Contamination of organotin compounds and imposex in molluscs from Vancouver,Canada

Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

地学组织面临的新挑战——CCOP专题会议报告

“地学组织面临的新挑战以及对其现状和组织结构的影响”专题会议于 2 0 0 1年 1 0月 2 6日在柬埔寨首都金边与 CCOP第 3 8届年会同时召开。来自 CCOP成员国和合作国 /组织的代表出席了会议。会议由 CCOP名誉顾问 YoshihikoShimazaki博士和何起祥教授主持 ,中国的蒋仕金先生被选为本报告的撰写人。参加会议的有柬埔寨、中国、印度尼西亚、日本、马来西亚、泰国、越南和菲律宾的代表 ,也有来自德国、瑞典、得克萨斯农工大学和荷兰的代表。虽然各国和地学研究状况不同 ,但是整个地区的地学组织面临的挑战是共同的。 CCOP成员国为了满…     

Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

北海盆地第三纪深海扇的定量分析

北海盆地第三纪深海扇的定量分析Ｔ．Ｒｅｙｎｏｌｄｓ深海扇内富存有大量的碳氢化合物。因此，无论是从扇容积作为石油储层还是在生产期提供水的补给方面来说，它们的规模、几何形态、砂的体积、纯砂体积与总体积之比以及砂的连通性都是石油工业的关键问题。但是，至今深...     

沿海与内陆地下卤水对比分析

分析对比我国滨海与内陆地下卤水的分布、成因及其水文地球化学特征，指出不同成因的地下卤水具有不同的水质．     

闽南—台湾浅滩渔场的上升流演变及其与渔业的关系研究

本文根据1975月7月温盐资料,证实了在渔场存在多单元上升流,兄弟屿和南澎列岛附近分别是沿岸和外海上升流最强海区。我们认为在外斜—外深一带出现高营养盐的愿因是,在上升流期,由于垂直外海环流环演变,而导致南海底层冷水上升与爬升(或涌升)多次交替现象所产生的结果。在埃尔尼诺年灯围产量锐减,在渔场夏季只出现一单元上升流,且上升流弱(除1965—1966年8—9月外),反之,在非埃尔尼诺年(除印度发生干旱和非埃尔尼诺头一年外)灯围产量高或较高,夏季都出现多单元上升流,且上升流强。此外还阐明了灯围产量与多单元上升流时间具有相关性,而与其次数具有逆相关性。     

南极沿海伯尔顿盐湖双刺镰状水蚤盐度耐性的季节变化

双刺镰状水蚤Drepanopus bispinosus为南极沿海伯尔顿盐湖(Burton lake)中的越冬种类之一,其雌性性成熟大约于每年的初冬季节,在随后8个多月的生活史中,经历了一个盐度从33(5月份)到39(11月份)的环境变化.本文报道了该水蚤雌性成体对环境盐度适应的季节差异,动物在冬季的耐低盐能力较夏季强,从冬季到夏季,动物对盐度的适应范围明显地向高盐度方向偏移,这与环境盐度对动物的驯化有关.在低盐水中,动物的呼吸率增高,且夏季大于冬季,因而进一步证实了环境盐度的变化对动物盐度适应性的影响.     

胡安·德富卡洋中脊热液体系中地震引起的变化

众所周知 ,太平洋东北部洋中脊的热液排放受地震扰动的影响 ,但是这些扰动起因于海底火山活动 ,直到现在 ,也没有观察到热液体系受非岩浆作用的构造事件的影响。这里我们报道了１９９９年６月在明显为构造成因的震群之前、期间和之后从胡安·德富卡海岭的几个热液喷口测量的热液排放的温度。排放流体温度在第一次地震之后４～１１ｄ开始上升 ,第一次上升之后 ,在到达较高温度值之前喷口温度波动了约一个月。我们也观察到了从热液体系中输出的流体在至少８０ｄ内提高了１０倍 ,并沿着整个海岭段延伸。与相对温和的构造活动对应的这样一个大的…