脊尾白虾(Exopalaemon carinicauda)维甲酸X受体基因克隆及其在温盐胁迫和蜕皮周期中的表达分析

为了明确脊尾白虾维甲酸X受体基因在环境胁迫和蜕皮周期中的作用,本研究通过RACE技术克隆了脊尾白虾(Exopalaemon carinicauda)维甲酸X受体c DNA全长,命名为Ec RXR基因,该c DNA序列全长为1323 bp,开放阅读框(ORF)为855 bp,编码一个284个氨基酸组成的蛋白质,分子量为30.918 k Da,理论等电点为PI为6.788;Ec RXR基因推导氨基酸序列经Blastp在线比对分析显示,Ec RXR与已知甲壳动物RXR的同源性为71%—90%;系统进化树分析结果显示,脊尾白虾Ec RXR的氨基酸序列与日本沼虾RXR的氨基酸序列聚为一支。荧光定量RT-PCR分析表明,Ec RXR基因在各组织中均有表达,而在眼柄相对表达量较高,血淋巴中最低。Ec RXR基因在蜕皮周期中的表达规律表明,Ec RXR基因表达在不同蜕皮时期存在明显变化,在眼柄、肝胰腺、胃和肠中整体呈现上升趋势,在鳃中呈现先下降后上升的趋势,在表皮中呈现逐渐降低的趋势。Ec RXR基因在温度、盐度胁迫过程中的表达规律分析结果发现,温度、盐度胁迫均可显著改变Ec RXR基因在鳃和肝胰腺中的表达模式,温度胁迫下Ec RXR基因在鳃中呈先上升后下降的表达趋势,肝胰腺中整体呈先上升后下降再上升再下降的表达趋势;盐度对鳃和肝胰腺的调控模式相同,均呈先升高后下降的变化趋势。本研究结果表明Ec RXR基因在脊尾白虾蜕皮发育、酶活性调控及渗透压调节中发挥重要作用,为深入研究甲壳动物维甲酸X受体基因的功能提供了重要的基础信息。     

Numerical modeling of ocean hydrodynamics with variational assimilation of observational data

Models and methods of the numerical modeling of ocean hydrodynamics dating back to the pioneering works of A.S. Sarkisyan are considered, with emphasis on the formulation of problems and algorithms of mathematical modeling and the four-dimensional variational assimilation of observational data. An algorithm is proposed for studying the sensitivity of the optimal solution to observational data errors in a seasurface temperature assimilation problem in order to retrieve heat fluxes on the surface. An example of a solution of the optimal problem of the World Ocean hydrodynamics with the assimilation of climatic temperature and salinity observations is offered.     

基于同步风场的机载SAR海浪参数反演方法

针对机载合成孔径雷达(SAR)对海探测特点,采用多入射角法从SAR数据本身得到与海浪参数反演区域时空匹配的同步海面风速和风向,并结合线性变换关系,计算得到海浪初猜谱对应的仿真SAR图像谱,将仿真SAR图像谱和观测SAR图像谱输入代价函数中进行迭代运算,通过非线性方程的解算得到最适海浪谱;采用交叉谱法去除海浪传播180°方向模糊,最终得到海浪参数。论文提出的基于同步风场的机载SAR海浪参数反演方法,充分利用了机载SAR海洋环境探测的优势,解决了传统SAR海浪参数反演中初猜谱构造依赖外部风场的问题,机载同步飞行试验的海浪参数反演结果与浮标观测值的有效波高、波向的均方根误差分别为0.23 m和13.23°,验证了该方法的有效性,可为机载SAR海浪参数反演业务化提供支持。     

Molybdenum Mass Fractions and Isotopic Compositions of International Geological Reference Materials

