu were statistically equivalent for both harbors. Calculated enrichment factors [EF(Me)=(Me/Al)(sample)/(Me/Al)(shale)] indicated that the metals showing slight enrichment were those associated with anthropogenic contamination (Pb, Zn), or probably related to primary productivity in the water column (Cd, Co). The levels of most of the metals were not greatly enriched, a consideration that is of the utmost importance when contamination issues are at stake.     

Modelling of sediment movement in the surf and swash zones

Under the action of marine currents, non-cohesive sediments evolve by bed-load, by saltation or suspension depending on their granulometry. Several authors have considered that the movement of sediment...     

水稻发育对光周期和CO_2浓度的响应

地球大气中CO_2浓度的增加已引起了人们对CO_2对植物的影响,尤其是对世界粮食供应的未来展望的兴趣。水稻是一种主要的粮食作物,人们对CO_2浓度在水稻生理生长期和整个生育期方面的影响了解相对较少,而这是一个水稻品种对一特定地区环境适应性的一个重要方面。本研究的目的是确定一个当代改良水稻品种(Oryza sativa,品种 IR-30)在两个对照的光周期下对不同CO_2浓度的响应。在整个生育期中,水稻植株生长在置于室外、采用自然光照并由计算机控制环境条件的人工气候室中。室内的CO_2浓度分别为160、250(低于环境浓度)、330(环境浓度)、500、660和900(高于环境浓度)μmol CO_2/mol 空气。在1987年,整个试验进行了两次。第一次即早稻试验在营养生长阶段内延长了光周期,而第二次即晚稻(LPR)试验只利用自然出现的光周期。在两个试验中,营养生长期的主茎叶片发育速率均比生殖生长期的主茎叶片发育速率快,同时在营养生长阶段,出叶速度随CO_2处理而加快。在晚稻试验中,与等于环境CO_2浓度和低于环境CO_2浓度的处理相比,高于环境CO_2浓度处理下的水稻幼穗分化和孕穗期出现较早,且整个生育期缩短。这种随CO_2浓度上升而产生的植物发育加速现象与CO_2引起的营养生长阶段内主茎叶片的减少有关。相对于晚稻试验,在早稻试验中水稻植株发育对CO_2的响应的减弱是由人为延长光周期迫使生殖生长发育阶段推迟引起的,在高于环境浓度处理中更是如此。鉴于全球大气中CO_2浓度的持续增大,CO_2导致的发育加速和全生育期缩短可能会成为一个参与挑选水稻品种和安排特定地区农事活动的水稻学家和育种学家感兴趣的问题。     

2011年3月胶州湾表层海水pCO2及海-气界面通量

依据2011年3月4日对胶州湾走航连续实测所得pCO2数据,结合水文、化学和生物等要素的同步实测资料,对胶州湾海域pCO2分布及其影响因素进行了初步探讨,并估算了3月海-气CO2通量。结果表明:3月胶州湾表层海水pCO2实测值在191～332μatm之间,平均值为278μatm,海-气CO2通量在-22.76～-7.13mmol·m-2·d-1,平均值为-14.2mmol·m-2·d-1,这一时期胶州湾从大气吸收约1.59×103t C,表现为大气CO2的强汇。生物活动是影响这一时期表层海水pCO2分布的主要原因。