Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi

The role of endosymbiotic diatoms as pro-oxidant stressors in porifera has been investigated in the Antarctic sponge Haliclona dancoi in which the presence of diatoms is influenced by marked seasonal variations during the austral summer. Both chlorophaeopigments and frustules were absent in sponge tissues sampled in early November at the beginning of the summer and increased from the mid of December with slightly shifted temporal trends. The efficiency of antioxidant defenses in the sponge showed a marked response to symbionts with clearly enhanced values corresponding to the peak of diatoms.     

Inverse reconstructions of ecosystem flows in investigating regime shifts: impact of the choice of objective function

Inverse analysis is increasingly used in ecosystem modelling to objectively reconstruct a large number of unknown flows or interactions from a small number of observations. This type of analysis may be useful in relating observed regime shifts in ecosystem structure to underlying processes. Inversions of ecosystem flow networks currently use a constrained least-squares solution which at the same time minimizes the squared norm (the sum of squares) of the reconstructed flows. This minimum norm (MN) inversion is thought to be a parsimonious solution to the ecosystem flow inverse problem, but it may well not reflect how ecosystems are organised. It has been proposed instead that ecosystems evolve to maximize energy/mass flows or that they maximize the information content of the network weighted by ecosystem flows (ascendancy). We used simulated inverse experiments, where inverse analyses are applied to simulations of flow networks, to explore objective functions different than the MN generally used. We could not compute inverse solutions that maximize ascendancy because the objective function is unbounded. We could calculate inversions that maximize flows; however, these generally overestimated the simulated flows, even though the simulations were designed to maximize flows. It appears that the ecosystem flow inverse problem is too under-determined (too few data relative to the number of unknowns) to allow the use of these maximizing goal functions. We introduce a new minimization that simultaneously minimizes the squared flows and the squared differences between flows. This smoothing minimization makes the inverse flows as even as possible and it helps with some technical issues with MN inversions. The simulated inverse experiments indicated that this smoothed norm (SM) is the most robust in comparative analyses of contrasting ecosystem states, such as those that can be associated with regime shifts. Like the MN inversion, the SM inversion has no ecological basis. However, it is a conservative norm that is less likely to produce false differences between the dynamics of regimes.     

Alkaline phosphatase activity and its relationship to inorganic phosphorus in the transition zone of the North-western African upwelling system

The enzymatic activity of alkaline phosphatase (APA) was studied in the transition zone between the African upwelling system and the open ocean waters of the Canary Islands region. This region is recurrently dominated by the presence of upwelling filaments that may transport nutrient-enriched waters out into the open ocean before nutrients become exhausted by plankton. Turnover rates by APA were generally low in the whole region, but detectable in all the measurements carried out. On average, turnover rates were higher in the upwelling stations, and APA in those waters seemed to be mainly generated by heterotrophic bacteria to supply easily assimilable organic C. APA outside the upwelling area showed an inverse hyperbolic relationship with increasing phosphate, suggesting the presence of both constitutive and Pi-inducible APA. In these offshore waters, a threshold of 0.1 μM of phosphate could be defined for the regulatory function of Pi on APA. Thus, APA in nutrient-poor waters seemed to be induced to compensate for Pi-deficiency. Turnover rates in the filaments showed basal (probably constitutive) levels, whereas they increased in the surrounding waters, where phosphate concentration presumably did not satisfy plankton P-demands. The fertilising effect of the filaments and associated cyclonic eddies extended to at least 175 km offshore, where basal alkaline phosphatase activities were still found. The magnitude of this effect depends probably on the intensity of upwelling events and the degree of recirculation of filament water back to the coastal jet.     

Mechanisms of mud extrusion on the Mediterranean Ridge Accretionary Complex

 Drilling two mud domes on the Mediterranean Ridge during ODP Leg 160 has demonstrated that the eruption of mud breccia began at least 1.5 Ma ago. An evolution through extrusive building of a cone, followed by successive eruptions of clast-bearing mud debris flows and subsequent subsidence can be deduced for both domes. Results from permeability and shear strength tests, grain size analyses, sedimentary textures, and clast provenance provide clues concerning the mechanism of mud volcanism. The collision of Africa with Eurasia resulted in backthrusting of the evaporite-dominated accretionary wedge against a rigid backstop. This allowed egress of overpressured fluid-rich mud of presumed Messinian age from the décollement, although many of the clasts may have originated from the overlying accretionary wedge.     

Numerical modelling of the propagation of tsunami waves in the crimean peninsula shelf zone

The paper analyses the effect of non-linearity and bottom friction on propagation of tsunami-type surface waves from the abyssal part of the Black Sea towards the shelf zone. The study relies, on numerical solution of unidimensional non-linear equations for long waves, using the finite-difference technique. Numerical experiments have been conducted for the bottom profile continental slope and shelf, with the full wave reflection being prescribed at a 10-m depth contour. It has been shown that the major role in transforming solitary waves belongs to non-linear topographic factors rather than to dissipation. The reflected wave has been found to be non-linearly distorted, and wave heights in the Black Sea coastal zone have been found to increase by many times. Translated by Vladimir A. Puchkin.     

Tectonic setting of the Azores Plateau deduced from a OBS survey

The studies of Azores seismicity generally show shocks with either normal faulting or right-lateral strike-slip along the ESE direction, compatible with a eastward relative motion of the Eurasian (EU) relative to the African (AF) plate. However, the 1 January 1980 earthquake was interpreted as a clear left-lateral strike-slip shock along the N150E direction. This pattern is difficult to explain in terms of the relative motion between the EU, AF and North American (NA) plates: all available models for the present day movement of this triple junction fail to explain the regional variability in the stress conditions of the area. Here we present data from a 34-day long Ocean Bottom Seismograph array deployment. We show that the seismicity is distributed along a band aligned with the island chain itself, and is concentrated along several faults with an approximate N150E strike, cutting the Azores plateau in all the area covered by the OBS network. The combination of these new results with other geophysical data permits us to conclude that the tectonic setting of the Azores plateau is characterised by the existence of two sets of faults, in the N120E and N150E directions, defining several crustal blocks, whose relative motion accommodates the interaction of the three megaplates. The deformation of these tectonic blocks is probably driven by the shear between the EU and AF plates. This model explains well the spatial variability of the stress conditions in the Azores domain, the combination of dextral and sinistral strike slip mechanisms and the observed seismotectonics of the Azores islands.     

Full scale decay test of a tanker: field data and theoretical analysis

This paper presents results from a full scale decay test made with a tanker in a relatively protected area in the Brazilian coast. In at least two tests the environmental loads (wind, waves and current) were very small and the time history of the surge motion was well behaved, making it possible to check some proposed models for the damping in the hull and mooring lines. Field data seem to confirm that the damping is indeed of the fluid viscosity type and the theoretical models are able to recover roughly 75% of the observed damping, the energy dissipation in the mooring lines being, by far, the major contribution. The remaining 25% are likely due to non modeled effects, such as the environment influence, which although small and not measured certainly exists, and to the friction between the mooring lines and the seabed.