The double‐spike method with multi‐collector inductively coupled plasma‐mass spectrometry was used to measure the Mo mass fractions and isotopic compositions of a set of geological reference materials including the mineral molybdenite, seawater, coral, as well as igneous and sedimentary rocks. The long‐term reproducibility of the Mo isotopic measurements, based on two‐year analyses of NIST SRM 3134 reference solutions and seawater samples, was ≤ 0.07‰ (two standard deviations, 2s, n = 167) for δ^98/95Mo. Accuracy was evaluated by analyses of Atlantic seawater, which yielded a mean δ^98/95Mo of 2.03 ± 0.06‰ (2s, n = 30, relative to NIST SRM 3134 = 0‰) and mass fraction of 0.0104 ± 0.0006 μg g^?1 (2s, n = 30), which is indistinguishable from seawater samples taken world‐wide and measured in other laboratories. The comprehensive data set presented in this study serves as a reference for quality assurance and interlaboratory comparison of high‐precision Mo mass fractions and isotopic compositions.     

中国省际人口迁移的多边效应机制分析

区际人口迁移不仅与迁出地和目的地的要素特征以及距离有关,而且还受到周边迁移流的影响.基于网络自相关理论,利用"六普"省际人口迁移数据和相关统计资料,在重力模型的基础上考虑迁移流之间可能存在的几种空间依赖形式,构建中国省际迁移流的空间OD模型,初步揭示区域经济社会等因素及其空间溢出效应对省际人口迁移的影响,并就区域要素变化对整个省际人口迁移系统产生的"连锁反应"进行了模拟.结果表明:① 中国省际迁移流之间存在显著的网络自相关效应.目的地和迁出地的自相关效应皆为正,导致迁入和迁出流的空间效仿行为;迁出地和目的地周边则出现负的自相关效应,导致迁移流的空间竞争行为;② 区域经济社会等因素通过网络空间关系对周边地区产生的多边溢出效应导致迁移流在空间上集聚.其中,距离衰减效应位居各要素之首,其溢出效应进一步加剧距离的摩擦作用;对目的地而言,区域工资水平和迁移存量超过GDP的影响并产生正的溢出效应,促进周边地区吸引更多的外来人口;对迁出地而言,人口规模和迁移存量产生正的溢出效应,推动周边地区人口外迁;③ 区域要素变化潜在地对整个省际人口迁移系统产生一系列"连锁反应",震荡中心及其周边区域的迁移流波动较大.江苏省GDP增长5%的模拟结果表明,江苏迁往全国其他省份的人口数量都有不同程度地减少,而其他省份入迁人口均有所增加.相对而言,江苏周边省份的迁入或迁出流受到的波动较大,偏远省份波及较小,这是传统的重力模型所无法解释的.     

Unraveling the Peruvian Phase of the Central Andes: stratigraphy,sedimentology and geochronology of the Salar de Atacama Basin (22°30–23°S), northern Chile

The Salar de Atacama Basin holds important information regarding the tectonic activity, sedimentary environments and their variations in northern Chile during Cretaceous times. About 4000 m of high‐resolution stratigraphic columns of the Tonel, Purilactis and Barros Arana Formations reveal braided fluvial and alluvial facies, typical of arid to semi‐arid environments, interrupted by scarce intervals with evaporitic, aeolian and lacustrine sedimentation, displaying an overall coarsening‐upward trend. Clast‐count and point‐count data evidence the progressive erosion from Mesozoic volcanic rocks to Palaeozoic basement granitoids and deposits located around the Cordillera de Domeyko area, which is indicative of an unroofing process. The palaeocurrent data show that the source area was located to the west. The U/Pb detrital zircon geochronological data give maximum depositional ages of 149 Ma for the base of the Tonel Formation (Agua Salada Member), and 107 Ma for its middle member (La Escalera Member); 79 Ma for the lower Purilactis Formation (Limón Verde Member), and 73 Ma for the Barros Arana Formation. The sources of these zircons were located mainly to the west, and comprised from the Coastal Cordillera to the Precordillera. The ages and pulses record the tectonic activity during the Peruvian Phase, which can be split into two large events; an early phase, around 107 Ma, showing uplift of the Coastal Cordillera area, and a late phase around 79 Ma indicating an eastward jump of the deformation front to the Cordillera de Domeyko area. The lack of internal deformation and the thicknesses measured suggest that deposition of the units occurred in the foredeep zone of an eastward‐verging basin. This sedimentation would have ended with the K‐T phase, recognized in most of northern Chile.     

