Total oxyradical scavenging capacity of the deep-sea amphipod Eurythenes gryllus

Environmental concern for the deep-sea ecosystem is increasing as contaminants, originating from anthropogenic activities, have been detected in deep-sea biota. However, little is known on the xenobiotics metabolising capability of deep-sea fauna. In this study, the deep-sea amphipod Eurythenes gryllus was selected as sentinel species to measure the total oxyradical scavenging capacity (TOSC). Individuals of E. gryllus were sampled at 2000 m depth in the Arctic Ocean. The TOSC assay was measured on the cytosolic fraction and the soluble fraction (3 kDa) of the digestive gland and on the cell-free haemolymph toward peroxyl, hydroxyl and peroxynitrite radicals according to the method of Winston et al. [Free Radical Biology and Medicine 24 (3) (1998) 480] and Regoli and Winston [Toxicology and Applied Pharmacology 156 (1999) 96]. These results provide the first baseline data set for total antioxidant capacity in a deep-sea amphipod.     

Numerical experiments on double-diffusive intrusions in the ocean and their relation to laboratory experiments

Numerical experiments on double-diffusive intrusions are reviewed briefly. Though the number of studies is very limited at present, they have undoubtedly an advantage that a heat–salt system can be studied without undesired heat loss from the boundaries.Several possibilities for future numerical experiments are summarized.     

An experimental pressure compensated wet suit

Personal thermal insulation by means of neoprene from wet suits provides adequate temporary control of body heat loss only at the shallow end of the air diving depth range, but it constitutes by far the most popular approach to diver thermal support. Howewer, compression of the trapped gas phase in neoprene foam seriously reduces its insulation performance on exposure to high ambient pressure. With conventional wet suits equipped with heating, the necessary level of power required at depths greater than about 30 m is too high, and also unsafe without the back up that increased insulation would provide. One approach to the goal of depth-insensitive insulation is to use a wet suit with a continuous internal gas space pressurized nominally to ambient pressure, so that its thickness remains substantially constant at all depths. The composite material properties required are: outer skins that are tough, flexible and free of pin holes; an open foam internal structure capable of resisting, without significant dimensional change, the relatively small pressure changes that occur over the height of a man; and a high bond strength.Samples of a composite material that meets these requirements have been developed, and a prototype suit has been successfully fabricated by conventional techniques.