Activated sludge acclimation for toluene and DEHP degradation in a two-phase partitioning bioreactor

The effect of activated sludge acclimation on the biodegradation of toluene in the presence of a biodegradable non-aqueous phase liquid, di (2-ethylhexyl) phthalate (DEHP), in a two-phase partitioning bioreactor was characterized. The influence of the presence of DEHP, at a ratio of 0.1 % (volume ratio), and of the acclimation of activated sludge (AS) on the biodegradation of hydrophobic VOC was studied. AS was acclimated to both toluene and DEHP simultaneously. Using acclimated cells, 73 and 96 % improvement of the mean biodegradation rates was recorded for toluene and the organic solvent (DEHP), respectively, if compared to the values recorded in the absence of acclimation, during tests performed in Erlenmeyer flasks. Degradation rates were further improved by the use of acclimated AS in a reactor with a large head space; degradation yields for toluene and DEHP were above 99 and 89 %, respectively.     

Assessment on characteristics of LUCC process based on complex network in Modern Yellow River Delta,Shandong Province of China

Research on land use cover change (LUCC) has reached a mature stage and has formed a relatively complete scientific system. However, most studies analyzed the LUCC process from the view of land use types ignoring the integrality and systematicness of the land use system, which brought certain constraints to understanding the complexity and systematicness of land use process scientifically. Using the Modern Yellow River Delta (MYRD) of China as the study area, this research introduced the complex network analysis method to the study of the LUCC process by using 9 periods of land use data between 1976 and 2014 to build 8 stages of complex networks. The values of node degree, betweenness, changing proportions, and average shortest path in the networks were calculated to identify the key land use types, changing models of land use types, and the stability of land use system. Additionally, main parts of complex networks of the period from 1976 to 1995 and the period from 1995 to 2014 were selected to evaluate the changing characteristics of the LUCC process. The results indicated the area and proportion of natural wetland kept reducing, but the area and proportion of artificial wetland or non-wetland continued rising. The bare land, reed, bush, and cultivated land were the key land use types of the LUCC process. In the past 38 years, beach, bare land, reed and bush had been the output types, and the building land and salt pan had been the input types. The LUCC process has been a transfer process of natural wetland to artificial wetland and non-wetland in the past 38 years, which could be divided into land accretion process (1976 to 1995) and construction process (1995 to 2014). The land ecosystem was unstable for the period from 1990 to 2006.     

海洋磁力仪性能指标分析与测试

海洋磁力仪性能测试和评价是海洋磁力测量前期技术准备的重要内容,也是当前海洋磁力测量规范要求的薄弱环节。研究了海洋磁力仪的性能指标的具体含义,提出了切合实际的海洋磁力仪性能指标测试内容、流程、方法和要求,采用G-882G SX海洋磁力梯度仪实测数据,计算了海洋磁力仪动态噪声、内符合精度和外符合精度等关键指标,为海洋磁力仪性能测试工作提供了技术指导,也为海洋磁力仪国产化中面临的性能指标论证和设计提供了依据。     

浮托安装进船过程中护舷碰撞力实测研究

浮托安装法广泛应用于大型组块海上安装。导管架平台上部组块浮托安装进船过程中,风、浪、流引起的浮托驳船横向运动造成浮托驳船与导管架桩腿的碰撞,碰撞力可能会对导管架结构造成损伤。陆丰7-2上部组块浮托安装中,为了监测碰撞力大小,设计了碰撞力海上监测系统。通过在导管架外侧四个桩腿上安装光纤光栅应变传感器对碰撞过程中导管架桩腿进行应力监测,进而计算碰撞力。对碰撞过程,载荷作用位置、方向进行简化,并对载荷大小及垂向作用位置对计算的影响进行了研究。结构分析模型简化后,测点von-Mises应力与碰撞力大小成正比,对导管架整体结构建模计算并取局部结构计算比例系数,结合应力实测数据计算出进船过程中驳船对导管架桩腿碰撞力。